Ap Biology Pond 5 Lab

We did 3 different test to help conclude the water quality. The first testing/station i did was to see what kind of critters were living in the water. Then, my next group was to take a test to see if eutrophication was in the water. We also took

We had to get exactly 25ml of water from the creek, and calculate the PPM of dissolved oxygen in it using a test kit. So, we went down to the creek, got exactly 25ml of water, and then we brought it back. We had to break a dropper in it and watch the colour of the water. We found that the colour matched 12 PPM on the scale; a deep blue. We collaborated with the other groups, and wrote down the other groups’ results to compare. We calculated that the average pH for the creek water was 7; entirely normal. We did some other stuff, like finding the number of rocks that neutralized acid in the creek, and vice

The purpose of this lab was to practice proper handling of the light microscope, learn the names and functions of the light microscope parts, acquire skill in using the light microscope by carefully following all directions, prepare a wet mount, and locate objects under low and high power magnification. In addition, we will learn to position objects when viewed with a microscope, adjust the diaphragm correctly to achieve proper light under low and high power, learn to locate objects at various places in the “depth of field” and use stains (iodine) as an aid.

Two things I learned was from Turtle pond lab was what B.O.D means for the ponds water quality and how to calculate it. To answer question number two I chose to explain the B.O.D results. I didn´t know much about the Biochemical oxygen demand so, I had to do a little bit of research on it. I find out that a high B.O.D measurement is actually bad because it means that the water is polluted and that B.O.D is used to measure a number of dissolved oxygen that an organism needs to break down organic material in a water sample. During this lab, I also learned how to measure BOD. When we were at the pond my job was to take a sample of the water in a black bottle and then 5 days later we would measure the dissolved oxygen in the sample. To find the

The average temperature was approximately 9.67 C. The temperature of the water is significantly impacted by the amount of cool wind the area receives, the amount of sunlight, and the time of year in which the temperature was measured. These factors allow for the water to maintain significantly cooler throughout the year. Also affected by wind are the velocity and rate of flow of the stream. The average velocity is 0.29 meters per second (m/s) and the rate of flow is 41.30 meters3 per second (m3/s). The velocity and rate of flow in the area are higher because it was closer to the dam and also contained areas that were more elevated than some. Sunlight, on the other hand, also affected the turbidity and the photic zone. The turbidity of the stream was 26.50 centimeters (0.265 meters), while the photic zone was 0.42 meters. The photic zone is the depth of the water that receives the greatest amount of sunlight that is then used to carry out photosynthesis. Turbidity is an indicator of how much algae or sediments is present in a lake because it affects the visibility of the water. The cloudiness of the water is caused by the dissolved particles scattering light molecules within the water. The average depth of the water is 9.50 meters and the average width of the water is 14.99 meters. The measurements of the width and depth are not affected by anything. According to Trout

Sediments are the main source of water pollution, contributing to turbidity issues as well as irregular or harmful nitrite/nitrate, phosphorus, and pH levels. This contributes to the death of marine organisms and can also change which organisms can survive in the body of water as its conditions change due to runoff. Anthropogenic runoff is also a contributor of adverse water effects, such as cultural eutrophication from fertilizer runoff, and also results in the death of aquatic animals and shifts in which organisms are more prominent in the ecosystem. This lab will address the effects soil will have on variables concerning water quality. There is also the option of including fish and/or aquatic plants in the water column, which are independent variables as well as the soil. The pH, ammonia levels, nitrite levels, temperature, D.O., and physical attributes are the dependent variables that will be measured during the lab. The qualitative physical tests (turbidity and odor) will portray the physical state and cleanliness of the water, as well as the level of runoff from the soil.

Based on the obtained results. We have a sample solution from the Oak Flat Pond (Surface water) had:

3. Soil samples were collected at varying distances from the lake at 10m increments (0-40m) using the shovel and meter stick. Soil samples were only collected from a depth of about 2-8 inches.

One of the most family-friendly things to do in the area, the Key West Aquarium has delighted visitors of all ages since 1934. Open for business during the Great Depression, the Aquarium was Key West’s first attraction. Today, it remains a popular destination, showcasing marine life indigenous to the Florida

Purpose: The purpose of this experiment is to learn about and use the Scientific Method. The discussion of physical properties such as density, color, texture, smell, and solubility will take place.

The results for DO include 12/3/12: 3.2 Mg/L, 13/3/13 29 Mg/L, 14/3/14 66.73 Mg/L or % saturation? 15/3/15 92% saturation, 15/9/15 85% saturation. I think 66.73 result was % saturation so that is the result I am using. Recordings of Freshwater Creek on the 22nd of February 2016 show results of DO in both Mg/L and % saturation, these results are 6.32 Mg/L and 80% saturation and for air temp 27.13 Degrees Celsius. Resource 4 shows that water and air temperature coincide with the amount of average/normal Mg/L of DO and Degrees Celsius. For 27.13 Degrees Celsius the Mg/L should be around 8, since the result is 6.32 Mg/L this is under normal amount, however it is not at 0 so the water isn’t completely contaminated/polluted. The year 2012 DO was at an extremely low 3.2 Mg/L this could have been a migration of fish such as tilapia which may have caused many fish to die off, however the next year the Mg/L was at 29 which may have been a direct result of either migrant fish killing off other fish or flooding which killed fish. A significant trend to result was the temperature were the temperature only fluctuated by less than 1.5 degree (resource 5) however that trend is broken due to 2016 results of 27.13 Degrees which is an unusual result due to the

The specific aims for Experiment 1 was to study the efficiency of phagocytosis analyzing the macrophages’ abilities to engulf Nile Red beads. We also looked at the impacts of effectors on their abilities and also different concentrations of resolvin. For Experiment 2A, we studied the efficiency of efferocytosis by analyzing the macrophages’ abilities to engulf and digest apoptotic cells under various effectors and concentrations. In Experiment 2B, we studied the efficiency of efferocytosis by analyzing the macrophages’ abilities to engulf and digest apoptotic cells under effectors and cytokines. We hypothesized that having an effector (resolvin D-2 and lipoxin) and a pro-resolving cytokine (IL-13) would increase efferocytosis than having either one alone or having neither one.

results for these lakes, which are in close vicinity to the lakes tested in this experiment,

In each container, I put five fish. In one container, I put a portion of “Wolffia Brasilensis” in, as well as the fish flakes for food. In the control container, I put only the fish flakes. I planned to not change the water for a week to see the maximum discrepancy between the two containers.

Experimental Method: The experimental design was sourced from the lab course manual: Setting-up RNAi With a light microscope, two live worms were located on the control plate. 180 μl of M9 buffer was mixed with 20 μl of 1M IPTG. The entire 200 μl was pipetted into the plate covering the bacteria. The plate sat for ten minutes until the liquid was completely absorbed. The worms were put under the microscope to ensure they were still alive.