Chemical equilibrium

substance act like a catalysts, it bind with substrate lower the activation energy to increase the rate of chemical reaction of the cell. Substrate act as a key to enzyme, they will find a enzyme that could bind with them and cause reaction. Active site is where enzyme and substrate bind in together. Activation energy is energy create by combining enzyme and substrate to undergo an specific chemical reaction. Protein is made of one or more lone chain of amino acid. Rate of reaction is

An experiment was done to investigate the effects of low pH on enzymes activities. According to Dunaway, Mariano, D. (2008) enzymes are protein in nature. They are used to speed up the rate of reactions. At, the end of the experiment, enzyme is not used up. There are several factors that affect the rate of enzymatic activities. Some of them include temperatures, pH and amount of reagent. In our experiment, we decided to carry a test on the effects of low pH on enzyme activities. The objectives of

was to observe what the normal enzyme1 (proteins that catalyze chemical reactions) activity of beef liver catalase was, and to measure the effects of extreme changes in temperature and pH on the beef liver catalase function. We also observed what the bromelain1 (proteins in pineapple) activity was in a fresh pineapple compared to canned pineapple. Enzymes are present in all living things.1 These specialized proteins speed up chemical reactions fast enough to sustain life.1 This lab was driven by

Enzyme reactions are an important part of any chemical reaction, but any environmental factors that come can cause it to a great change in the reaction. The purpose for this experiment was to see what concentration of NaCl would cause the greatest change of reaction in lactase (enzyme) activity and what concentration would be the greatest change. In order to observe and analyze the change in enzyme activity a spectrophotometer was used to observe the absorption in a 420nm wavelength to look for the

continue, substrate concentration had to increase. Introduction To understand how and why the experiment was performed, one must understand what enzymes and substrates are. Enzymes are defined as proteins that are capable of speeding up a chemical reaction by reducing the amount of activation energy needed to catalyze that reaction (Raven, Johnson and Mason 2014). Enzymes regulate these biochemical processes

Introduction An enzyme is a protein created by an organism that increases the rate at which chemical reactions occur. There are three different types of enzymes; digestive, metabolic, and food. They break down the vitamins and nutrients that are ingested. Enzymes are organic compounds that are composed of amino acids. They are also facilitators by helping reactants interact to form products in a chemical reaction. They

proper setting of time of experiment, making sure to wait thirty seconds before starting the Lab quest timer, and proper documentation of the graph. To fully understand enzymes, we must first understand how enzymes work. Enzymes speed up a cell’s chemical reactions by lowering energy barriers.

On May 4th, I worked on an experiment to speed up enzyme reactions. Three main things that affect the enzyme reactions are temperature, pH, and shape. For the temperature change, we used a mariabath which will heat up the beaker indirectly. This causes the reactions to speed up, but won’t make the solution go over its boiling point. As the experiment went along, various things were noticed in the enzyme mixtures. The materials used in this experiment included: starch, iodine, water, a glass rod

How Enzymes Work In Different Environments By Sarah Smith Biology1111 October 20, 2011 Lab Partner: Nellie Greer ABSTRACT Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, H2O2, into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this, we added different levels of pH, low, medium, and high, into different test tubes with the enzyme and H2O2,

With both the stock substrate and varying enzyme solutions prepared, the Spec20 spectrophotometer was used to investigate the enzymatic activity of β-Galactosidase through an absorbance-based assay. Using LoggerPro software on the computer to analyze the absorption data, the Spec20 was calibrated before each run with 0.5 mL of the tested enzyme concentration at an absorbance of 420 nm. Data collection was then started, instantly followed by the addition of 0.5 mL of the stock 2.5 mM substrate solution