You are working with E. coli, studying its metabolism. Your normal lab strain of E. coli can grow using any 6-carbon sugar (glucose, fructose, etc) as its sole carbon and energy source. Recently you have generated a mutant of your E. coll that cannot grow on fructose as its sole carbon and energy source. It can still grow using glucose or other 6-carbon sugars as a sole carbon and energy source. What is the most likely hypothesis for what single gene has been mutated in your new E. coli strain? The fructose transporter Phosphoglucose isomerase (converts glucose 6p to fructose 6pl Phosphofructokinase (converts Fructose-6p to fructose 1.6pl The glucose transporter

You are working with E. coli, studying its metabolism. Your normal lab strain of E. coli can grow using any 6-carbon sugar (glucose, fructose, etc) as its sole carbon and energy source. Recently you have generated a mutant of your E. coll that cannot grow on fructose as its sole carbon and energy source. It can still grow using glucose or other 6-carbon sugars as a sole carbon and energy source. What is the most likely hypothesis for what single gene has been mutated in your new E. coli strain? The fructose transporter Phosphoglucose isomerase (converts glucose 6p to fructose 6pl Phosphofructokinase (converts Fructose-6p to fructose 1.6pl The glucose transporter

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter15: From Dna To Protein

Section15.1: The Connection Between Dna, Rna, And Protein

Problem 1SB

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We isolate DNA from a bacteria that is wild type for the genes arg leu and his shear it in a blender mix it into a culture of bacteria that is mutant for the genes arg leu and his . We then plate on various media. These are the results: Media with arginine 20 colonies /ml Media with histidine 300 colonies /ml Media with leucine 500 colonies /ml Media with all three 10000 colonies/ml 21. What is this experiment called? A. transpiration B. conjugation C. transformation D. transduction
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Recombinant protein production by a genetically modified Escherichia coli strain is proportional to cell growth. Ammonia is used as a nitrogen source for aerobic glucose respiration. The recombinant protein has the general formula CH1,55O0,31N0,25, while that of the cellular biomass is CH1,77O0,49N0,24. The biomass yield from glucose equals 0.50 g/g, while the recombinant protein yield from glucose corresponds to 20% of the cell yield from substrate.a) How much ammonia is required? What is the oxygen demand? (b) If the biomass yield remains the same, what are the ammonia and oxygen requirements for a wild-type strain of E. coli, with cell biomass of the same elemental composition, but unable to synthesize the recombinant protein? (c) On an industrial scale, cultivation takes place in a continuous fermenter at 28°C and the desired recombinant protein production rate is 7 g/h. Since the viscosity of the culture broth is considerable, the energy input due to agitation cannot be neglected.…
Suppose that you are given a short fragment of DNA to sequence. You amplify the fragment with PCR and set up a series of four dideoxy reactions. You then separate the products of the reactions by gel electrophoresis and obtain the following banding pattern:
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Unlike genetically modified organisms (GMOs), synthetic organic substances have not met too much opposition, although the production of both require human intervention and manipulation of natural substances. Why do you think the synthesizing of organic substances is readily accepted by society in general whereas the mention of GMOs immediately raises concerns?