Epithelial Food Lab

This data was analyzed by calculating the cumulative change in weight for each dialysis bag. This was done from subtracting the weight of each bag from the initial weight of the bag. Doing so, allows the weight of each bag to be initially zero. For that, we must calculate the corrected cumulative change in weight. For each time interval of 10 minutes, we subtracted the change in weigh of bag #1 (tap water) from the weight of each bag at the specific time measure- this corrected any oscillations.

In conclusion, the results of the experiment not only demonstrates diffusion but also displays how permeable membranes, such as a dialysis tube, can be. The results support my hypothesis. The glucose level changed in the experiment from 250 to 100 proving that the tube allowed glucose in, but was impermeable to starch. The experiment reflected the results of biological simulation: glucose molecules crossing membranes into the intestinal

Filled 4 beakers with de-ionized water up to 150 ml. Labelled each beaker with labeling tape to differentiate the sugar concentrations (0 M, 0.25 M, 0.5 M, and 0.75 M). 2. Prepared 4 separate dialysis tubing bags. a. Got the tubing from the container of distilled water.

•Prepare to start stopwatch put marble chips in with acid & put bung on conical flask whilst starting stopwatch every minute until 8 minutes starting from 0s take a measurement of how much air there is in measuring cylinder (cm3) When finished this with the first concentration of acid clean out the conical flask and refill the measuring cylinder & repeat experiment.

AIM The aim of the experiment was to observe the effects the concentration as on the rate of osmosis. HYPOTHESIS The smaller the amount of concentration the quicker osmosis will occur.

My prediction is water will move into the dialysis bag due to osmosis and waters ability to dissolve other substances, a molecule of water is small. While the sucrose molecules are large and will not be able to pass through the dialysis bag tubing

The dialysis tubing experiment will test the effect that different concentrations of sucrose within the semi-permeable membrane will have on the net direction of water diffusion. The solutions within the

When the different dialysis tubes with different substances were placed in water, diffusion took place; this is when the particles moved from a high concentration region to a low concentration region. Osmosis is the act of moving through a semipermeable membrane. Therefore the lab required a semipermeable membrane; however a semipermeable membrane is only found in cells so students used dialysis tubes. The dialysis tubes used, allowed the substance within to flow outside of the tube, essentially working as a semipermeable membrane. The osmolarity for the substances were different, the osmolarity can be determined by the amount of solute particles in a liter or per liter. In the lab there was one isotonic solution this was the blue solution. The mixture was isotonic because both the solution outside of the tube and inside of the tube had the same amount of concentration. During this lab both hypotonic and hypertonic solutions were observed. Hypotonic is when the osmotic pressure is lower than the fluid. Hypertonic is when the osmotic pressure is higher than the fluid. The students were able to observe this with the different color

First, the dialysis tubes were soaked in distilled water. Each of the dialysis tubes were then filled with20 millimeters of a solution of 30% sucrose and tied with its own colored rubber band then weighed to the nearest one-tenth of a gram. Three larger beakers were then filled with distilled water at hot, cold, and room temperature water. Each different labeled dialysis tube was put in its own beaker, blue put into room temperature water (21 degrees Celsius), red put into the hot water (37 degrees Celsius) and yellow put into the cold water (6 degrees Celsius). After fifteen minutes, each dialysis tube was weighed again and the difference was recorded. This process happened every 15 minutes for a total of 60 minutes. Then the rate of osmosis was calculated by dividing the weight difference at 60 minutes by 60 minutes. A bar graph (fig. 4) was then made with the x-axis labeled as temperature, and y-xis labeled as the rate of osmosis

In this experiment, two beakers were used—the left beaker held the varying solutes and the right beaker held the deionized water. Placed in the middle of the two beakers was the dialysis membrane with various molecular weight cutoffs (MWCO), 20, 50, 100, and 200 MWCO. Four different solutes, sodium chloride, urea, glucose, and albumin, were tested in different trials during this experiment based on their molecular weight. Sodium chloride has a molecular weight of 58.44 g/mol, the smallest out of the four, so it was tested in the first trial with 20 MWCO. The left beaker was filled with 9mM of sodium chloride and the right beaker was filled with water. The barrier between the two beakers was dropped, allowing the solutions to have full access to the dialysis membrane. At the end of 60 minutes, the amount of solute that passed through the membrane will be able to be observed on the concentration display. After the amount is recorded, the beakers were flushed and the next solute was added. The second trial with the 20

· Measure out 10ml of acid and add it to the conical flask and start

8. Set up the container for the hot-water bath. Attach two ring clamps, one above the other, to the second ring stand beneath the test tube assembly. Place a wire gauze with ceramic center on the lower ring. Set a third 600 mL beaker, which should be empty, on the gauze and raise the beaker toward the test-tube assembly until it surrounds nearly one-half of the tube’s length. The beaker will pass through the ring clamp without gauze, and the test tube should not touch the bottom or sides of the beaker. The top clamp keeps the beaker from tipping when the beaker is filled with the hot water.

2. Place a piece of filter paper in the funnel so that solid material does not flow through.

Measure the weight of the of the flask with the chips on the balance scale and record it down.

Obtain a piece of filter paper. Measure and record its mass, and then place the filter paper on the funnel. Start the vacuum filtration.