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Blood Cell Lab

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Introduction The purpose of this lab was to complete a variety of tests that are involved in determining a complete blood count including; a red blood cell count (RBC count), a white blood cell count (WBC count), a white blood cell differential (WBC differential), hematocrit, hemoglobin, and to calculate the mean corpuscular hemoglobin concentration (MCHC). In order to better understand and perform the tasks of this lab we needed to become more knowledgeable about the background components of blood itself. Blood is a liquid connective tissue and its functions include transportation, protection, and regulation. Blood transports oxygen and carbon dioxide between the heart and lungs, as well as waste products to the kidneys and hormones throughout …show more content…

To begin this portion of the total blood cell count a finger prick was completed. In order to do this gloves were put on the participant’s hands and the finger being used to prick was thoroughly cleaned with an alcohol pad (allowed to air dry). Next a sterile lancet was used to stick the cleaned finger until blood appeared. Holding the hand down, two capillary tubes were filled at least two thirds of the way full with the participant’s blood. After the capillary tubes were filled they were sealed by pressing the clean end into the clay pad. Both capillary tubes were then placed onto the centrifuge for three minutes to spin, (which separated the elements of blood). The group then used a hematocrit card reader to measure the percentage of red blood cells. To do this the bottom of the capillary tube was placed on the scale at 0 and the top of the plasma at 100. The value at the top of the red blood cell portion indicates the percentage of whole red blood cells. Materials for collecting the white blood cell differential information included three clean glass slides, a sharp, wrights stain, a buffer for wrights stain, bibulous paper, a stacking rack, and a microscope. This experiment also requires a finger prick, if more blood was needed then it was performed again as previously stated. A small drop of blood was placed on two of the three clean slides. The third clean slide was used to smear the blood on each of the slides containing the drop. The slides were to be held flat while the third slide was used to push the blood down the slide in a thin layer. After a thin blood smear was created on both slides the blood was allowed to air dry for the staining process. Following the slides drying, they were placed on a stacking rack with the blood

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