The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).
The reaction is carried out in saturated aqueous ammonium chloride solution. Thus no special drying of solvents, reagents, or glassware is required. The reaction mechanism for this experiment can be seen below (Fig. 2)
This is done through a change in temperature. Over time, two objects that are in direct contact will
In this experiment, the pKa, dissociation constant, of 2-naphthol was determined by measuring the UV-visible absorption spectra of solution of the acid at different pH values.
Procedure- The procedure for this lab includes many simple steps and a few different things we are testing. Our first Procedure was to combine water and salt to see what kind of reaction it would make. First we fill the graduated cylinder with 100 ml of water. We then measured 1.0 grams of table salt on a balance to get an accurate amount. After, we took the measured amount of salt and poured it into the water filled beaker. Lastly, we watched and recorded the reaction.
During this investigation the question we looked to answer was: what are the identities of the unknown compounds? In an effort to answer the question, our group designed a method in which the relationships between the unknown compounds in plastic bags were compared so that their identities could be determined. The mole (mol) provides a measure of the number of atoms present in the sample of a compound. One mole of an element or compound contains
Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.
Station one: Station one shows that when a small piece of magnesium metal is placed over the flame of the Bunsen burner, the magnesium metal ignites, giving off a bright, white light. Once the flame has extinguished itself due to the lack of fuel, the magnesium metal turned from a metallic strip to a crumbled white powder. It can be decided that station one is a combination (redox) reaction, as a new product is synthesised by the combination of two reactants. The reactants, magnesium and oxygen gas in the air bonded together to form the product of magnesium oxide.
Water uptake capacity of NCs enables them to entrap exudates upon contact with suppurating wounds which is desirable for their effectiveness as wound dressings. The increase in size and agglomeration of AgNPs from NC-1 to NC-3 might have resulted in more blockages of pores of CNCs which could be responsible for a decrease in water uptake capacity of NC-2 and NC-3 as compared to NC-1.
The N2O and NO fluxes measurements were conducted in 2–28 days intervals during the growing season and 10-30 days intervals during the non-growing season and between 8:00 and 11:00 h each measuring day to minimize the effect of diurnal temperature variation. The stainless steel chamber (40 cm in diameter and 30 cm high) was used for the measurement (Toma and Hatano, 2007). Gas samples were taken from the chambers using a 50 mL syringe. The samples N2O were collected for 0; 15 and 30 minutes and for 0 and 30 minutes for NO samples. N2O was analysed using gas chromatography with ECD (GC-2014 Model, Shimadzu) and NO using NOx Analyser (265 P Model, Kimoto Electric, Osaka, Japan). Gas flux is the gradient of gas concentration in chamber over time. The cumulative gas ﬂux was calculated by
The mean voltage of the battery terminals while connected to the identification resistors is presented in Figure 4 12. These samples have been pulled out from the voltage sensor of the PEB panel. The voltage decreased as expected from 12.53 to 12.5 during first 20 seconds of connection to the
The light yellow precipitate was collected by suction filtration using a Hirsch funnel. The product was washed with two 1-mL portions of cold methanol followed by two 1-mL portions of diethyl ether. The product was dried in the oven at 110°C. The IR spectrum as a KBr pellet was obtained for the product and inosine for analysis.
Abstract: In this lab report two unknown substances will be tested. The tests performed were looking for the presence of the main biomolecules, Carbohydrates, Lipids, and Proteins. The tests used will be used in the Lab report are; The Benedicts test to test for sugars, the Iodine test for coiled or non-coiled carbs, The Grease-spot test for lipids, and the Biuret test for proteins. Tests show that both substances had different responses to the Benedicts test. Both substances shared the same response to the Iodine test. In the Grease-spot test both showed the same. In the Biuret test the unknown substances resulted differently from each other.
This lab report identifies gas produced by chemical reactions and tests for oxygen, hydrogen or carbon dioxide.
1-Vinylimidazole (VIm), 1-idobutane (IB), 2-aminoethanethiol (AET), acetylacetate, N,N'-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), posstasium carbonate (K2CO3), and azobisisobutyronitrile (AIBN) were purchased from Sigma-Aldrich (Milwaukee, WI, USA). 4,4′-Bis(4-hydroxylphenyl) valeic acid (HPV), 4,4′-difluorobenzophenone (DBP), chloroform, sodium hydroxide (NaOH), dimethylacetamide (DMAc), dimethyl sulfoxide (DMSO), toluene, hydrochloric acid (HCl), tetrahydrofuran (THF), iso-propanol (IPA), and dimethylformamide (DMF) were purchased from TCI company (Tokyo, Japan).