Introduction The purpose of this lab was to complete a variety of tests that are involved in determining a complete blood count including; a red blood cell count (RBC count), a white blood cell count (WBC count), a white blood cell differential (WBC differential), hematocrit, hemoglobin, and to calculate the mean corpuscular hemoglobin concentration (MCHC). In order to better understand and perform the tasks of this lab we needed to become more knowledgeable about the background components of blood itself. Blood is a liquid connective tissue and its functions include transportation, protection, and regulation. Blood transports oxygen and carbon dioxide between the heart and lungs, as well as waste products to the kidneys and hormones throughout …show more content…
To begin this portion of the total blood cell count a finger prick was completed. In order to do this gloves were put on the participant’s hands and the finger being used to prick was thoroughly cleaned with an alcohol pad (allowed to air dry). Next a sterile lancet was used to stick the cleaned finger until blood appeared. Holding the hand down, two capillary tubes were filled at least two thirds of the way full with the participant’s blood. After the capillary tubes were filled they were sealed by pressing the clean end into the clay pad. Both capillary tubes were then placed onto the centrifuge for three minutes to spin, (which separated the elements of blood). The group then used a hematocrit card reader to measure the percentage of red blood cells. To do this the bottom of the capillary tube was placed on the scale at 0 and the top of the plasma at 100. The value at the top of the red blood cell portion indicates the percentage of whole red blood cells. Materials for collecting the white blood cell differential information included three clean glass slides, a sharp, wrights stain, a buffer for wrights stain, bibulous paper, a stacking rack, and a microscope. This experiment also requires a finger prick, if more blood was needed then it was performed again as previously stated. A small drop of blood was placed on two of the three clean slides. The third clean slide was used to smear the blood on each of the slides containing the drop. The slides were to be held flat while the third slide was used to push the blood down the slide in a thin layer. After a thin blood smear was created on both slides the blood was allowed to air dry for the staining process. Following the slides drying, they were placed on a stacking rack with the blood
Complete Blood Cell Count (CBC): White blood cell count (WBC), Red blood cell count (RBC), Hematocrit (Hct), Hemoglobin (Hgb),Platelets (Plt)
To identify red blood cells, basophils, eosinophils, monocytes, lymphocytes, neutrophils, sickle cell anemia and leukemia.
Exercise 11: Blood Analysis: Activity 1: Hematocrit Determination Lab Report Pre-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. 1. Hematocrit Your answer : a. refers to the percentage of white blood cells (WBCs) in a sample of whole blood. Correct answer: b. of 40 means that 40% of the volume of blood consists of RBCs. 2. A buffy coat layer You correctly answered: d. is all of the above. 3. The diagnosis of anemia indicates You correctly answered: a. a lower-than-normal hematocrit. 4. Polycythemia refers to You correctly answered: c. a significant increase in RBCs.
In this paper I will answer some questions about blood and related issues. Some of the questions I will answer are: what is the significance of a lower than normal haematocrit? what is erythropoiesis?why would the level of leukocytes be higher in an individual who has been infected with a parasitic disease. In regions where malaria is endemic, some people build up immune resistance to the malaria pathogen. Which WBCs are responsible for the immune response against pathogens? How do they function?
In the Core Lab, I was assigned to hematologist named Melissa. She was very experienced in her work since she knew how to operate machines that help her detect any abnormalities in the blood samples. Regularly, the hematologists and other lab workers would work independently, and I like the method of independent labor because there won’t be any obstacles while testing in the lab. I also liked observing the blood samples through microscope and see whether the blood have any kinds of disease or irregularity. The hematology was very interesting and enjoyable; I was able to experience and learn that hematology is another way to test and figure out life threatening unusualness in the body.
The goal of this experiment is to determine the blood types of the samples given and to learn what interactions occurred to each blood type. Determining an individual’s blood type and how it reacts with Anti A, Anti-B, and Anti Rh serums played a crucial part in this experiment. The researcher concluded that agglutination (clumping) occurred in some of the blood samples. For example, Mr. Smith’s blood reacted with Anti-A and Anti-Rh serums (antibodies) allowing the researcher to determine the blood type is A. Mr. Jones’s blood reacted with Anti-B serum but it did not react to Anti-A or Anti Rh allowing the researcher to believe that the blood type is B. Mr. Green’s blood reacted with all serums and caused a reaction to occur resulting the blood type to be AB positive. Mr. Green’s blood also had a positive marker for Rh factor. However, Ms. Brown’s blood had no reaction at all and the researcher determined if no reaction occurred then the sample had no antigens but proved to have some antibodies, resulting in blood type to be O. The purpose of this experiment is to determine whose blood has type A, B, AB, or O.
When foreign antigens enter the body, chemical signals are dispatched to different parts of the immune system and send it into action. B lymphocytes produce the antigen specific antibodies. The antibodies will then attach to the antigen/virus and T lymphocytes will attack the antigen that the antibody has bound itself to. Once the antigen has been eliminated phagocytes, which are cells that absorb bacteria and other small particles/substances, will come through and absorb the remains of the antigen/infection. In a forensic setting antibodies can also be used to identify an unknown blood/protein. The purpose of this experiment is to use the process of immunoprecipitation to identify an unknown host protein.
The Journey of a red blood cell begins inside the bone, where the blood cell is made which is the bone marrow. The red blood cells travel around the body within capillaries. Then the deoxygenated red blood cell makes its way to the heart in the vena cava. After the blood cell has made its way through the heart the right atrium (the cell enters the right atrium first) contracts and pushes the blood cell through the tricuspid and into the right ventricle (the parts where the blood cell enters second which is located in the bottom right corner of the heart.) Next the right ventricle again contracts and pushes the blood cell out of the heart through the semi lunar (the section of the heart where the cell enters third). Then finally the deoxygenated
In 1984, the Coen brothers made their debut with Blood Simple, a neo-noir film that would not be their last. While the Coens are influenced by film noirs, they, like filmmakers before them, draw inspiration from the works of Dashiell Hammett and James M. Cain as well. Even though the Coens utilize concepts and conventions from film noir and hard-boiled fiction, they do not shy away from reinventing these formulas in Blood Simple. Before exploring how the Coens reinvent the formulas from past works, it is important to understand what preceded and influenced neo-noir: film noir.
The following Red Cell Analysis will seek to provide the best suitable data collection programs to be utilized in gathering intelligence on the Islamic State of Iraq and Syria (ISIS), an explanation on which members of the Intelligence Community (IC) would be the best collectors of intelligence on ISIS and lastly, what intelligence analysis strategies would be the most effective on ISIS. Additionally, the origins, funding, and capabilities of ISIS will be provided for reference.
Intraoperative ultrasonography has been used to determine whether there is metastatic disease to other organs. Laboratory test will exhibit elevated WBC and AST. A complete blood count (CBC) is a common laboratory test used to measure blood counts (or the number of blood cells circulating in the bloodstream). A CBC measures the level of red blood cells (which carry oxygen throughout the body), white blood cells (which fight infection), and platelets (which help with blood clotting) in a blood sample. A CBC also measures hemoglobin (a protein in red blood cells that carries oxygen) and hematocrit (the ratio of red blood cells to
Can be performed on either a blood smear sample or a hemocytometer using a standard microscope system. for a hemocytometer, the gridded area is scanned for the counting purpose, to identify the numbers of different white cells, a blood film is made, and a large number of white blood cells (at least 100) are counted. This gives the percentage of cells that are of each type. By multiplying the percentage with the total number of white blood cells, the absolute number of each type of white cell can be obtained. Although the manual method is more laborious and time consuming, it is useful in cases where automated analyzers cannot reliably count abnormal cells,
Methodology and Principal Findings: First of all, we made every materials that we need ready. First step was wiped a finger with alcohol, stacked it with lancet. Then, we collected amount of blood in a capillary tube. We left it about one and half hour to collect a plasma with white blood cells. Second step prepared in bottom Petri dish with tap water and filter paper, placed the bent rod
When God created the world he knew the structure of the cell, he had every detail planned out. This shows how amazing and detail oriented our God truly is. In this lab we explored his creation more and looked at individual cells using microscopes. The microscopes were introduced in the first lab and were important to look at the cells more closely. While using either premade samples or the student’s cheek cells, the students learned how to use the microscopes well through adjusting the slide to find cells or focus the microscope so one could see the slides clearly. Through the microscope, the nucleus is visible because it is dyed darker than the other parts of the cell. This was true for all of the light microscope slides. The students were introduced to the inverted microscope containing mouse cells. The students
Figure Caption: The graph above represents the mean time, in seconds, for hemolysis to occur in Bovine blood cells when placed with three different solutions with varying sizes: Ethylene glycol, Diethylene Glycol, and Triethylene Glycol. To start off, Hemolysis is the process in which red blood cells rupture and components like hemoglobin are released in the solution. In this experiment, the time in which hemolysis occurred was measured within three different glycol solutions: Ethylene glycol, diethylene glycol, triethylene glycol ranging in different sizes. As soon as the blood was added to the glycol solutions the process of hemolysis occurred.