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Glyphosate And Roundoup Lab Report

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Blepharisma Unaffected by Glyphosate; Killed by RoundUp

INTRODUCTION

Blepharisma (Blepharisma japonicum) is a protist found in fresh and saltwater. Runoff is a common problem in lake water, particularly RoundUp. The active ingredient in RoundUp is glyphosate. Glyphosate works by blocking the synthesis of proteins in certain plants, subsequently killing them (National Pesticide Prevention Center, 2010). Because of this finding, it was assumed that glyphosate would have a similar effect on microbes. The purpose of this experiment was to observe the effect of different concentrations of glyphosate and RoundUp on blepharisma.

The goal was to examine the environment found in Wisconsin lakes and measure the effect that runoff has on microorganisms in nature. The hypothesis of the experiment was that if glyphosate is added to the environment then blepharisma will move across the field diameter slower.

METHODS

Preparing Glyphosate and RoundUp Solutions

Solutions of glyphosate and RoundUp were prepared at several concentrations. The concentrations of glyphosate made were 1%, 25%, 50%. Glyphosate solutions were created by adding concentrated …show more content…

By determining how long it took one organism in the control condition (no glyphosate or RoundUp added to sample) to cross the field diameter and dividing that time (in seconds) by the approximate field diameter (~4mm), the control speed was determined to be approximately 1.97 mm/s (4mm/7.89 s).

Testing the Effect of Glyphosate and RoundUp on Blepharisma

To test the effect of glyphosate and RoundUp on Blepharisma, two drops of one solution from a transfer pipet, at a single solution at one concentration, were added to the sample on the glass slide. The dependent variable was organism speed, so the effect was measured in terms of the change in speed of blepharisma as the moved across the field diameter.

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