Agarose Vs Polyacrylamide

The idea behind Gel Electrophoresis is that we inject a slab of gel with the DNA we found at the crime scene. We then inject the same gel, next to the crime scene DNA, with suspect 1’s DNA and suspect 2’s DNA. We then send an electric current through the gel and wait for the results. The smaller molecules in the DNA will travel farther than the bigger molecules because the bigger ones will have difficulty making its way through the microscopic beads in the gel. After the separate bands appear in the gel, we stain it with a special chemical called Ethidium Bromide to give it a color under the blue

We placed the gel into the running chamber, and then we completely covered the gel with TAE. 3 microliters of loading dye was added to each tube; this would help distinguish the enzyme from the gel. As before, we tapped the tube on the table to mix. Then we carefully added each of the four samples into their own wells. A total of 33 microliters of each sample was poured into each well. Afterwards, we attached the positive and negative electrodes to their corresponding terminals on the power supply and gel box. We turned on the power to around 80 volts and waited 45-60 minutes for the loading dye to move down the gel approximately 6-8 cm. Finally, we were able to visualize the DNA in the gel and write down the

Using the electric current, scientists pass the DNA through gel, and as smaller molecules get through gel quicker than those of bigger sizes, DNA molecules get separated according the sizes of the molecules. We utilize the property that large molecules move slower, and DNA is slightly negatively charged(due to phosphate groups), so it will move to the positive pole of the gel.

Figure 1 Gel Electrophoresis for Replication Taster PTC. The gel is composed of an ethidium bromide stained 3% agarose gel demonstrating DNA fragments which were a depiction of PCR amplification. The agarose gel contains nine loading samples, including from left to right, the MW marker lane 1 precision mol mass standard, lane 2 TB undigested PTC (5µl of DNA, 5µl of master mix P, and 2.5µl of loading dye), lane 3 TB digested PTC (5µl of DNA, 5µl of master mix P, 2µl Fnu4HI, and 3µl of loading dye), lane 4 TB A(L)DH G (10µl DNA, 10µl master mix G, and 5µl loading dye), lane 5 TB A(L)DH A (10µl DNA, 10µl master mix A, and 5µl loading dye), lane 6 MG undigested PTC (5µl of DNA, 5µl of master mix P, and 2.5µl of loading dye), lane 7 MG digested PTC (5µl of DNA, 5µl of master mix P, 2µl Fnu4HI, and 3µl of loading dye), lane 8 MG A(L)DH G (10µl DNA, 10µl master mix G, and 5µl loading dye), lane 9 MG A(L)DH A (10µl DNA, 10µl master mix A, and 5µl loading dye).

Gel electrophoresis is a procedure used in laboratories to separate DNA, as well as RNA and proteins. A gel slab is placed in a buffer-filled box and an electrical field is applied. The negatively charged DNA will migrate towards the positively charged side, where it can then be recorded and further analyzed.

Throughout analysis of the data, we complete DNA agarose gel electrophoresis in order to determine the size of our plasmid DNA. The electrophoresis

This experiment’s purpose is to identify and separate DNA macromolecules with a homemade gel and electrophoresis chamber. After separating DNA with a homemade gel and electrophoresis chamber, it must be compared to the regular gel electrophoresis chamber. In order to create a homemade gel electrophoresis chamber, five 9-volt batteries were connected and attached to stainless steel wire which were submerged in the 20x buffer as well as the gel. The two wires acted as the positive and negative electrodes as seen in a gel electrophoresis chamber. After the experiment was done, the homemade gel electrophoresis chamber was proven to mock the regularly used electrophoresis chamber. The experiment suggested that the results would not change if the

In slab gel electrophoresis agarose or polyacrylamide is used. The conducting buffer is contained in the porous gel and the gel is then poured in between glass plates. These plates are separated by spacers. An electric field is applied at the rear after the substance of experimentation is added to the wells at the top. The substance will then move a certain distance towards the positive electrode. This method uses gel unlike the cellulose acetate strips but it still uses a buffer and a electric field to migrate the substances based on their size and charge. Slab gel electrophoresis is usually used in the biological

Our materials for the extraction of DNA included: the screwcap tubes, capless tubes, pipet tips, disposable transfer pipets, a foam microtube holder, a DI water bottle, a sharpie to label, a hot water bath, Non-GMO food, Test food, and a centrifuge. Our materials for the setup of the PCR reactions included: PCR tubes, screwcap tubes, pipet tips, an aerosol barrier, a foam microtube holder, a PCR tube holder, a 2-20 microliter micropipette, an ice bath, a sharpie to label, a plastic bag, a styrofoam cup with ice water, and a black tray with ice water. Finally, the materials for the electrophoresis of the PCR products included: Agarose gel, PCR sample tubes from previous labs, PCR tube holders, running buffers (300-350 ml), loading dye, a PCR molecular weight

Analysis of DNA from practicals 1 and 2 using the technique of agarose gel electrophoresis and analysis of transfomed E. coli from practical 2 (part B)

Electrophoresis can be used to get DNA fingerprint for forensic purposes or to test genes for a particular disease(Gel electrophoresis).The purpose of gel electrophoresis is to picture, identify and distinguish between the molecules that have been processed by other methods such as PCR or enzymatic digestion. Different methods are run through the gel electrophoresis to differentiate between molecules (Gel Electrophoresis - Definition, Purpose and

The last figure, Figure 4, represents the electrophoresis of the fragmented DNA from the beginning experiment after all of the necessary steps were taken. Upon interpretation one may notice two bands, one upper and one lower. The

Gel electrophoresis is the method of separating and observing macromolecules (DNA, RNA, and proteins) and their fragments. The conditions that allow macromolecules to separate during gel electrophoresis include their charges and sizes. Restriction enzymes split DNA molecules in certain places. In gel electrophoresis, an electric current moves DNA molecules across a slab of agarose gel or a polysaccharide medium, and the polysaccharide medium or the medium with sugar molecules then withholds the DNA molecules as they are conveyed by electric currents. The phosphate groups in DNA molecules’ backbone possess a high negative charge. When DNA molecules are put into a field of electric charge, the high negative charge of DNA molecules force the molecules to run to the positive or opposite end of the agarose gel. Because agarose gel contains small molecules of sugar molecules, large molecules of DNA struggle migrating towards the positive end of the agarose gel because of their bulkiness. Whereas small molecules of DNA have little problem moving or move faster towards the positive end of the agarose mesh because they do not bump into sugar molecules as often as large DNA molecules do. What is more, the main setup of gel electrophoresis is that the gel will be submerged a in buffer

Gel electrophoresis is “a method that separates macromolecules,” (Reece et al. 243) using a gel plate and an electrical charge. A gel plate is a rectangle of “jellylike material often made from agarose,” (Reece et al. 243). It was developed by Oliver Smithies in 1950 because scientists were in need of a faster and less expensive way to compare DNA samples. Although scientists were hesitant of this technique at first, gel electrophoresis was proven to be an effective process. Later in 1975, scientist Fred Sanger improved and refined the steps of gel electrophoresis, making it cheaper and more efficient. Sanger was granted the Nobel Prize for his work in 1980.

were placed into the gel electrophoresis aparatus where we were able to see if the sample of DNA