You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas. Which operons would be functional under these conditions? What repressors would be made? (NOTE: name the repressors using their gene names) Which repressor(s) would be made in the inactive form? Which repressor(s) would be made in the active form? Which repressor(s) under these conditions can bind the operator sequence?
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The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
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You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas.
- Which operons would be functional under these conditions?
- What repressors would be made? (NOTE: name the repressors using their gene names)
- Which repressor(s) would be made in the inactive form?
- Which repressor(s) would be made in the active form?
- Which repressor(s) under these conditions can bind the operator sequence?
- Which repressor(s)under these conditions cannot bind the operator sequence?
- What effect does lactose have on the bacterial cell’s lac operon?
- What does the absence of glucose do to the bacterial cell?
- You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon?
- What kind of regulation does lactose provide to an operon’s repressor?
Briefly answer these questions Once you have answered these questions
3. Explain your answer for question #G above (What effect does lactose have on the bacterial cell’s lac operon?)
4. Explain your answer for question #H above (What does the absence of glucose do to the bacterial cell?)
Explain your answer for #I above (You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon?)
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