You have a precursor protein that is translated in the cytosol with an N-terminal mitochondrial localization signal (MLS). a) Where will this protein go? b)You perform organelle isolation in cultured cells and compare this protein’s levels in the cytosol and the mitochondria. You perform a western blot which separates the proteins based on their size. Using the same antibody that recognizes this protein you notice that there is a difference in the protein’s size in the cytosol vs mitochondria. How will you explain this?

You have a precursor protein that is translated in the cytosol with an N-terminal mitochondrial localization signal (MLS). a) Where will this protein go? b)You perform organelle isolation in cultured cells and compare this protein’s levels in the cytosol and the mitochondria. You perform a western blot which separates the proteins based on their size. Using the same antibody that recognizes this protein you notice that there is a difference in the protein’s size in the cytosol vs mitochondria. How will you explain this?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter15: From Dna To Protein

Section15.1: The Connection Between Dna, Rna, And Protein

Problem 1SB

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