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Essay On Regulating Breathing Within The Brainstem

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In recent years, substantial progress has been made toward understanding the mechanisms regulating breathing within the brainstem. Improved tools for vector design combined with molecular biology enable selective gene expression regulation to monitor and/or control over time cellular activity of targeted respiratory groups. These features are particularly important for studying respiratory centers in the brainstem since most nuclei are composed of heterogeneous populations of neurons, dynamically interacting with each other to generate and synchronize the respiratory drive. Indeed, several neuronal subtypes have been identified in the preBötzinger complex, the core of the neural circuit generating respiratory rhythm, which can be …show more content…

Using adeno-associated virus 2 for channelrhodopsin expression in specific preBötzinger neuronal subtypes in transgenic mice, it was demonstrated that pre-inspiratory preBötzinger neurons expressing developing brain homeobox 1 are primarily rhythmogenic. On the other hand, inspiratory preBötzinger neurons expressing developing brain homeobox 1 and somatostatin are playing a role in patterning the activity of rhythmogenic preBötzinger neurons and ultimately the respiratory motor output (Cui et al., 2016). Overexpression of enhanced green fluorescent protein driven by adeno-associated virus 2 with the somatostatin promoter in preBötzinger neurons revealed that these neurons project to several areas involved in the control of breathing, including contralateral preBötzinger, Bötzinger complex, ventral respiratory group, retrotrapezoid nucleus, parahypoglossal nucleus/nucleus of the solitary tract, parabrachial/Kölliker-Fuse nuclei and periqueductal gray (Tan et al., 2010). These projections support the modulatory role of somatostatin-expressing preBötzinger neurons on respiratory activity. Furthermore, it has been demonstrated that inhibitory glycinergic preBötzinger neurons exert significant contribution

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