Drosophila Melanogaster

In this experiment we tested to see what the offspring of an unknown cross of an F1 generation would produce. After observing the F2 generation and recording the data we found some of the Drosophila showed mutations, two in particular. The mutations were the apterus wings, and sepia eyes. After collecting our data through observation, a Chi-test was conducted resulting in a Chi-value of 5.1 and a p-value of .2. Since the p-value was greater than 0.05, there was no significant change in the data. This proved that the Drosophila flies still followed the Mendelian genetics of a 9:3:3:1 ratio.

The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.

Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.

melanogaster, leaving B and D to be our mutants. Before crossing our populations, we made not of each one’s phenotype in order to see how crossing them would affect their phenotypes: Population B flies had no wings and red eyes, population D had full wings and black eyes and population G had full wings and red eyes. We expected the resulting phenotypes to be some sort of combination, revealing which traits were dominant. However, what we did not expect was the abnormal mutant that arose in a couple of our populations.

The motivation of this lab report is to use Mendel’s Laws of Inheritance to analyze and predict the genotypes and phenotypes of an offspring generation (F2) after knowing the genotypes and phenotypes of the parent generation (F1). The hypothesis for this experiment is that the mode of inheritance for the shaven bristle allele in flies is autosomal recessive in both male and female flies.

It would be expected that the mutant F1 flies would be heterozygous for the allele responsible for the grounded trait. If two F1 flies were mated, the percentage of flies that would be expected to be wildtype in the F2 generation would be 25% mutants given that the mutant allele (ap) is predicted to be recessive and, leaving 75% to be wildtype (ap+).

Table 1 shows the phenotypes of the F1 flies produced by crossing P1 wild-type females and P1 no-winged mutant males. The results of that cross was that there were forty seven wild-type females and fifty three wild-type males. Therefore there was a total of one hundred wild-type flies that were produced. The observed phenotypic ratio of wild-type flies and no-winged mutant flies was 1:0 (wild-type: no-winged). The predicted phenotypic ratio if the no-winged mutation is autosomal recessive would be 1:0 (winged: no-winged).

A. If the wingless mutation is autosomal Dominant on either the female or male then we can expect all of the

Introduction For centuries, researchers have used Drosophila melanogaster, the common fruit fly, to study genetics. The benefits of using the fruit fly includes: its relatively short generation time, its large amount of available offspring for data, it is easy to store and handle in the laboratory and it is easily and cheaply obtained. Cross-breeding of four types of fruit flies were used in this experiment including: wild type males with normal wings vs. vestigial wing females, wild type males with red eyes vs. white eyed females, wild type male with red eyes vs. sepia eyed females, and wild type males vs. wild type females. In basic mendelian genetics, the terms dominant, recessive and sex-linked are used to describe the different types

This was hypothesized because they are different from the wild type traits. We also hypothesized that the white eyed trait was an X-linked trait. After the wild type males were crossed with the sepia females, no sepia progeny were present in the F1 generation, suggesting that the sepia trait is autosomal recessive. The F2 generation of flies further suggests that the sepia and vestigial traits are autosomal recessive. The progeny of the cross between vestigial females and the wild type males showed a 3:1 ratio of wild type flies to vestigial flies.

The Drosophila melanogaster is one of genetics most studied organisms. This is due to the Drosophila melanogaster being an excellent model organism. The Drosophila melanogaster has a short lifespan and is genetically similar to humans (Adams 2000). This experiment had three major goals. The first goal of this experiment was to determine which eye colors, body colors and wing type are dominant or recessive. The second goal was to determine if the gene for eye colors, body colors and wing type are on an autosomal or a sex chromosome. The third goal was to determine if eye colors, body colors and wing type are physically linked or independently assorting (Morris and Cahoon). First

For our first generation (F1) of flies we chose to cross apterous (+) females and white-eye (w) males. We predicted that the mutation would be sex linked recessive. So if the female was the sex with the mutation then all females would be wild type heterozygous. Heterozygous is a term used when the two genes for a trait are opposite. The males would all be white eye since they only have one X chromosome. If the males were the sex that had the mutation then all the flies would be wild type but the females would be heterozygous.

Drosophila Melanogaster commonly known as a fruit fly, is a model organism, used by scientist / genetics all around the world to study, the genetic component, its genetic mutations, it’s genetic relations between different mutations through learning and understand the principle behind gene transformation, from one generation of flies to the other. The underlaying reason why scientist favor it among other eukaryotes its due its small size, and it’s high rate of reproduction within days. Drosophila Melanogaster was first used as a model organism by Thomas Morgan “ in 1911…who investigated the inheritance pattern of different trait that had been shown to follow the X-linked

The Drosophila melanogaster organism has been favored throughout decades as a model organism for its ability to be cultured in mass, has a short generation time, and, with a myriad of mutations to be mapped to the organism, organisms with certain mutations can be ready at hand to further study. The inception of the D. melanogaster research started in Columbia University at the hand of Thomas Hunt Morgan. Morgan’s different views in genetics led to questioning Mendel’s past research and finding faults in his inheritance patterns and ratios. Mutations in fruit flies were discovered in a small fraction of flies in 1907 by Frank Lutz who found some flies had extra venation and successfully bred them to all have extra venation over eight generations.

This experiment looks at the relationship between genes, generations of a population and if genes are carried from one generation to another. By studying Drosophila melanogaster, starting with a parent group we crossed a variety of flies and observe the characteristics of the F1 generation. We then concluded that sex-linked genes and autosomal genes could indeed be traced through from the parent generation to the F1 generation.