Why should the microscope be calibrated for each objective and prior to each use?

Q: What particular features of fluorescence make it useful for microscopy?

A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…

Q: Differentiate between the major types of optical microscopes, theirillumination sources, image…

A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…

Q: Which part of the microscope is used to adjust the light?

A: Microscope is an instrument which is used to see very tiny microscopic objects which cannot be seen…

Q: Why is CSLM able to view different layers in a thick preparationwhile bright-field microscopy…

A: The technical field that uses microscopes to observe objects and their areas that cannot be seen…

Q: Compare and contrast light microscopy and electron microscopy

A: Microscopy is the technical field of using microscopes to view objects and areas of objects that…

Q: What is the usage of the superresolution microscopy technique?

A: The microscope is identified as an optical instrument to observe very small objects. It is able to…

Q: What do you mean by deconvolution microscopy technique?

A: The organisms or substances that cannot be observed with unaided eyes are microscopic. The equipment…

Q: If you are to follow the movement of the organism through the microscope, how will you do this?

A: When a live sample is placed on the slide, it is possible to view the organisms in their living…

Q: What is the the total magnification of a microscope that is set up using a 10x ocular lens and a 20x…

A: A microscope is a scientific tool that is used to look at organisms or things that can't be seen…

Q: Why should you always begin to use a microscope with a low-power objective?

A: Microscope is an instrument used to see the objects that cannot be seen by naked eye. A light…

Q: What is the difference between a phase-contrast and differential interference-contrast microscope?…

A: Microscope is an instrument that gives large images of small objects, This allows an exceedingly a…

Q: List two ways that a stereoscopic dissecting microscope differs from a compound microscope

A: The stereoscopic dissecting microscope is often used to study the surface of specimens. The compound…

Q: For which types of specimens is darkfield microscopy preferred over brightfield microscopy?

A: Microscope is an instrument used to view objects and organisms that are too small to be seen by…

Q: Which of the following statements are true in the context of the microscope's field of view? I. It…

A: Microscope field of view (FOV) It is defined as the maximum area visible when looking through the…

Q: what would you do if you had to discard disposable microscope slide?

A: Disposable things are that can be thrown after its use. They are made up of material that can be…

Q: Compare bright-fi eld, dark-fi eld, phase-contrast, confocal, and fl uorescence microscopy as to fi…

A: Microscopy allows one to view samples and objects that cannot be seen with the human eye. With all…

Q: The resolution of a microscope could be increased by raising the condenser to its highest point.…

A: Resolution of a microscope is defined as the ability of a microscope to separate two closely spaced…

Q: What is the purpose of confocal microscopy technique ?

A: The microscope instrument is used to view the objects, which are not visible under the naked eye.…

Q: why is microtome important in microscopy?

A: Objects or organisms that cannot be viewed by the unaided eyes are studied under the microscope.…

Q: What are two things that can be done to improve contrast on a microscope?

A: Contrast refers to the darkness of the background relative to the specimen.

Q: What is the difference between a simple and a compound microscope?

A: A microscope refers to the laboratory instrument that is used to view tiny objects, which cannot be…

Q: Which microscope achieves the highest magnification and greatest resolution?

A: Out of all the microscopy technologies available, Electron Microscope is the best in terms of…

Q: What advantages do confocal and deconvolution microscopy provide in comparison to conventional…

A: Confocal microscopy uses light from laser, elimination of background information away from the focal…

Q: What are the dehydrating agents used for Electron Microscopy, and why?

A: Dehydration in microscopy is a technique of removal of water content from the sample specimen and…

Q: List  6 ways on the proper handling/manipulation of the microscope.

A: Microscope is an laboratorial instrument used for the magnification of micro objects which can not…

Q: Describe the process of bringing a specimen into focus using a compound light microscope.

A: A microscope is an instrument that can magnify specimen which is otherwise too small to be seen,…

Q: What part of the microscope allows you to move the stage up and down in small increments to aid in…

A: According to the question, we have to mention the part of the microscope that allows to move the…

Q: Describe the conditions under which dark-field microscopy is the best choice for viewing specimens.

A: Darkfield microscopy is a method of microscopy in which the images are excluded from the unscattered…

Q: Why is it important to center your object before moving to higher magnifications in a light…

A: A microscope is a crucial tool in the study of organisms that are very minute or to study the cells…

Q: What is the advantage of phase-contrast microscopyover bright-field microscopy?

A: Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light…

Q: Differentiate bright-phase and dark-phase microscopy

A: In the course of history of biology, various new tools and techniques have been developed to explore…

Q: What are the mechanical parts of microscope and their functions?

A: Many important anatomical features, particularly those that function at the tissue or cellular…

Q: Explain the differences between Brightfield, Darkfield, and Phase-Contrast Microscopy.

A: * Here are some differences between bright field microscopy darkfield miccroscopy and phase contrast…

Q: What ethical issues may arise from the use of microscopes?

A: A microscope enables us to view microbial lifeforms that are not visible to the naked eye. The…

Q: What common parts are found in both the compound microscope and stereo (dissecting) microscope?

A: Stereo and compound light microscopes both use visible light to create an image and are optical…

Q: Why is the resolution so superior in the electron microscope?

A: A beam of electrons that travel in wavelike patterns when accelerated to high speeds form the images…

Q: How is confocal scanning laser microscopy differentfrom fluorescence microscopy? In what ways are…

A: Microscope is an analytical instrument which is used to analyse, study and visualize internal…

Q: What are the different types of microscope? Describe each.

A: A device that enlarges the tiny particles or an object for a clear view. It can give a clear sight…

Q: What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical…

A: Microscope is the widely used laboratory instrument in the molecular biology, it is mainly used to…

Q: Why would specimens viewed with a compound microscope be thin and/or chemically cleared?

A: The human eye can see objects upto 0.1 millimeters. If the objects are smaller than this, the human…

Q: How does differential interference contrastmicroscopy differ from bright-field microscopy?

A: Microscopes are used to observe objects at cellular level such as the nucleus, mitochondria and…

Q: What are the different magnifications of an ordinary light microscope?

A: Microscopes are used to visualise and object in a magnified image. there are different types of…

Q: What are the two parts used to carry the microscope?  What does Total Magnification mean?

A: Microscope is used in laboratory settings to obtain a clear and precise view of the specimens under…

Q: How do stains used for light microscopy compare withthose used for electron microscopy?

A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…

Q: Always begin examing microscope slides with which power objective?

A: A microscope is an instrument of immense importance in a biology/ microbiology laboratory. As the…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.