Background
The gene that we are interested in for this project and for the duration of this semester is DMAP1. Not a lot is known about this is a gene, however in other organisms, it is involved in the methylation of DNA. DMAP1 is responsible for encoding a protein that associates with another protein that methylates DNA. The methylation of DNA alters the DNA structure and makes it unavailable to transcription factors, so the mutation of DMAP1 could lead to changes in transcription and gene regulation. For example, if DMAP1 were mutated, less DNA methylation might occur and more areas of DNA could be accessible to transcription factors and ‘active’, making more genes transcribed than should be. Gene alterations are important as they can lead
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This wings clipped strain will provide the transposase enzyme, allowing the P-element to be mobilized, and provides a dominant marker (stubble), allowing us to recognize flies in which the P-element could have been mobilized. We are hoping that, because the P-element is so close to the DMAP1 gene, that mobilization will cause the P-element to insert into the DMAP1 gene. Although P-elements can insert anywhere onto the genome, they tend to insert into the beginning of genes, and because DMAP1 is so close to where the P-element has inserted, it is likely that it will insert into DMAP1 when mobilized. The P-element could insert anywhere on chromosome 2, as well as anywhere else in the genome. After mobilization, it is possible that chromosome 2 will no longer have the insert, or could have multiple inserts at different locations. If the P-element does insert into the DMAP1 gene, it would deactivate it, as its DNA “code” would no longer be correct, and it would not produce a functional protein if transcribed. As there is no guarantee that the P-element will insert into DMAP1, we will do multiple crosses. The mobilization cross will involve flies of genotype Xyw/Y;P*/P*;+/+ crossed to Xw/Xw;Sp1/CyO;Sb Δ2.3/TM6B. We will then collect progeny of genotype Xw/Y ; P*/CyO; Sb Δ2.3/+, which will be recognizable by their curly wings and stubble. The presence of the stubble phenotype tells us that the wings-clipped transposase element was present to allow the P-element to mobilize. When the P-element is mobilized, it may move in each individual cell that will form sperm at the end of meiosis. For this reason we will collect males individually in preparation for the next cross, as each male could have the P-element inserted into a different position. We will then take each male
The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.
The goal of this study was to induce a deletion in the DMAP1 gene on chromosome two in Drosophila melanogaster through P-element mobilization. The DMAP1 gene may be an essential gene, however not much is known about it. We attempted to uncover the function of DMAP1 by creating a series of genetic crosses and selecting for brown-eyed non-stubble male flies that may have the deletion. To test whether these flies had the deletion, we produced PCR products and ran them on an agarose gel, which resulted as inconclusive. We created a balanced stock of flies homozygous for the deletion to see if the
The mapping of human genes has allowed for certain genetic disorders to be identified according to the genes that it is affecting. This has created a map that other individuals' genes can be compared to in order to determine any mistakes or any alterations that may lead to the development of a disease. Any changes in epigenetic
Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.
Drosophila Melanogaster, commonly known as fruit flies, are highly important model organisms in pertaining to biological research. The logic behind their recurrent use is due to their: easy culture in the laboratory, brief generation time, and ability to produce large numbers of offspring. In this report, we created isolated virgin D. Melanogaster from the original three populations we were given and then created crosses between them. Upon observation, we noticed an unusual mutant that arose from two of the three created crosses. We suspected that this genetic mutation had previously been discovered and named.
Now you have determined some facts about the grounded allele and the trait that it causes. Given what you know, do you expect the mutant F1 flies to be homozygous or heterozygous for the allele that causes the grounded trait? According to your reasoning, if you mated two mutant F1 flies, what percentage of flies would you expect to be wild type versus mutant in the F2 progeny? Draw a Punnett square of this cross to justify your answer.
There were eight different phenotypes among the progeny. The highest phenotypic frequency was the w+m+f+ at 40% of the progeny. The lowest was the w+mf+ with only 2 % of the progeny (Table 3). The sum of the recombinant frequencies between genes, table 4, was used to determine the gene distance. The recombinant frequency was determined by counting the number of individuals whose genes differed from that of the parental type. For example, how many individuals white eye gene, and miniature wing gene, differed from both wild-type or both mutants. Recombination occurred between the white and miniature gene 33 times. Recombination occurred between the miniature and the forked genes 31 times. Recombination occurred between the white and forked genes 44 time. Double recombination occurred 10 times. Therefore, genes w and f are 64 m.u. apart, m and w are 33 m.u. apart, and m and f are 31 m.u. apart (Figure
11. The progeny of a Drosophila female (heterozygous at three loci: y, ct, and w) crossed to a wild type male are listed below:
we said goodbye and placed them in the fly morgue. We allowed the F2 larval
Methylation is the process where methyl groups are attached to the sequence of DNA code letter and to the histone groups that the DNA encircle; whereas, acetylation is the process where acetyl groups are attached to the histone tails. The methylation decreases gene expression while the acetylation increases gene expression. Epigenetic inheritance mentions in the article supports the content that we cover in class, in which epigenetic inheritance means modification to DNA other than sequence that can be altered by environment or diet and can be passed on to next
Methyl groups turn genes on or off by affecting DNA-protein interaction in a genome. Genes attached by methyl groups are unable to interact with the protein, halting transcription of the gene. It was believed that epigenetic changes (like the methylation
This experiment looks at the relationship between genes, generations of a population and if genes are carried from one generation to another. By studying Drosophila melanogaster, starting with a parent group we crossed a variety of flies and observe the characteristics of the F1 generation. We then concluded that sex-linked genes and autosomal genes could indeed be traced through from the parent generation to the F1 generation.
DNA is associated with specific proteins called histones which compact and package the chromosomes into the nucleus. The resulting DNA-protein complex is called chromatin. The packaging of DNA into chromatin can impact the ability of the numerous proteins and enzymes to gain access to specific genes before activating their transcription. The chromatin structure can be altered, either being loosened to facilitate gene expression or tightened to limit gene expression. These alternations are largely mediated by reversible modifications of the histones through a dynamic, tightly regulated process called chromatin remodeling. Chromatin remodeling mediates differential gene expression and is therefore crucial for development, cell type-specific gene
Epigenetics can be hereditable or environmental factors that affect the expression of genes and lead to changes in gene expression. Unlike genetics, epigenetics does not only have to do with which genes are passed down to the offspring and the DNA sequence. The environmental conditions of the offspring’s parents impact the genes in their eggs and sperms by “switching on” certain genes and “switching of” others (Dowshen). Since the genes expression of the gametes are affect, the phenotypes of the offspring will change. Even in a person’s lifetime, environmental factors such as stress, chemical exposure, and diet can continue to impact gene expression through DNA methylation. During DNA methylation, a methyl group is randomly added to a 5-carbon cytosine ring, making 5-methylcytosine and these groups inhibit transcription. (Cheriyedath). Due the fact that transcription is not possible, the expressing of the genes in that section of the DNA strand will be suppressed. The attachment of the methyl group to DNA is not determined, which means that
Modification of damaged DNA seems to be an understudied subject, there is much to understand on the restoration of DNA damage, repair and DNA methylation. Genomic DNA can be modified by methylation but much of it is affected on a gene when silenced. When epigenetic modification has been implicated with cancer and aging it causes DNA methylation to also have an impact on the double strand of DNA analysis. Modification as such provoke deteriorating changes like aging found in multicellular organisms and DNA damage may magnify biochemical pathways that regulate a cells growth or control DNA replication with DNA repair. In the article “DNA Damage, Homology-Directed Repair, and DNA Methylation” written by Concetta Cuozzo, Antonio Porcellini, Tiziana Angrisano, et al. they hypothesize how DNA damage and gene silencing may induce a DNA double-strand break within a genome as well as when DNA methylation is induced by homologous recombination that it may somewhat mark its reparation through a DNA segment and protect its cells against any unregulated gene expression that may be followed by DNA damage. The experiments used to demonstration how gene conversion can modify methylation pattern of repaired DNA and when that occurs methylation is able to silence the recombined gene. When exploring the molecular mechanisms that link DNA damage and the silencing gene then there is an induced double strand break that can be found at a specific location or DNA sequence in where the