Comparing Coproantigen Elisa And Serology Indirect Elisa On Liver Flukes Infection
1720 WordsApr 18, 20167 Pages
AGR3AH- ANIMAL HEALTH
Comparison of Coproantigen ELISA and Serology indirect ELISA on liver flukes infection.
Hiu-Fung Yannis Wong
In this experiment, both Coproantigen ELISA and Serology indirect ELISA were carried out on samples from a farmer which suspected his herds are suffering from Fasciola hepatica infection. The aim of this experiment is to find out if the samples are positive for Fasciola hepatica infection and discuss the difference between two tests.
Fasciola hepatica, commonly known as the liver flukes, is parasites that are mainly located in hosts’ liver, feeding on blood of its host. The lifecycle of liver flukes start by host consuming plant which the surface were covered by liver flukes’ cysts. The metacercariae would be released from the cysts after they get into duodenum of the host. The metacercariae would then burrow through the intestines and liver, eventually got into the bile ducts (Kaewkes 2003). They spend approximately 8 weeks to become adults, which can produce up to 25,000 eggs per day. Eggs would be exterminated via faeces, which start the cycle again (Swales 1935). Fasciolosis is a serious global problem as a disease. In 1999, there were approximately up to 40 million sheeps and 6millions cattle fed on pastures which liver fluke is endemic. Fasciolosis does not only kill cattle but also reduce milk productivity and quality. Graziers need to spend about $10 million per year just to prevent fluke itself and production