Extraction of Chlorophyll from Spinach Leaves

In the beginning of this experiment, our TA added water, salt, and 75/25 hexane/acetone to spinach leaves to a blender and blended the mixture to assume equal amounts for each group and to avoid erros if each student had to do the blending. The addition of the water to the mixture allowed the it to separate into a distinct organic layer after being run in a centrifuge, which was available to be collected at the top of the centrifuge. Salt reduces solubility, which will force the organic parts of

Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012   Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography, determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-

For the Plant Pigments I and II- Pigment Extraction lab, the objective was to find a solution that separated pigments and allowed them to travel up the chromatograph. The results of the experiment found that a 1:5 Acetone to Hexane ratio was the best solvent to use. In order to execute the lab we had to roll a penny over the spinach, which would in turn extract the spinach pigment. Even though this is how the experiment was carried, it was also a limitation (and will affect the outcome of the experiment)

INTRODUCTION Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. The photosynthesis process and their capability of producing organic materials from CO2 is having a big influence in the biosphere. The process of photosynthesis generates the plants to take two inorganic substances, CO2 and water, and also light, and remodel their atoms to make the organic compounds on which life depends: (McGraw-Hill) Light

Column Chromatography and TLC of Spinach Group Experiment 3 – Individual Lab Report (Save as pdf and submit, due by 12:00 NOON one week after experiment) Last Name: Wang First Name: Zoey Lab Partner(s): Sue Wang TA Name: Sosthene Date Lab Performed: Nov. 12, 2015 Date Lab Submitted: Nov. 12, 2015 Group A, B, or C: B Comments for Grading TA: (Please indicate if you performed the lab on a day other than your regularly scheduled day and/or with a TA other than your regular TA). Page Limit: report must

plants: carotenoids and chlorophylls. Under carotenoids, there are pigments called α and β-carotene and xanthophylls. Chlorophylls include chlorophyll a and chlorophyll b (Pavia et al 208). The main objective of this experiment was to extract these pigments from spinach leaves using chromatography techniques. First, the pigment was extracted from the leaves by column chromatography. However, the column was packed with granular anhydrous sodium sulfate. This first extraction acted as E or the main

Organic Chemistry 322 Lab Report Part I. Synthesis and Characterization of Aspirin Part II. Extraction of Natural Products Name: Bianca Davis Instructor: Dr. Russell Date Submitted: December 8, 2014   Introduction Aspirin is a substance known as acetylsalicylic acid. It is used mainly as an over the counter drug used to relieve inflammation, pain, and fever. Its natural form is salicylic acid. This synthesis process requires the acetylation of salicylic acid with acetic anhydride. The purpose of

tasty way. Juicing is usually done with a machine which turns raw fruits and vegetables into liquid and pulp. The machine chops the fruits and vegetables into tiny pieces and spins them, separating the juice from the pulp. History Even before juicers were invented fruits were mashed. Text from the Dead Sea Scrolls, "A pounded mash of pomegranate and fig" resulting in "profound strength and subtle form,” indicates that juicing has been around