Essay On The Injection Of The Drug

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Figure 23 [67]. Intracerebroventricular (ICV) injection of the drug to a mouse which is fixed in a stereotaxic frame. The drug will be injected to the right lateral ventricle of a mouse. For an alternative administration of the antiGAD-SHNNC-GAD-RITC, intrathecal (IT) injection allows delivery of the drug into the subarachnoid space. After anesthetizing the GAD65 -/- mouse, near the base tail hairs need to be shaved. The drug will be injected into the groove of L5 and L6 vertebrate by inserting a polyethylene (PE-10) catheter without injuring the spinal cord (Fig. 24) [68]. The mouse will be recovered after the pharmacological process. The same experiment will be implemented on the group of GAD67-/- and GAD65 & 67-/- mice to compare the…show more content…
Moreover, in a different set of negative control experiment a solution of free GAD65 and GAD76 will be administered after IN administration of antiGAD antibody as a negative control experiment. After one time IN administration of antiGAD-SHNNC-GAD-RITC, mice will be monitored for a period of 6 weeks to quantitatively investigate the drug sustainability. Figure 25 [72]. Intranasal (IN) administration of the drug to a mouse. The drug will be administered into the olfactory mucosa of a mouse. To determine whether the antiGAD-SHNNC-GAD is active after administration, the motor unit activity of all mice will be recorded by electromyography (EMG) device. EMG will demonstrate a higher rate of involuntary motor unit firing as well as continuous motor unit activity in control GAD65-/- mice due to lack of synaptic vesicular GABA as an inhibitory neurotransmitter. However, drug administered GAD65-/- mice will show normal motor unit activity (predicted result). In addition, to determine in vivo activity of antiGAD-SHNNC-GAD, mice need to be sacrificed and the amount of GABA can be determined by LCMS as described before (Fig. 26). We will also monitor neural activity and seizure development in GAD65-/-, GAD67-/- and GAD65.67−/− mice with electroencephalographic (EEG) recordings (telemetry system, IMT-100E, Star Medical, Tokyo Japan) following by analysis of their GABA-dependent emotional behavior, in
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