Bacteri Bacteria And The Test Tubes

1994 WordsDec 23, 20158 Pages
Bacteria Growth in Different Test Tubes Introduction The purpose of this lab is to learn about restriction enzymes, plasmids, and cloning genes. In class, we learned a lot about the structure of DNA and genes, which were both very helpful while performing this experiment. Restriction enzymes have the ability to cut DNA at specific sequences. Plasmids are perfect for genetic engineering because they can replicate and initiate transcription. Performing this experiment helped us learn more about the process of cloning a gene. We also learned about transcription and translation in class, which are both crucial parts in the process of cloning a gene. It was also necessary that we knew information about gel electrophoresis. In this process, DNA fragments are separated based on their size, electrical charge, and other properties. In biotechnology, living organisms are altered so that they can be more We predicted that in the LB plate, there would be some growth on the P+ and P- sides.We predicted that in the LB/amp plate, there would be a lot of growth on both sides. We also predicted that in the LB/amp/ara plate, there would be a lot of growth. Materials and Methods 2A Reagents Microfuge tube of 2.5x restriction buffer (2.5xB) Microfuge tube of pARA-R (RP) Microfuge tube of restriction enzymes BamHI and HindIII(RE) Microfuge tube of distilled water (dH20) Equipment and Supplies Disposable gloves and lab goggles P-20 micropipette Tip box of disposable pipette tips 2 1.5-mL
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