50 Germinating Wheat Seedlings

In this experiment, my hypothesis was not supported. The hypothesis stated that acid rain would decrease the rate at which seeds germinate. The hypothesis is rejected due to the data which shows that the average sprout length of the control group is shorter than the experimental group. Although the experimental group grew taller and faster, the control group looked healthier. The control group’s sprouts were greener and stood up taller while the experimental group’s sprouts were a very milky-white green and leaned over some.

In this lab we are measuring the amount of oxygen used in both germinating and non germinating peas. We are measuring the oxygen consumption by taking a reading of a respirometer submerged in two water baths. The first bath will be cold water and the second warm to determine the effect of temperatures on oxygen consumption. Our negative control will be glass beads to measure to increase or decrease in atmospheric pressure or temperature changes. There is a direct relationship between oxygen consumption and Carbon Dioxide produced, therefore the more O2 consumed the more CO2 produced. To keep the amount of CO2 produced from canceling out any pressure gained or lost from the consumption of

For my seed experiment I had decided to see what the effect of sprinkling salt on a radish seed would be. So for my control group I had set six (6) cherry radish seeds in between a damp paper towel and then closed it within a Ziploc bag. For my experimental I had set it up the exact same way as the control group but I would sprinkle salt on top of seeds before I zipped up the baggie. I sprinkled the salt on the experimental seeds and dampened the paper towel once every day. Each bag was stored in the light and at room temperature (~70° F.)

This experiment consisted of 3 respirometers, one with ants, one with radish seeds, and one with glass beads. Each with 4 pellets of KOH and a piece of cotton. They were placed in a water bath that was at 75 degrees fahrenheit. A bubble at the end of the respirometer was measured every five minutes, and this distance showed how well the organisms were respiring. The radish seeds were able to do the most cellular respiration in 25 minutes, with the ants being a close second, and the control respirometer of the glass beads doing the least.

We utilized two sensors, which are the Carbon Dioxide (CO2) Sensor and the Oxygen (O2) Sensor, and a Chamber, which is where we are going to add the beans and the two chambers. The objective of this experiment is to see the production rate of oxygen and carbon dioxide within the germinated Beans, which there is 3 species (Pinto Beans, Lima Beans, & Chickpeas) and to compare them. We got that most of them are doing Cell Respiration, since most of them have Carbon Dioxide production increasing, while Oxygen production is decreasing, or increasing, but barely. Most of these were affected by temperature, which affects the mitochondria, also placement of sensors in the chamber, etc. And comparing to all the results of the samples of each bean, CO2

The experiment’s variables include the independent variable, which is the concentration of gibberellic acid that is used to soak the seeds, and the time period of germination, as well as the dependant variable, which is the number of seeds that germinate. The control used to compare seed germination and in this situation is the sample with a gibberellic acid concentration of 0 ppm. Constants in the experiment include factors affecting seed germination such as constant temperatures where each sample receives the same temperature and in the case of variation the same amount of variation, quantity of moisture where the same amount of gibberellic acid is added to each bag corresponding to the concentration, the samples should also receive the same conditions of light, have the same sowing density, and number of seeds per square centimetre.

When carrying this experiment the fundamental purpose was to explain fermentation which is a catabolic process that makes a limited amount of ATP from glucose (Urry, Cain, Wasserman, Minorsky, & Reece) by adding yeast to 3 different carbohydrate solutions used as a food sources. We used 4 different test tubes containing glucose, sucrose, saturated starch and water. The purpose was to see which carbohydrate would ferment the most in various temperatures.The levels of CO2 were tracked over a period of time. According to our predictions we believed from slowest to fastest at 4 degrees water, saturated starch, glucose and lastly sucrose. At 23 degrees water, saturated starch, glucose and lastly sucrose. Finally at 37 degrees water, saturated starch, glucose and making sucrose the fastest. We believed this because sucrose would dissolve faster therefore creating faster CO2 levels. On the other hand the Cellular respiration of breakdown organic molecules that use electron transport chain that produce ATP, we had to find out how increasing succinate in DPIP which is an electron acceptor that will intercept the hydrogen ions released from succinate. (Upadhyay, 2017) would allow an accelerated reaction to occur. We believed 3 would be the highest because it included every type of reduce reactions and 1 would be the slowest since succinate wasn't involved. Based on our data collected we came to a conclusion that glucose the carbohydrates that fermentation had the highest rate and most efficient food source. Due to the temperature being so high it produced more gas. Regarding the

Since this lab had two parts to this experiment, it took two weeks to complete it. For this experiment, pages 65-73 in the East Tennessee State University BIOL 1111 Lab book were used for this lab. The first week we measured the rate of respiration in Experiment A. 10mL of yeast suspension was placed into each of the 7 large diameter test tubes. Then after that was completed, six of the seven tubes were filled with a different type of sugar: glucose, fructose, lactose, galactose, sucrose, honey, and the 7th tube were filled with water for the control of this experiment. The tubes were filled approximately ¼ inch below the rim of the tube according to the lab book. After this procedure was completed, all the seven tubes were capped off with

3.Secure the other end of the tube into the hole located on the rubber stopper

The research question asks how varying sucrose concentrations affect the rate of anaerobic cell respiration in yeast, measured in CO2 production. The rate of anaerobic respiration will be determined by measuring the rate of CO2 production by the yeast cells.

These are used to measure the amount of O2 that was produced in the chamber. Also needed are five different colored lights. The colors used in this experiment were blue, green, orange, red and white. Each light produces a different wavelength and thus a different rate of O2 production. The plant that was tested is called Elodea; this plant should be cut to 10 cm and placed into the respiration chamber and then covered with tin foil and sealed with the O2 sensor.

ABSTRACT The experiment performed was designed and processed to determine if yeast cells are able to undergo fermentation with other food molecules when enclosed in a fermentation tube with no oxygen. The yeast and food molecules were mixed together evenly and allowed to incubate for approximately one hour. The tests were then carried out to determine if respiration (anaerobic and aerobic) took place within the fermentation tubes. If there was any detection of carbon dioxide then that is an indicator of a respiration taking place.

The relationship between the celery, water temperature, and capillary action in the celery is the transpiration, adhesion, cohesion, osmosis, and respiration processes. Respiration is related to this experiment because when the water evaporates from the leaves(transpiration), it pulls water from the tubes of the celery, called xylems, and causes the water to rise, as well as the capillary action causes the rising. Water molecules are attached to each other through cohesion, so they don’t want to “let go” of the other water molecules they/ it is attached to. We saw evidence of this when we cut down the celery in small slivers until we saw the dye that had risen up the tube.

In this experiment, seeds were using oxygen for respiration, so the level of gas in the respirometer decreased. This is why the level of oxygen in the pipettes went down. This happened because of respiration, where cells take in oxygen to make ATP and release carbon dioxide. The carbon dioxide made was absorbed by potassium hydroxide. With the data, the germinating seeds used oxygen, and so did the rest, strangely. The relationship between oxygen consumption and time was an increasing one, so as time passed, more oxygen was used, as shown back in figure 1. Germination causes cellular respiration to occur and though germinating seeds should have been the one who respired most by far, the data collected said otherwise, and this could have been

The environmental factor tested in this experiment was the addition of fertilizer versus just water giving water alone. It was hypothesized that if seeds were given fertilizer then they would germinate faster and their stems and roots would grow longer than the seeds that were given water only.