Follicle Simulation

Another commonly used method of induction is the injection of follicle stimulating hormones, or FSH. For this treatment, the patient is given FSH injections while being monitored by a physician. The physician uses ultrasound to keep count of the number of mature eggs in the woman’s ovaries. When the physician decides that there are enough eggs, a second hormone injection is then given that causes the release of the eggs from the ovaries. The final step is fertilization through sexual intercourse or artificial insemination (ACOG, 2004).

An alternative strategy for storing the female germ cells is cryopreservation of ovarian tissue. This method allows the storage of a large number of oocytes (within primordial follicles). Unlike fully grown oocytes, oocytes in primordial follicles tolerate cryopreservation very well. Several characteristics make them less vulnerable to cryodamage. The most important of these features are:

4. What is it called when an egg from the female is withdrawn and fertilized with sperm in a laboratory for 2 to 3 days with subsequent implantation into the uterus?

The majority of the process of keeping an embryo alive for an extended period of time is the same as in-vitro fertilization. “One day after retrieval (Day 1), the embryologist begins their day with the assessment of fertilization and by separating out the normally fertilized eggs. Cell division or cleavage will occur after the intermingling of the chromosomes overnight. By Day 2, normally dividing embryos should have four cells. Embryos will be further assessed on Days 2 and 3 for transfer or placement into more advanced blastocyst growth media until day 5 or 6”(AZFertility). However, in order to continue the growth, once the embryos are placed into the culture, they are placed into incubator. The majority of the difficulty is keeping the embryo alive within the

Gonadotropins are a group of hormones that are secreted by the pituitary gland that starts the production of the gonads. The reason gonadotropins are used in fertility is because it has FSH and LH hormone that aids in producing eggs in order for a woman to ovulate. So in other words, if a women does not have enough hormone to start ovulation she will be given a rFSH for about 12 days to help the eggs mature and then a dose of gonadotropin to start the ovulation process.

Eggs are removed from a female’s ovary and fertilized in a laboratory. The embryo which is the fertilized egg is transferred into a woman uterus to grow and develop. Progesterone supplementation is important for conception and staying pregnant after IVF, so, Crinone 8 gel may be used. A woman going through IVF requires more progesterone since in vitro process isn’t natural; you take medicines to produce multiple follicles and to prevent premature ovulation. Once the follicles have matured, human chorionic gonadotropin is administered to trigger ovulation, the eggs are harvested when the body least expects it; the whole process disrupts the natural function of progesterone hormone, this in an explanation of the need for the Crinone to support implantation and avoid a miscarriage. The gel is considered very effective since it’s readily absorbed into the bloodstream. Ensure you follow the prescribed dose, Crinone 8 gel instructions and discard the applicator after

Have questions about in vitro fertilization? Come to IVF 101 at our DFW fertility center to get answers.

Ovation Fertility™ Newport Beach laboratory director discusses development of assisted reproductive technologies in latest paper

An advanced reproductive instrument is used this is called In-Vitro Fertilization or IVF. This device

After fertilizing the eggs, the male closely controls the prenatal environment of the embryos in his pouch. The male keeps blood flowing around the embryos, controls the salt concentrations in the pouch, and provides oxygen and nutrition to the developing offspring through a placenta-like structure until he gives birth” (science.tamu). The male member of the partnership then fertilizes and carries the eggs for a range of the ten days to forty two days, a length of time dependent on the species and water conditions (SFSU Geography and Dames N 2000). Upon conclusion of this gestation period, the male carrier begins the process of live birth, a moment that is usually held off until the ocean tide is near its peak (SFSU Geography and Dames N 2000). This waiting period ensures food and potential security for the newborn Syngnathids: fully developed offspring who receive no additional care from the parents and must learn to forage and survive on their own (SFSU Geography and Dames N 2000). This course of action will likely lower the survival rates of the newborn offspring, but also enables the parents the continue onto another cycle of mating and reproduction (SFSU Geography and Dames N

This medication allowed for the gonadotropin hormones to be repressed so that premature ovulation could stop. Once ovulation ceased, the patient was then subscribed Follistim Injections for daily use for 2 weeks. During these couple of weeks, the client was scheduled for multiple ultrasound visits to monitor the size of the ovum that were being produced. After those two weeks passed, Subject 1 eggs were of a mature size and was administered an injection of Ovidrel (Human Chorionic Gonadotropin) under the skin. This injection caused the release of the egg and egg retrieval was scheduled for two days from the current

An egg at each developmental stage (24, 28, 72, and 96 hour) were obtained with their blunt end positioned up because the embryo will be sitting just below the air space, while the yolk gravitates to the bottom. Each egg was opened from the blunt end by removing the shell membranes with forceps and scissors. The embryos of each stage were taken out by a technique that consisted of the embryo attaching to a filter paper, and then getting cut around the ring with scissors. The embryo was transferred to a small petri dish with Howard’s Ringer’s solution. Each embryo was examined and compared to one another.

The procedure starts at the embryology laboratory where the embryologist will collect the embryos from the petri dish using a catheter which is connected to a syringe. At the time of collection the embryologist should make sure that the embryos are kept close to each other inside the catheter. When the embryos are inside the catheter, a small amount of air is also absorbed to ensure that they will remain in the catheter. The embryologist will then transfer the catheter carrying the embryos to the doctor inside the surgery room. The doctor will insert the catheter inside the vagina using a guide which will help him to reach more easily the uterus. Once the catheter has reached the uterus, the doctor will liberate slowly the embryos. The catheter will be checked again to make sure that all the embryos have been transferred. If the catheter is clear it means that the procedure is over. Twelve days after the embryotransfer the woman should perform a pregnancy test which will determine if there is a pregnancy or

• Prepare the pituitary gland prior treatment with fertility shots to induce ovulation in assisted reproductive technologies such as in-vitro fertilization

I would like to explain some of the topics that I choose which is the in vitro process start with in vitro maturation that means the significant numbers of immature oocytes can be collected from ovaries and mature them in the laboratory without recourse to ovarian stimulation. Besides that, the selected oocytes are then incubated for a period of 24 hours, this is called the maturation period. This involves artificial removal of cumulus-oocytes complexes (COCs) from antral follicles and culturing them in essentially standard cell culture condition until they reach maturity or metaphase II stage. From my view, this method reduces the time, risks associated with conventional IVF, and costs.