Why is necessary to completely grind the beef in an LDH purification experiment? How do you prepare a 100mL of 0.1 M phosphate buffer? From the anterior buffer, how do you make 100mL of 0.05 M? Calculate the amount of ammonium sulfate necessary to get a 20% solution?
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Why is necessary to completely grind the beef in an LDH purification experiment?
How do you prepare a 100mL of 0.1 M phosphate buffer?
From the anterior buffer, how do you make 100mL of 0.05 M?
Calculate the amount of ammonium sulfate necessary to get a 20% solution?
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- Why is necessary to remove fat and tendons from the heart sample in a LDH purification experiment?What would be the best buffering agent to choose if you wanted to buffer an enzyme reaction or tissue culture medium at pH = 7.95? Group of answer choices a. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); pK = 7.5 b. Histidine; pK1= 1.78; pK2= 5.97; pK3= 8.97 c. Bicarbonate; pK = 6.1 d. Phosphate; pK1 =2.0, pK2 = 6.8; pK3 = 12.5 e. Tris or Tris(hydroxymethyl)aminomethane; pK = 8.07Consider a crude extract with a mixture of the 5 proteins listed below. Protein A 4.5 pl 160 molecular weight, kDa Protein B 12.5 pl and 65 molecular weight Protein C 5.0 pl, 15 molecular weight Protein D 6.8 pl, 150 molecular weight Protein E 9.5 pl, 45 molecular weight You load this protein mixture onto an anion exchange column at pH 11. Next, you apply a "washing" step by passing through buffer at pH 11. Finally, for your elution step, you apply a pH gradient starting from pH 11 to pH 2.0 (A gradient buffer system allows you to gradually and continuously change the pH of your mobile phase starting from pH 11 up to pH 2). You load this same protein mixture onto a Size Exclusion column. Please indicate the order in which these proteins will elute for both. Group of answer choices Yes or No, please explain your answer. If your protein of interest is protein A, would using anion exchange column be completely successful at separating it from all the other proteins? Group of answer…
- Which stationary phase is better in separation of the components of moringa extract for column chromatography? Sodium Bicarbonate or Silica Gel? And Explain.The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?What is Xanthan ? What is the importance of using Xanthan in the emulsion experiment?
- Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose solution?How much nystatin stock (50 mg/mL) needs to be added to 15 mL of tryptic soy agar to give a final concentration of 50 μg/mL?
- You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis. How much stock should you use?An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?How will you prepare a 5% BSA solution? Show all your steps.