Fluorescence Lab

K. Paraphrase the three potential fates of the excited electron produced when a photon meets a chlorophyll molecule

- I think that the electrons that are emitted from the cathode are emitted with a range of velocities (perhaps like a Boltzmann distribution where average speed clusters in the middle of the range).

The figure depicts the excitation of an electron into the conduction band thus leaving a hole in the valence band. An electron-hole pair is called an exciton, and the natural physical separation between them is called the excitonic Bohr radius and is characteristic of each material. Thus when a semiconducting material approaches a size nearing its Bohr excitonic radius, the exciton is said to be confined within the particle and is called quantum

Green fluorescent protein (GFP) comes from the jellyfish Aequorea Victoria is rare proteins with high fluoresce and absorbance. The purpose this experiments is to purify and express a His2-tagged recombinant from of GFP (rGFP) from the E. coli strain BL21(DE3)< pRSETA-GFPUV > through a series of experiments by using Ni+2 agarose affinity chromatography technology. The GFPuv gene (UV-optimized GFP) was over expressed in the E. Coil strain BL21 (DE3) (pLysS) as an n-terminal His6/Xpress epitope tagged bind protein. Then using Ni2+ Agarose affinity chromatography to obtain purification of the crude extract. Then observe under the long wavelength UV light, the activity of the rGFP in the column fraction. Bradford assay was performed to obtain the total protein amount. When calculating the

Every cell transports materials in and out throught something called a membrane. There are many different methods of transport in the cell Saccharomyces cerevisiae (Serrano, 1977) We want to know does adding higher concentrations of azide more effectively block dye transport? We tested the transport of dye in yeast cells with a metabolic inhibitor. When we did this we showed no difference in the absorbance between different azide solutions, and our control. From this we concluded that azide has no effect on the transport through a yeast cell membrane.

Flame Test Lab Questions: 1. What color of light is the lowest in energy? According to our physics class last year, the color with the lowest energy is red because it has the lowest frequency and the longest wavelength out of all colors. We can conclude that calcium chloride has the lowest energy.

Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify recombinant GFP using HIC. Once the protein is purified, it may be analyzed using polysaccharide gel electrophoresis (PAGE).

6) If the oscillation frequency of the transmitting electron decreases, the oscillation frequency of the electron in the receiver is instantaneously affected.

From the Photosystem II electron acceptor, the electrons are passed through a different electron transport chain. As they pass along the cascade of electron carrier molecules, the electrons give up some of their energy to fuel the production of ATP, formed by the addition of one phosphorus atom to adenosine diphosphate (ADP). Eventually, the electron transport carrier molecules deliver the Photosystem II electrons to Photosystem I, which uses them to maintain the flow of electrons to P700, thus restoring its function.

The importance of non-cyclic electron flow in the light independent phase is a light reaction which helps the excited electron pass

At the point when an atom loses an electron to edge a cation, the lost electron no more

In many materials, electron spins are equally present in both the up and the down

2. High frequency light causes electrons to be emitted from the sodium, however, a lower frequency light does not. 3. The energy of the emitted electrons does not depend upon the intensity (brightness) of the light, it is dependent on the frequency of the light.

The reasons for confinement can be electrostatic potential which can be due to external electrodes, strain, impurities and doping. According to a lot of research that has been going on in this field, a quantum dot has a discrete quantized energy spectrum.

With advent of scientific research and technological interest, multiferroic materials have drawn much attention for foundational physics, technological application in possible miniaturization and integration for multifunctional devices (e.g. magnetic field sensors, multiple state memory element, transducers, actuators, broadband magnetic sensors, non-volatile memory elements, oscillators, phase shifters, electric field controlled ferromagnetic resonance devices, switching devices, modulation of amplitudes, filters, waveguides, spin wave generation, energy harvesters, magnetic recording read heads, random access memories, RF resonators, tunable inductors, and ME antennas) [1-11]. The most intriguing characteristic of multiferroic materials