Analysis : Like Cat And Apx, Pod ( Guaiacol Peroxidase ) Activity

In this experiment, the naturally occurring peroxidase is extracted from homogenized turnip (Brassica rapa) pulp (Coleman 2016). Its role in the environment is to remove toxic hydrogen peroxide during metabolic processes where oxygen is used (Coleman 2016). The goal of this experiment is to evaluate the change of absorbency of turnip peroxidase within a metabolic reaction utilizing oxygen. Any change noted is indicative of the peroxidase removing hydrogen peroxide. Within this experiment, the extract will be prepared, the amount of enzyme will be standardized, and the effect of changing the optimal conditions will be observed. If the enzyme concentration is increased then the rate of the reaction decrease. If the pH of solutions used is increased

Our data recorded shows that the germinating peas did consume more oxygen than the non-germinating or the glass beads alone and that the cooler temperature did slow down the consumption of oxygen in the germinating peas. In both water baths the atmospheric pressure seemed to increase causing our reading to raise in our glass beads and non-germinating peas. This direct relationship in reading leads us to believe that the oxygen consumption in the non-germinating peas was minimal if any at all.

The Impact of Temperature and pH on the Enzyme Activity of Catechol Oxidase in Solanum Tuberosum Samples

The use of too much fertilizers, and in particular, of fertilizers with high concentrations of nitrogen, has been linked to reduced biodiversity (Xiankai et al. 2010). It becomes necessary to consider the detrimental effects of high concentrations of fertilizer in the reduced spaces. Fertilizers are salts, and therefore high concentrations of fertilizers can deplete the plant from water.

Throughout this experiment, we are researching the effect on the growth and survival of Wisconsin Fast Plants using fertilizer pellets to help with the growth of the plants. Wisconsin Fast Plants is a plant member of the crucifer family which is related to other plants (vegetables) such as cabbage, broccoli, turnips, etc. This plants are small and can grow very easily because they go through their cell cycle around 40 days. Wisconsin Fast Plants Fertilizers are different materials used that can provide plants with the nutrients it need to grow. (1) These plants are a good model system to study because they grew very quickly and didn’t need a lot of resources to grow making them the perfect plant to use for studies. (4) By using the fertilizers,

Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, H2O2, into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this, we added different levels of pH, low, medium, and high, into different test tubes with the enzyme and H2O2, and we then inverted the tube. The amount of O2 gas produced was then measured and recorded. The result was that the higher pH produced more gas, followed by medium pH, then low pH. The enzymes were more active in the pH of about 10. It increased

The pH of soil is important for the absorption of nutrients into the plant. Of the 17 needed plant nutrients 14 of them are acquired through the soil. Acidity is needed to break down and dissolve these nutrients. The nutrients are able to dissolve into the soil faster when the acid is acting as a solute. Another way the pH affects the soil is by influencing microorganisms. The bacteria is crucial in the growth and development of the plant, the bacteria’s role is to break down and decompose organic matter in the soil. If the pH of the soil is too high the acid will slow down and eventually stop the microorganisms. Most plants ideal pH is between 6-7, slightly acidic. Many plants are outliers and thrive in pH such as carrots and corn, which can withstand pH as low as 5.5. If the pH of the soil is too high for the desired crop farmers can add material such as limestone, and wood ashes to raise the pH to the desired level. The pH of the soil can also be changed naturally through the leaching of calcium, magnesium and sodium by rainwater. Carbon dioxide from rotting organic matter can also increase the pH of the soil. Acids can also be created organically in the form of sulfuric and nitric

The purpose of this lab was to investigate and observe the effects of organic vs. synthetic fertilizers on plant growth by planting lima beans with added amounts of fertilizers, and to see how does adding different nutrients to the soil affect the growth of the lima bean? A significant difference was examined between the plants that contained manure and miracle growth, unfortunately the plant with no additional fertilizers (Plant #3) did not show any growth. The plant that grew the most was the one that contained manure, to an extent the one that grew the most in a short period of time was the one that contained miracle growth, as shown in figure I. The hypothesis explaining if the Lima Bean plant contains synthetic nutrients in the soil then

The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen.

How do various factors, pH, enzyme concentration, ionic concentration, substrate concentration, and temperature, effect turnip peroxidase absorbance/ transmittance rate? The initial experiment was testing the effects of pH buffers (3,5,7,9,and 11). If the pH buffers of 3, 5, 7, 9, and 11 are tested on the turnip peroxidase, the enzyme will function best, and the reaction rate will be the fastest, under conditions of a neutral pH because the cytoplasm of most cells has a pH of about seven and usually extreme pH’s in an enzymes environment usually denature the enzyme, restricting it from catalyzing the reaction. First, a baseline was tested to establish a standard control for a reaction to reference to when testing other factors, to

The purpose of this experiment is to learn the effects of a certain enzyme (Peroxidase) concentration, to figure out the temperature and pH effects on Peroxidase activity and the effect of an inhibitor. The procedure includes using pH5, H202, Enzyme Extract, and Guaiacol and calibrating a spectrophotometer to determine the effect of enzyme concentration. As the experiment continues, the same reagents are used with the spectrophotometer to determine the temperature and pH effects on Peroxidase activity. Lastly, to determine the effect of an inhibitor on Peroxidase, an inhibitor is added to the extract. It was found that an increase in enzyme concentration also caused an increase in the reaction rate. The reaction rate of peroxidase increases at 40oC. Peroxidase performed the best under pH5 and declined as it became more basic. The inhibitor (Hydroxy-lamine) caused a decline in the reaction rate. The significance of this experiment is to find the optimal living conditions for Peroxidase. This enzyme is vital because it gets rid of hydrogen peroxide, which is toxic to living environments.

The purpose of this report is to find out the effect of change in the Temperature, PH, boiling, concentration in peroxidase activity. Peroxidase is an enzyme that converts toxic hydrogen peroxide (H2O2) into water and another harmless compound. In this experiment we use, turnips and horseradish roots which are rich in the peroxidase to study the activity of this enzyme. The activity of peroxidase with change in temperature was highest at 320 Celsius and lowest at 40C. The activity of peroxidase was highest at a pH of 7, while it was lowest at pH of 9.Peroxidase activity was very low and constant with boiled extract, while the activity was moderate

will be working at the pH 7 the majority of the time and our bodies

Based on the class data of the pH of phosphorylase reaction, the potato phosphorylase is reached the endpoint which the phosphorylase active at pH 6, it started active within 6 minutes. The optimal pH of phosphorylase is at pH 7 which active just within 4 minutes. At pH 6, it started to breaking down the starch primer +glucose-1-phosphase into starch + phosphate which reacted with the iodine test to formed the blue precipitate. At the optimal pH 7, it shows that it maximized its activity. Comparing the data of the pH of the potato phosphorylase reaction with the study published by Russell Pressey from Plant

The plants that grow in saline soils have diverse ionic compositions and a range in concentrations of dissolved salts (Volkmar et al., 1998). These concentrations fluctuate because of changes in water source, drainage, evapo-transpiration, and solute availability (Volkmar et al., 1998). Due to these varying conditions, plant growth depends on a supply of inorganic nutrients, and this level of nutrients varies in time and space (Maathius and Amtmann, 1999). Either extreme condition concerning nutrients results in deficiency or toxicity in plants, and this is demonstrated by salt tolerance (Maathius and Amtmann, 1999). These conditions vary according to the plant species and growth conditions. Little is known about the genetic basis for diversity of salt tolerance in plants, and this could be partly explained through the definitions given for salinity.