4-Nitro-O-Phenamine Lab Report

Water uptake capacity of NCs enables them to entrap exudates upon contact with suppurating wounds which is desirable for their effectiveness as wound dressings. The increase in size and agglomeration of AgNPs from NC-1 to NC-3 might have resulted in more blockages of pores of CNCs which could be responsible for a decrease in water uptake capacity of NC-2 and NC-3 as compared to NC-1.

The N2O and NO fluxes measurements were conducted in 2–28 days intervals during the growing season and 10-30 days intervals during the non-growing season and between 8:00 and 11:00 h each measuring day to minimize the effect of diurnal temperature variation. The stainless steel chamber (40 cm in diameter and 30 cm high) was used for the measurement (Toma and Hatano, 2007). Gas samples were taken from the chambers using a 50 mL syringe. The samples N2O were collected for 0; 15 and 30 minutes and for 0 and 30 minutes for NO samples. N2O was analysed using gas chromatography with ECD (GC-2014 Model, Shimadzu) and NO using NOx Analyser (265 P Model, Kimoto Electric, Osaka, Japan). Gas flux is the gradient of gas concentration in chamber over time. The cumulative gas flux was calculated by

Data Table 2 shows the results gathered after the purification of 3-nitrochalcone. Since only half of the crude product was used, the theoretical yield for the crystallized product was determined to be 2.1 g. This led to a percent yield of 39%. The melting point of the crystallized product was also determined to be 142℃, which differed slightly from the literature value of 146℃. As a result, the product, 3-nitrochalcone, was not completely pure. It probably contained water and alcohol, because any impurities would have lowered the melting point. Considering the fact that the crude product was not thoroughly dried before purification, water and ethanol were already in the product. Even after crystallization into “pure 3-nitrochalcone”, the product was also not completely dried. The yield should have been much lower than 39%. If the product is not allowed time to dry, not only water, but methanol, which was the solvent that was used to crystallize the crude, would be in the product. Both the crude and crystallized 3-nitrochalcone were analyzed by infrared spectroscopy to test and prove these

where a plot of ΛM versus √C gives a straight line of an intercept equal (Λ0) and a slope equal to the Onsager constant. In our solutions, the following equilibria are deliberated: M+ + A− ⇆ MA

The reaction is carried out in saturated aqueous ammonium chloride solution. Thus no special drying of solvents, reagents, or glassware is required. The reaction mechanism for this experiment can be seen below (Fig. 2)

When calcium chloride, sodium bicarbonate, and phenol red are put into a bag and mixed up the type of reaction that occurs is a chemical reaction for the following reasons. Things that indicate that a chemical reaction has occurred are color change, formation of precipitate, formation of gas, and energy transfer in which three of them took place in the reaction of calcium chloride, sodium bicarbonate, and phenol red. The three indicators in this experiment were: a color change, formation of gas, and an energy transfer

During this investigation the question we looked to answer was: what are the identities of the unknown compounds? In an effort to answer the question, our group designed a method in which the relationships between the unknown compounds in plastic bags were compared so that their identities could be determined. The mole (mol) provides a measure of the number of atoms present in the sample of a compound. One mole of an element or compound contains

Following the instructions from the Biofuel Enzyme Kit, we performed a baseline experiment in which all factors remained constant except time in order to establish a control for absorbance and concentrations of p-nitrophenol. We then used this information to determine the Vo and the other concentrations in order to form a graph. On a molecular level, since the temperature, pH, inhibitor,

In this experiment, the pKa, dissociation constant, of 2-naphthol was determined by measuring the UV-visible absorption spectra of solution of the acid at different pH values.

Distilled water changed the color of copper (II) nitrate into a lighter shade of blue and diluted the solution.

Nitric Oxide is essential for penile erection. Erectile Dysfunction (ED) is associated with reduced NO synthesis or reduced release in the erectile tissue. The isoforms of NOS, eNOS and nNOS mediate penile erection. These isoforms are regulated by NOS cofactors, calcium, calcium binding protein calmodulin, oxygen and reduced NADPH. After the increase in intercellular calcium production, NO is generated in low amounts. Upon neural depolarization in nNOS containing nerve terminal in penis, neural NO is released. Similarly, upon local neurogenic stimulation, nerves in contact with the endothelium, eNOS is liberated. “Shear Stress,” the mechanical forces of blood flow on the vascular lining also releases NO from eNOS. Further investigation also

The first portion of the lab was about preparing the standard curve for the reaction products. In this part we already know the concentration of the product, Nitrophenol. So, to perform this we took six test-tubes and filled each with 1ml of standard labeled with S1-S6 respectively. For example 1st =S1, 2nd=S2, 3rd =S3 and so on. After that we measured the absorbance of each test tubes setting the first one as blank. And the data is recorded in the table (5.1). After recording the reading we constructed a Nitrophenol Standard curve by plotting A410 on y-axis and concentration on the x-axis as on figure (5.2). The second portion require us to prepare acid phosphatase from the wheat germ extract. But, this part of the lab was already

A chemical reaction is a process that involves rearrangement of the molecular structure of a substance. Once this happens, you will not be able to reverse the chemical change. To observe the factors that affect the reaction, an experiment was performed on CaCO3 to explore the many states of matter. Kinetic theory is the first topic to explore. Kinetic theory is the amount of energy each state has. For example, solids have little kinetic energy and gas has the most. The denser the substance is, the less kinetic energy it has. All matter is made up of atoms and molecules that are continuously in motion. When heat is added, the particles vibrate faster and take up a larger surface area however the mass of the substance stays the same no matter

The influence of changing amino acids sequence to the Alkaline Phosphatase (AP) enzyme optimal functions

Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.