Answered: Which RE’s from table below have a 5’…

Name: After restriction enzymes cut, they contain unpaired bases. Type II re
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Description: After restriction enzymes cut, they contain unpaired bases. Type II restriction enzymes leave ends that may be 5' overhanging, 3' overhanging, or blunt. In all cases each end is left with a 3' OH and a 5' phosphate. All blunt ends, and any compleme

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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After restriction enzymes cut, they contain unpaired bases. Type II restriction enzymes leave ends that may be 5' overhanging, 3' overhanging, or blunt. In all cases each end is left with a 3' OH and a 5' phosphate. All blunt ends, and any complementary overhanging ends may be re-ligated with T4 DNA ligase, as long as at least one 5'- phosphate is present.

In the tables below G^AATTC means that the end after cutting with enzyme will be:

-----G 3'

-----CTTAA 5'

GTGCA^C means that the end will be:

-----GTGCA 3'

-----C 5'

Which RE’s from table below have a 5’ overhang? Which ones have a 3’ Overhang?

AccI	GT^CGAC	BamHI	G^GATCC
ClaI	AT^CGAT	NsiI	ATGCA^T
PstI	CTGCA^G	BglII	A^GATCT
TaqI	T^CGA

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