The expectation-maximization (EM) algorithm uses incomplete data to estimate the parameters of probabilistic models, and it has been widely used in machine learning. In this paper, EM techniques are applied to Time-domain fluorescence lifetime imaging microscopy (FLIM) systems for estimating fluorescence lifetimes without measuring the instrument response functions (IRF). The results of Monte Carlo simulations indicate that the proposed approach can obtain comparable or better accuracy and precision performances than the previously reported method.
Introduction: Time-correlated single-photon counting (TCSPC) has excellent timing performances, and it is routinely used for fluorescence lifetime imaging microscopy (FLIM) system [1, 2]. FLIM
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This method is widely used for parameter estimation with incomplete or missing data [10, 11, 12]. In this paper, a new EM-based Lifetime Estimation (EMLE) algorithm is proposed to simultaneously estimate the IRF and lifetime, and it shows better photon efficiency compared with EKF in [9].
Theory: According to the EM theory [10, 11, 12], we assume that {xi, i = 0, 1, …, N-1} are observation values of a random variable x whose density function shown in Fig.1(a) is , (1) where f(x|ξi) is the density function of ith component with a parameter ξi , λj is the component weight satisfying Σλj= 1, j = 0,1, …, L-1, and ϕ = [ξ0, λ0, ξ1, λ1,, ξL-1, λL-1]. ξi and λj can be estimated using {x0, x0, …, xN-1}. An expectation step (E-step) and a maximization step (M-step) are performed iteratively [10, 11], shown in Fig. 1(b).
In E-step, the posterior probability pij is . (2)
In M-step, ϕ that maximizes the expected log-likelihood in E-step can be calculated from , (3) where ϕi = [ξi, λi] is the parameter for the ith component.
a b
Fig. 1 Overview of EMLE a Mixture of the density function b EMLE processing flow.
In a TCSPC-FLIM experiment, we assume that the fluorescence density function is g(t), the IRF is IRF(t), and the measured fluorescence decay is y(t). y(t) is the sum of an additive Poisson noise, v(t) and the convolution of g(t) and
Due to its resolution beyond the diffraction-limit, its axial sectioning capability (the removal of out-of-focus blur), and its high speed imaging, SIM has been applied more than other mentioned super resolution techniques in fluorescence microscopy. In this method that first time was introduced by Neil et
The model parameters are estimated from the EP and therefore the AR can be calculated within the TP (Strong, 1992). Explicitly, the AR which
The data sets for problems 5 and 6 can be found through the Pearson Materials in the Student Textbook Resource Access link, listed under Academic Resources. The data is listed in the data file named Lesson 20 Exercise File 1. Answer Exercises 5 and 6 based on the following research problem:
C. An unknown, rectangular substance measures 3.6 cm high, 4.21 cm long, and 1.17 cm wide.
BSP, S. (2010). How is EM different from light microscopy? Retrieved April 25, 2015, from http://bsp.med.harvard.edu/node/222
based on a Dirichlet prior over the each parameters assuming equal priors on each parameter. And especially using Laplace Smoothing then we can get:
The expected value approach is used to determine the expected value with perfect information (EVPI). The EVPI is obtained by subtracting the expected value without perfect information (EMV) from the EVPI.
The trace statistics ʎ trace and the maximum Eigen statistics ʎ max were used and the results are presented in table 3 and 4 below.
The remaining results used to obtain the graph in the next section can be obtained by, iteratively substituting the parameters shown in table 3 below for the various architectures and various population sizes. The system parameters given in [16] is shown in table 3.
The base of the light triangle, b, was measured first. Then, the light positions, a, were measured f=or the first order (m=1) and second order (m=2) light beams to determine the angle Ɵ for each. This data was organized in an Excel table and used to find the wavelength, λ, of the laser through Equation 1. This value was then compared to the theoretical value for wavelength of the laser, which was 6.328x〖10〗^(-7)m, and the percent error was calculated between the two.
Using a statistical model they created (See Appendix), Entine and Small ended up with the following results:
Following that, the expected values for decision nodes 6 and 7 should also be calculated. The following results were obtained:
Estimating the mixing density of a mixture distribution remains an interesting problem in the statistics literature. Stochastic approximation (SA) provides a fast recursive way for numerically maximizing a function under measurement error. Using suitably chosen weight/step-size the stochastic approximation algorithm converges to the true solution, which can be adapted to estimate the components of the mixing distribution from a mixture, in the form of recursively learning, predictive recursion method. The convergence depends on a martingale construction and convergence of related series and heavily depends on the independence of the data. The general algorithm may not hold if dependence is present. We have proposed a novel martingale decomposition to address the case of dependent data.
Estimating the mixing density of a mixture distribution remains an interesting problem in the statistics literature. Stochastic approximation (SA) provides a fast recursive way for numerically maximizing a function under measurement error. Using suitably chosen weight/step-size the stochastic approximation algorithm converges to the true solution, which can be adapted to estimate the components of the mixing distribution from a mixture, in the form of recursively learning, predictive recursion method. The convergence depends on a martingale construction and convergence of related series and heavily depends on the independence of the data. The general algorithm may not hold if dependence is present. We have proposed a novel martingale decomposition to address the case of dependent data.
We select alpha1 and alpha2 that make the largest progress towards the global maximum value on each side of the hyper plane according to the heuristic function. The heuristic function is as follow: