Elodea Temperature Experiment

27. Heat the water to 40°C with an alcohol lamp by setting up the apparatus as shown in figure 3 below:

mL cylinder to the beaker on the stir plate and empty it into the beaker. Place the pH probe in the beaker and record the pH in the data table. Drag the beaker to the red disposal bucket. Double-click the bottle of NaHCO3 to move it to the Stockroom counter. Repeat steps 5 and 6 for KNO3.

The hypothesis tested in this experiment was, if the temperature of enzyme catalysis were increased, then the reaction rate would increase, because enzyme-catalysis reacts by randomly colliding with substrate molecules, and the increase in temperature increases the speed of collision or reaction rate. The final data collected for the experiment was positive with my hypothesis. The coffee filter, covered in potato solution, sank and rose at a faster pace in the hydrogen peroxide when the temperatures were raised.

9) Trial E: Remove the syringe and empty the beaker. Add a Thermometer to the beaker. Add 200 mL of Room Temperature water to the beaker and heat with a Bunsen Burner until it reaches 100° C. Remove the Bunsen Burner. Repeat Steps 5 & 6.

Pour approximately 50 mL of room-temperature distilled water into the glass beaker by using the estimated volume on the beaker.

In this lab, you will be experimenting with how different variables affect the rate of photosynthesis.

XII. Take the 250 ml beaker to your lab bench. Set up a gravity filtration with a plastic funnel, folded wet filter paper, and an Erlenmeyer flask. Pour the content in the 250 ml beaker slowly through the filter paper. Wash the filter paper with deionized water. Dispose of the filtrate in the proper labeled waste container.

After the whole set up is done we will switch on the lamps, measure the initial length of water and start the stopwatch after as we see 2 or 3 bubbles (which signify process has started).

Rinse out beaker and dry it, preparing for next trial. Also reset stopwatch. Repeat steps 10-16 two more times until you have completed three trials in total. Measure the temperature of the hot water in celsius and record it to ensure consistency in temperature throughout the experiment.

Next get the three types of waters with the different temperature and measure 50ml of water. Take the thermometer and write down the temperature of the water . Then measure 4 grams of sugar of the balance with the wax paper. Then you are going to take the sugar and pour it in the hot water and immediately start the stopwatch and start timing the sugar dissolving. Stir with a straw until fully dissolved.

Then, put the test tubes in the matching numbered flasks. III. Put the aquarium tubing onto the 1-mL pipette and once you have done so add the 1-mL pipette to each test tube IV. Put the flask with the test tube and the pipettes in the water bath at the temperature of 30 degrees Celsius. Make sure you record your time and wait 5 minutes with the tubing unclamped.

Water can be kept a constant by keeping the same amount of water in the beaker. Temperature- Enzymes are used in photosynthesis and the respiration of the plant. Therefore, increasing the temperature will increase enzyme reaction and the photosynthetic rate until a certain point is reached when the enzymes denature. The temperature can be kept somewhat a constant by performing the experiment in one session, when the air temperature shouldn't change enough to affect water temperature. A transparent glass block will also be placed in front of the lamp to retain some of the heat from the lamp.

8.After 5 minutes, remove test tubes from the water bath set in test tube holder and record results. Also turn off hot plate.

There are three main steps in preparing the experiment, which are a) experiment principle & design; b) calibration of resistance temperature detectors (RTDs) and thermocouples (TCs) and c) Set up of equipment.

What is the effect of altering the distance (±0.05 cm) of the Elodea Canadensis aquatic plant from a light source at (5cm, 15cm, 25cm, 35cm and 45cm) on the photosynthetic rate by counting the number of oxygen bubbles rising up the test tube per minute (min^(-1))?