Why is primase needed for DNA replication?

Q: What is meant by the semiconservative nature of DNA replication?

A: DNA replication is the process of producing daughter strands of DNA from parent strands by the…

Q: True or false: After chromosomal replication, Type I topoisomerase is used to coil up the…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: In DNA replication, what is the primer composed of and whyare there leading and lagging strands?

A: DNA replication is a biological process that results in formation of exact copies of DNA which is…

Q: Why do eukaryotes need multiple origins of replication?

A: Unlike prokaryotes, eukaryotic chromosomes also very often showed multiple origins of replication.…

Q: Explain in not more than 5 sentences. If deoxyribonucleotides that lack the 3’-OH groups are added…

A: Deoxyribonucleotides are the nucleotides which lacks a hydroxyl group at 2' OH of ribose sugar.…

Q: Prokaryotic DNA is normally slightly negatively supercoiled, which can lead to "DNA breathing". How…

A: DNA breathing is defined as the short and temporary or in other words a transient opening of nucleic…

Q: What is a replication fork? Why is it important in replication?

A: Replication is the process where the double-helical structure of DNA acts as a template for the…

Q: The topoisomerase enzyme catalyzes the interconversion of the relaxed and supercoiled forms of DNA…

A: DNA replication is the process of the formation of identical copies of DNA. It occurs in nucleus of…

Q: What protein is key in the termination process of DNA replication in E. Coli?

A: In molecular biology, DNA replication is the biological process of producing two identical DNA…

Q: Why did Okazaki propose that lagging strand synthesis proceeds through the synthesis of short…

A: The effective copying of double-stranded chromosomal DNA is required for cellular DNA replication.…

Q: How does the process of DNA replication generate mismatch mutations? What mechanisms are available…

A: DNA replication is the process in which dsDNA is copied to produce two identical DNA molecules.

Q: Which Strict Operating Requirements DNA Polymerase Has?

A: DNA polymerase is an enzyme involved in the synthesis of nucleotides. These are important for the…

Q: What is the action of Ribonuclease?

A: Ribonuclease:                        RNase ~ commonly abbreviated as Ribonuclease            A type…

Q: Why is primase required for replication?

A: The process by which a DNA molecule makes its identical copies is known as DNA replication. It takes…

Q: Which nucleotide is most non-polar and why? What structural element of DNA gets cleaved by…

A: The monomeric units of nucleic acids are called nucleotides. Nucleotides are generally…

Q: Escherichia coli (E. coli) has one replication origin that contains specific genomic sequences…

A: DNA replication It is defined as the process through which the copying of DNA takes place in cells.…

Q: What would happen if, in the course of replication, the topoisomerases were unable to reattach the…

A: Replication is the process of copying the DNA molecule to form two daughter DNA strands before the…

Q: What does DNA polymerase III need to catalyze DNA synthesis

A:

Q: Why is DNA gyrase necessary for replication?

A: DNA gyrase is an enzyme of class topoisomerase and subclass of topoisomerase type II. DNA…

Q: With regard to DNA replication, define the term bidirectional replication.

A: Deoxyribonucleic acid (DNA) is a double-stranded molecule, which consists of two strands of…

Q: Why are the activated nucleotides in nucleic acid polymerization reactions 5’-triphosphates?

A: According to the question, we have to explain the reason for the activated nucleotides in nucleic…

Q: What is the advantage of having the replication machinery in a complex?

A: Complex level of organization basically refers to the combination of organ systems in order to…

Q: What is the role of TOPOISOMERASES in DNA replication?

A: Topoisomerases (also called polymer topoisomerases) are enzymes that facilitate to wind or unwind…

Q: Explain why DNA replication requires RNA primers. What do they do and why do they have to be…

A: The process of duplicating a double-stranded DNA molecule into two identical DNA molecules is known…

Q: Why do we say that DNA replication is semiconservative?

A: DNA is the chemical name for the molecule that carries genetic instructions in all living things.

Q: Why would replication come to a halt in the absence of topoisomerase II?

A: Two identical copies of the original DNA are produced as a result of the DNA replication process. In…

Q: why is only one nucleotide added at the time during DNA replication?

A: DNA is the genetic material of almost all living organisms. It contains genetic information which is…

Q: Why must reoviral replication events occur within thenucleocapsid?

A: Viruses are minute particles that are made up of only their genetic material which may be DNA or RNA…

Q: How many subunits does the E. coli DNA polymerase I have? Soloct onot

A: Option(d) 1

Q: Insulin is synthesized as preproinsulin, which has 81 amino acids. How many heterocyclic bases must…

A: Insulin is synthesized from preproinsulin. The preprotein is then cleaved by a proteolytic enzyme to…

Q: What is the dogma for dna replication

A: In molecular biology ,the term central dogma explains the flow of genetic information from DNA to…

Q: Why are primers required in DNA replication but not in transcription?

A: A gene is a DNA-based functional heredity unit that delivers instructions for the production of RNA…

Q: Cytosine

A: Introduction :- A mutation occurs when a DNA gene is damaged or changed in such a way as to alter…

Q: Which statement is TRUE regarding the DNA ligase mechanism?

A: DNA ligases DNA ligases are the enzymes that are responsible for joining the two strands of DNA by…

Q: Why is it necessary for naturally occurring DNA molecules to be negatively supercoiled?

A: DNA supercoiling is important for DNA packaging within all cells.

Q: Why is a clamp loader necessary in replication?

A: Clamp loader was identified as DNA polymerase processivity factors. Clamps not only increase the…

Q: Which of the following enzymes has 5' to 3' exonuclease activity?

A: 5’ to 3 ‘ exonuclease activity is the ability to remove nucleotides by enzymes. The nucleotides are…

Q: What is replication slippage?

A: In molecular biology, DNA replication can be described as the process during which DNA is duplicated…

Q: What are replication licensing factors? How did they get their name?

A: Replication is a process by which the DNA is duplicated to form two identical copies of DNA using…

Q: What is the difference between primase and DNA polymerase alpha?

A: DNA is a polymer made up of two polynucleotide chains that coil around each other to form a double…

Q: What role do the following enzymes play in DNA replication? DNA polymerase         Helicase…

A: DNA replication is the DNA dependent DNA synthesis process. In this process a set of enzyme…

Q: In eukaryotes, the Replication factor C (RFC) is a clamp loader. In the absence of RFC, what would…

A: The Replication factor C (RFC) is the accessory proteins that's is essential for the processive DNA…

Q: In eukaryotes, what is meant by the term DNA replication licensing? How does the process occur?

A: BASIC INFORMATION DNA Replication  It is the process in which there is production of the replicas…

Q: Why are primers required in DNA replication but not in transcrip­tion

A: DNA replication is a process that takes place inside the nucleus of the cell. During replication the…

Q: Why is DNA replication is considered a semi-discontinuous process? Explain in detail.

A: Biomolecules are organic molecules present in living organisms. There are mainly four biomolecules.…

Q: Why does DNA synthesis start with an RNA primer

A: By extending an RNA primer in the 5' to 3' direction, DNA polymerase begins to synthesise a new DNA…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.