You are studying the role of cAMP in cell signaling. You hypothesize that 2 intracellular signaling proteins, named GO and TIGERS, interact with each other when cells are treated with an extracellular source of CAMP. The two proteins were tagged with CFP or YFP (CFP = cyan fluorescent protein; YFP = yellow fluorescent protein). CFP is excited by 435 nm light and emits lights at 480 nm. YFP is excited by 480 nm light and emits light at 535 nm. H 400 Fluorescence 500 Wavelength of Emitted Light 400 500 Wavelength of Emitted Light 600 600 Fluorescence 400 400 500 Wavelength of Emitted Light 600 500 Wavelength of Emitted Light 600 Fluorescence Intensity 400 500 Wavelength of Emitted Light 600 Figure Legend. A. Cells expressing GO-CFP irradiate with 435 nm light. B. Cells expressing TIGERS-YFP and irradiated with 435 nm light. C. Cells expressing TIGERS- YFP and irradiated with 480 nm light. D. Cells expressing GO-CFP and TIGERS-YFP and irradiated with 435 nm light. E.Cells expressing GO-CFP and TIGERS-YFP treated with CAMP and irradiated with 435 nm

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You are studying the role of CAMP in cell
signaling. You hypothesize that 2
intracellular signaling proteins, named GO
and TIGERS, interact with each other
when cells are treated with an extracellular
source of CAMP. The two proteins were
tagged with CFP or YFP (CFP = cyan
fluorescent protein; YFP = yellow
fluorescent protein). CFP is excited by 435
nm light and emits lights at 480 nm. YFP
is excited by 480 nm light and emits light
at 535 nm.
400
500
Wavelength of
Emitted Light
400
500
Wavelength of
Emitted Light
600
600
Fluorescence
400
400
500
Wavelength of
Emitted Light
600
500
Wavelength of
Emitted Light
600
Fluorescence
Intensity
400
500
Wavelength of
Emitted Light
600
Figure Legend. A. Cells expressing GO-CFP
irradiate with 435 nm light. B. Cells
expressing TIGERS-YFP and irradiated with
435 nm light. C. Cells expressing TIGERS-
YFP and irradiated with 480 nm light. D.
Cells expressing GO-CFP and TIGERS-YFP
and irradiated with 435 nm light. E. Cells
expressing GO-CFP and TIGERS-YFP treated
with CAMP and irradiated with 435 nm
light.
Transcribed Image Text:You are studying the role of CAMP in cell signaling. You hypothesize that 2 intracellular signaling proteins, named GO and TIGERS, interact with each other when cells are treated with an extracellular source of CAMP. The two proteins were tagged with CFP or YFP (CFP = cyan fluorescent protein; YFP = yellow fluorescent protein). CFP is excited by 435 nm light and emits lights at 480 nm. YFP is excited by 480 nm light and emits light at 535 nm. 400 500 Wavelength of Emitted Light 400 500 Wavelength of Emitted Light 600 600 Fluorescence 400 400 500 Wavelength of Emitted Light 600 500 Wavelength of Emitted Light 600 Fluorescence Intensity 400 500 Wavelength of Emitted Light 600 Figure Legend. A. Cells expressing GO-CFP irradiate with 435 nm light. B. Cells expressing TIGERS-YFP and irradiated with 435 nm light. C. Cells expressing TIGERS- YFP and irradiated with 480 nm light. D. Cells expressing GO-CFP and TIGERS-YFP and irradiated with 435 nm light. E. Cells expressing GO-CFP and TIGERS-YFP treated with CAMP and irradiated with 435 nm light.
Figure Legend. A. Cells expressing GO-CFP
irradiate with 435 nm light. B. Cells
expressing TIGERS-YFP and irradiated with
435 nm light. C. Cells expressing TIGERS-
YFP and irradiated with 480 nm light. D.
Cells expressing GO-CFP and TIGERS-YFP
and irradiated with 435 nm light. E. Cells
expressing GO-CFP and TIGERS-YFP treated
with CAMP and irradiated with 435 nm
light.
Question:
The data suggests:
GO and TIGERS do interact in the presence
of CAMP
O It is not possible to tell if GO and TIGERS
interact in the presence of cAMP with the
data provided
GO and TIGERS do not interact in the
presence of CAMP
Transcribed Image Text:Figure Legend. A. Cells expressing GO-CFP irradiate with 435 nm light. B. Cells expressing TIGERS-YFP and irradiated with 435 nm light. C. Cells expressing TIGERS- YFP and irradiated with 480 nm light. D. Cells expressing GO-CFP and TIGERS-YFP and irradiated with 435 nm light. E. Cells expressing GO-CFP and TIGERS-YFP treated with CAMP and irradiated with 435 nm light. Question: The data suggests: GO and TIGERS do interact in the presence of CAMP O It is not possible to tell if GO and TIGERS interact in the presence of cAMP with the data provided GO and TIGERS do not interact in the presence of CAMP
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