6.3 Experimental - schematic representation and procedure for the synthesis of compounds V1-17 6.3.1 Synthesis of 6-nitro-1H-benzo[d]imidazole-2-thiol II (step 1) Ethanol (40 ml) and potassium hydroxide (0.01 mol, 56.11 gm/mol, 0.56 gm in 2 ml H2O) were taken in a dry round bottom flask. 4-nitrobenzene-1,2- diamine I (0.01 mol, 153.14 gm/mol, 1.53 gm) was added to it and stirred well to get a clear solution. Carbon disulfide (0.02 mol, 76.14 gm/mol, 1.2 mL) was added to the clear solution obtained above and refluxed for 12 -15 h. The ethanol was distilled off and then cooled to room temperature. The content was poured into water and acidified with diluted HCl till the precipitates were separated. The separated solid was washed with cold water and dried to get the desired product. The completion of the reaction was monitored by TLC using ethyl acetate: …show more content…
°C % Yield Elemental Analysis % Carbon % Hydrogen %Nitrogen Calcd. Found Calcd. Found Calcd. Found V1 -H C15H12N4O3S 328 1420C 54% 54.87 54.85 03.68 03.70 17.06 17.09 V2 -4-NO2 C15H11N5O5S 373 1360C 83% 48.26 48.27 02.97 02.95 18.76 18.73 V3 -3-NO2 C15H11N5O5S 373 1400C 93% 48.26 48.29 02.97 02.99 18.76 18.75 V4 -2-NO2 C15H11N5O5S 373 1140C 92% 48.26 48.22 02.97 02.94 18.76 18.78 V5 -4-F C15H11FN4O3S 346 2100C 81% 52.02 52.06 03.20 03.23 16.18 16.15 V6 -4-Cl C15H11ClN4O3S 362 1940C 80% 49.66 49.68 03.06 03.04 15.44 15.47 V7 -3-Cl C15H11ClN4O3S 362 1820C 88% 49.66 49.63 03.06 03.08 15.44 15.42 V8 -3-Cl-4-F C15H10ClFN4O3S 380 2080C 86% 47.31 47.33 02.65 02.62 14.71 14.74 V9 -3-CF3 C16H11F3N4O3S 396 1980C 94% 48.49 48.51 02.80 02.83 14.14 14.12 6.4.1 Table-3: Physical constants data for derivatives V10-17 (Conti…) Derivatives Substituent Molecular formula Molecular weight gm/mol m.p. °C % Yield Elemental
The mass of the compound was 0.806 grams. This means that there was a percent yield of 80.6%. Looking at the table it appears that the synthesized compound is Ca(NO3)2. The synthesized compound has the same color for the flame test, the same pH level, and the mean value from the conductivity test was extremely
This Common Assessment Task assesses your ability to research and present information on a chemical element of your choice.
sterilized the loop by heat and tube streaked 26A and 26B on the agar and then incubated at 37
The sequences that should be mutated to make the pFLS2noWbox::GUS construct are the three W-box promoter motifs. The W-box promoter motifs are cis-regulatory elements found within the FLS2 promoter (pFlS2) that WRKY transcriptional factors recognize and bind to. These promoter motifs follow a specific consensus sequence, which defines a W-Box. The three sequences that should be mutated are: (+) TTGACC, (+) TTGACT and (-) AGTCAA. The addition of excessive mutations will guarantee that the promoter motifs will not be recognized by WRKY transcriptional factors. Therefore, by introducing 3 mutations into the middle of the three sequences, we can ensure that WRKY transcription factors will be unable to recognize and bind the three promoter motifs. Thus, the mutated sequences can be shown as: (+) TTAAAC, (+) TTAAAT, and (-) AATAAA.
It has an atomic abundance of about 0.012%; a half-life of 1.3 billion years; a specific activity of 2.6 × 108 Bq/kg; and a rounded dose conversion coefficient of 0.003 mSv/a per Bq/kg,
Most ADCs contain a complex arrangement of functional groups, both on the protein and the conjugated drug molecule. These functional groups vary in bond energy, electronegativity, hydrophobicity, and charge. Below in figure 2, the simplified general layout of a typical ADC can be seen.
My compound is baking soda that is its common name, Sodium hydrogen carbonate is its official name. Baking soda’s formula is NaHCOз. Some uses for baking soda is to make toothpaste, it is also used for cleaning and even baking purposes.The state of matter at room temperature is a solid and it weighs 2.2 g/cm³. Baking soda can be easily decomposed by acids, starting to decompose in water solution at approximately 20 degrees Celsius
I chose for my enrich topic for this week the subject of the "Synthesis of Urea". I chose this because I like the historical aspect of scientific discoveries. I immediately learned that when you do a Google scholar search for "synthesis of urea" that you get a number of results about modern patents on synthesizing urea. It was sort of difficult to find a historical article on the synthesis or urea in the scientific journal databases. I found a couple of articles from the 1920s that talked about Friedrich Wöhler, the man credited with the first synthesis of urea, "a naturally occurring organic compound from inorganic materials." ("urea". Encyclopædia Britannica. Encyclopædia Britannica Online. Encyclopædia Britannica Inc., 2016. Web. 26 Oct.
All solvents utilized in thin layer chromatography were distilled before use. 2.1. Experimental General Procedure for Thia-Michael Addition of Thiols to 2-nitro-3-phenyl-3-(phenylthio)propan-1-ol and its derivatives (6a-6f) Thiol 2.45g of 1 equiv (22.33 mmol) was added to a mixture of Nitroolefins 4g of 1 equiv (22.33 mmol) in DAPCO 2 equiv, and the mixture was vigorously stirred at room temperature until the nitroolefin was consumed completely (5
Ethanol (3.5 mL, ...M) was added to Benzoin (...g) followed by an addition of sodium borohydride solution to the suspension of Benzoin in ethanol. The mixture was then heated with swirling. After the benzoin in the mixture was dissolved, it was heated for further 1-2 minutes with occasional swirling. The hot solution was then diluted with water. The suspension was then chilled and the product was collected by vacuum filtration. The product was then further recrystallized from the minimum amount of hot ethanol to give colourless glistening plates. (....g, % yield), m.p.
The synthesized product was purified by further recrystallization process. The recrystallized salt was slowly added to the mixed solvent of benzene + acetone (1:2) ratio. The resultant solution was a pale orange colored. The solution was
acceptors and 1 hydrogen donor, whiles its molecular weight is 233.31 g/mol, well under the
collection and structure solution The composition of crystal Dimethomorph is confined by comparing the infrared spectra of two components. Crystal size of Dimethomorph is 0.30 x 0.20 x 0.20 mm The unit cell parameter of
THe After this, the reaction was terminated by adding 5% trichloroacetic acid. After the evaporation, the dry residue was dissolved in a mobile phase for HPLC(High-performance liquid chromatograph).
5 mole percent of Naphthalene to 95 mole percent of Biphenyl (which is good percentage of purity) has a broad melting range.