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Essay on E Coli and Salmonella in Swine Finishing Barns

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A total of 50 commercial swine finishing barns were recruited and sampled from 5 states during June – October 2011. A total of 30 fresh fecal samples were collected from the floor of each barn, 5 samples from 6 different pens, when the animals were at the end of the finishing phase and within 30 days of marketing. At the time of collection, barn managers were given a survey to capture data relating to antimicrobial use practices for the sampled group of animals beginning in the farrowing phase.
Fecal samples were shipped overnight to The Ohio State University for bacterial culture of E. coli and Salmonella, and selective enrichment with media containing cephalosporins:
E.coli: For blaCMY-2 selective E. coli culture, a 4 gram aliquot of …show more content…

Pulsed Field Gel Electrophoresis (PFGE) was utilized for comparison of isolates for clonality. Answers from the antimicrobial use survey were compiled and analyzed for association with recovery of blaCMY-2 and blaCTX-M genes using Chi-square and Fisher’s exact tests.
Results
A total of 1,495 fresh fecal samples were collected from 50 commercial swine finishing in 5 states between the months of June and October 2011. The barns contained an average of 1,236 pigs with an average pen density of 35 pigs/pen and had an average of 96 days on feed in the finishing barn.
The antimicrobial use survey (Table 1) indicated that ceftiofur treatment in the early phases of production was common with 58% of farrowing barns and 72% of nursery barns administering the antimicrobial. In the finishing phase, 48% of the barns administered ceftiofur therapy to sick pigs with an average within-barn treatment proportion of 2.2%. Administration of other antimicrobials during any or all production phases was common among the survey responses with 88% of barns reporting delivery through feed, 12% through water, and 74% of barns reporting treatment of individual swine with parenteral antimicrobials.
Commensal enteric bacteria containing the blaCTX-M cephalosporin resistance gene were detected in a total of 8 barns (16%); 10 E. coli isolates from 5 barns in Ohio, 12 E. coli isolates from 1 barn in Michigan, and 2 Klebsiella

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