Biodegradation of Hydrocarbons from Crude Oil by Pseudomonas Putida

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Biodegradation of Hydrocarbons from Crude Oil by Pseudomonas putida

A Project done under the guidance of
Dr. K. Bharathi Department of Biotechnology.
Submitted to the faculty
Department of Biotechnology
National Institute Of Technology, Warangal (A.P)
Submitted By Febin P. Nalpady, Anzal Rahman, Shruti Sharma, Sindhuja Nandiraju, Giraboina Kranthi Kumar NATIONAL INSTITUTE OF TECHNOLOGY
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In quantitative terms, crude oil is one of the most important organic pollutants in marine environment and it has been estimated that worldwide somewhere between 1.7- 8.8×106 tons of petroleum hydrocarbons impact marine waters and estuaries annually. Reports have been appearing since last three decades on the biodegradability of crude oil by bacteria which can use hydrocarbons as source of carbon and energy. A way to mitigate the effects of oil spills is bioremediation. 2.1 Bioremediation It is a process by which chemical substances are degraded by bacteria and other microorganisms. The use of these microorganisms has been successfully applied for the treatment of waste and wastewater in controlled systems. Several research studies have recently been performed to investigate the use of bioremediation for oil-spill cleanup in seawater, freshwater and terrestrial areas. The technique has been found to have a potential for broad applications in terrestrial and freshwater environments for treating soils and sediments contaminated with oil and other substances, as well as for coastal environments impacted by oil spills. Water is a more sensitive medium than soil and requires different remediation techniques. Spills to surface water are easier to clean up than spills to groundwater, for obvious reasons. It is not only much harder to see the extent of the contamination, but also to remove the source of the contamination as, for

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