Which of the following is an advantage of using electrons in cryo-EM as opposed to X-ray electromagnetic radiation in crystallography. Check all that apply retain amplitude information permit to obtain charge density distribution O magnification of the signal due to lattice effects V retain phase information
Which of the following is an advantage of using electrons in cryo-EM as opposed to X-ray electromagnetic radiation in crystallography. Check all that apply retain amplitude information permit to obtain charge density distribution O magnification of the signal due to lattice effects V retain phase information
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Transcribed Image Text:Which of the following is an advantage of using electrons in cryo-EM as
opposed to X-ray electromagnetic radiation in crystallography. Check all that
apply
retain amplitude information
V permit to obtain charge density distribution
magnification of the signal due to lattice effects
V retain phase information
Which of the following are NOT important in post-image analysis. Check all
that apply
sufficiently large number of images for correct image reconstruction
O determination of relative sample orientation
back projection of your image
O reconstruction of phase information similar to X-ray crystallography process
Which of the following methods are NOT used for cryo-EM image
reconstruction
backprojection
fourier inversion
multiple rounds of refinement of your model
use of a known homologous structure as a refererence
splitting your data set in half ( or more) parts and working with each one individually
to have a cross-check validation of your model
O all of the choices are used in image reconstruction strategies
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