Extraction of Chlorophyll from Spinach Leaves

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The purpose of this experiment was to take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used, starting with the least polar, to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography.
The first part of the experiment dealt with breaking down the spinach leaves in a mortar and pestle. Acetone was added to this to help with the breakdown of the spinach leaves. Once the spinach leaves were broke down enough that you could see the particles
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The “E” solution ended up having the most spots because it was the pigment fragments. The Rf values could be calculated for all of the spots by taking the distance traveled by the spot and dividing it by the total distance traveled by the solvent front. The calculations are as shown:
Rf= distance traveled by spot (cm)distance traveled by solvent front (cm)
Tube E:
25.1= .39 (green) 4.25.1=.82(yellow) .55.1= .098 (yellow)
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