Effect of Spices and Herbs on Inhibiting Bacteria Growth

Pour out 10mL of vinegar into the film canister, it should fill up about one fourth of the canister.

AIM – The aim of the experiment is to determine the relative effectiveness of several anti-microbial substances on developing pathogens. (E. coli)

10.Clean up: Rinse reaction vessel, empty collection box, dry table as needed, cleanup up any baking soda spills..

Due to the microquantities being used, all chemicals will be disposed up with running water. Gloves, an apron, and splash goggles will be used to protect the eyes and skin.

Store dangerous, unpredictable chemicals in a flame resistant bureau or if non-combustible, or if in little sums, in a fume cupboard.

After the required disinfection time has passed, remove all tools and implements from the disinfection solution with tongs or gloved hands. Rinse the tools and implements well in warm running water, and then pat them dry. Store the dry, disinfected tools and implements in a clean, covered container until they are needed.

According to the Centres for Disease Control and Prevention (CDC) a minimum of 2 million people become infected with antibiotic resistant bacteria and an estimated minimum of 23, 000 people die each year as a direct result of those infections . As an increasing number of infectious diseases are becoming resistant to numerous allopathic medicines , for example meticillin-resistant staphylococcus aureusis or MRSA, more research is being conducted into alternative treatments derived from naturally occurring substances. Having heard about the

7. Clean test tubes with test tube cleaner and warm soapy water in the sink.

The methodology involved using the plant’s flower bud by washing it in sterile water, dried, and crushed into a powder. Eventually it was converted into an ethanol and methanol extract and observed for any effects on MRSA strains (Yadav et al., 2011). The results showed that both extracts had inhibitory effects on various MRSA strains and could have the potential for use as an antibacterial agent for multidrug resistant strains (Yadav et al., 2011). In some ways the study suggests that cloves can play a role in treating bacterial infections, but it is possible that converting it into an ethanol or methanol extract might have improved its effectiveness on MRSA strains. Using clove capsules to cleanse the body of microorganisms may or may not be as effective since the concentration of cloves per dose is different from the effectiveness of an ethanol or methanol extract used as an antibacterial

Allicin helps protect garlic and only becomes active when garlic is crushed or smashed. Allicin works as garlic immunes system acting to protecting the garlic from reacting with anything unwanted. Allicin works as both a competitive and noncompetitive inhibitor to protect garlic from outside influences. As seen in Fig 5 as a competitive inhibitor Allicin will beat the substrate to the active site and therefore stop the reactants to produce anything. Allicin also works a noncompetitive inhibitor, as seen in Fig 6 the Allicin inserts itself in the Allosteric site and therefore distorts the active site so the substrate cannot react with the

Do not put your face close to the petri dish and do not open it ever again once it has been sealed up.

Figure 1.1 reveals that there was no supportable correlation between the combined use of ethanol and capsaicin against the gram-negative bacteria, Serratia. The results varied in an unpredictable manner. According to the Figure 1.1, the 5 mL solution of pure ethanol, on average, resulted in higher zones of inhibition than the other treatments, which was expected due to ethanol’s antimicrobial properties. But, the ethanol and seed solution, which warranted the best possible results, produced straight zeros across the board. In the 5 mL solution of ethanol and water, we saw effects from both seeds and seedless solutions.

Garlic is one of the most well known and widely used spices in the world, and for good reason. With its strong taste and bright flavor, just a clove can spice up any meal. It's also one of the most potent natural antihistamines, and is incredibly high in natural antioxidants.

Do disinfectants - sodium hypochlorite (Bleach), Lysol©, and Allium sativum (Garlic) extract - reduce the development of evolutionary bacterial resistance in Escherichia coli (E. coli) and which disinfectant has the greatest effect on the bacteria?

D. Before using the glass electrodes, they must be washed in warm soapy water and put in the UV steriliser to ensure they are clean and free from bacteria. After the treatment they should be washed and wiped over with disinfectant and sterilised before being used again.