Blepharisma Unaffected by Glyphosate; Killed by RoundUp
INTRODUCTION
Blepharisma (Blepharisma japonicum) is a protist found in fresh and saltwater. Runoff is a common problem in lake water, particularly RoundUp. The active ingredient in RoundUp is glyphosate. Glyphosate works by blocking the synthesis of proteins in certain plants, subsequently killing them (National Pesticide Prevention Center, 2010). Because of this finding, it was assumed that glyphosate would have a similar effect on microbes. The purpose of this experiment was to observe the effect of different concentrations of glyphosate and RoundUp on blepharisma.
The goal was to examine the environment found in Wisconsin lakes and measure the effect that runoff has on microorganisms in nature. The hypothesis of the experiment was that if glyphosate is added to the environment then blepharisma will move across the field diameter slower.
METHODS
Preparing Glyphosate and RoundUp Solutions
Solutions of glyphosate and RoundUp were prepared at several concentrations. The concentrations of glyphosate made were 1%, 25%, 50%. Glyphosate solutions were created by adding concentrated
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By determining how long it took one organism in the control condition (no glyphosate or RoundUp added to sample) to cross the field diameter and dividing that time (in seconds) by the approximate field diameter (~4mm), the control speed was determined to be approximately 1.97 mm/s (4mm/7.89 s).
Testing the Effect of Glyphosate and RoundUp on Blepharisma
To test the effect of glyphosate and RoundUp on Blepharisma, two drops of one solution from a transfer pipet, at a single solution at one concentration, were added to the sample on the glass slide. The dependent variable was organism speed, so the effect was measured in terms of the change in speed of blepharisma as the moved across the field diameter.
his experiment showed the effect of the active ingredients of the weedkiller Roundup and the effect that it has on Daphnia at different water temperatures. At the conclusion of this experiment it was found that the active ingredients in the weed killer did indeed have an effect on the Daphnia but 26 degrees Celsius its effectiveness doubled
The purpose of this course is to provide the fundamental knowledge of the effects of environmental chemicals on living systems, and the toxic responses of the human and plant systems. Students will discuss risk, ethics, and social responsibility with regard to environmental toxicology.
which is an herbicide known as “Roundup.” It is non-selective, which means that it kills any plants
King’s College London is the college that started all the commotion about Monsanto’s herbicide Roundup being a concern with damaging organs such as the liver and kidneys. They did a science experiment with rats. They found that rats that were exposed to just a tiny amount of Roundup in their
crops led to the rise of “superweeds,” which are resistant to the key active ingredient in
Duke, S. O. (1995). Herbicide-Resistant Crops: Agricultural, Economic, Environmental, Regulatory, and Technological Aspects. 83-84. Boca Raton, Florida: CRC Press.
However, all that Round up residue doesn’t just wash of the crops. Then we put harmful chemicals into our body. Why would the U.S. fall into the Trap of Monsanto? Countless other countries like the UK have entirely banned Monsanto. Apparently the U.S. fell into what Monsanto wants us to think.
Bt [proteins] have been tested against a wide variety of terrestrial and aquatic invertebrates including earthworms, collembola, daphnids, insect predators and parasites, spiders, and honeybees and have been shown to have a high degree of safety for these non-target organisms. In most cases, no adverse effects were observed even though test populations were exposed to levels of toxin in excess of 500-1,000-fold concentrations than they would be expected to encounter under field conditions.
Howard, P.H. (ed.). 1989. Handbook of Environmental Fate and Exposure Data for Organic Chemicals, Vol. III: Pesticides. Lewis Publishers, Chelsea, MI.
The Great Lakes, though fairly clean, are contaminated with some deadly chemicals that have slipped by human filtration into the lakes. These emerging chemicals have been determined to pose some health risk to humans and surrounding ecosystems and are used by society, but are unregulated or inadequately regulated retardants (Miller-Branovacki, Lindsay, "Water Resources and Urban Waste Water (Week #8)"). More specifically an emerging contaminant is one which is not historically widely distributed, nor a concern, however are now persistent and bioaccumulative making them a concern for the environment. The three major categories for emerging contaminants are; endocrine disrupting compounds (EDCs), Pharmaceutical and personal care
A product manufactured by Monsanto is the weed killer Roundup Ready 2 Xtend. The Roundup Ready 2 Xtend is used to by soybean farmers and claims to provide growers with an outstanding performance with their efforts to produce the best crop possible. However, the product is not currently approved by the Environmental Protection Agency. Farmers will like the new roundup ready plus because it will give them a higher yield on their crops. Monsanto claims that the farmers can expect up to four bushels of soybeans per acre (Monsanto, 2016). In order for the new Roundup Ready 2 Xtend to be used in the United States, each state has to approve the use of the product. The product is used by spraying it onto the crops in order to combat pests.
pesticides, cholecalciferol (Vitamin D3), phosphorus, pindone and brodifacoum, are even less successful then cyanide in comparison to 1080. Phosphorus has no antidote and is considered inhumane, causing painful deaths to animals. Cholecalciferol is expensive and secondary risks are unknown. Brodifacoum is a slow working pesticide taking 1-2 weeks to kill pests and persists in the environment. Pindone is even slower working taking 2-3 weeks to kill poisoned pests and persists moderately in the environment. 1080 is the most effective pesticide due to the lack of better alternatives and whilst 1080 is dangerous it is clear the other options pose even higher risks to humans and other non-pest species. For lack of better alternatives, 1080 needs
By getting rid of the heritage plants, many of the crops lose many of their nutrients which are replaced by an array of toxins and GMO seeds. According to AgMag, big GMO companies like Monsanto, claimed the use of genetically modified crop were going to increase crop yields. Referring to the “agricultural output” (Staff), in other terms, the amount of a crop that was harvested per unit of land. Instead, the crops were not improved and there was a noticeable increase in the use of glyphosate; as known as, “Glyphosate is an herbicide. It is applied to the leaves of plants to kill both broadleaf plants and grasses. The sodium salt form of glyphosate is used to regulate plant growth and ripen fruit” (Ewg) according to the National Pesticide Center. As the graph below shows, the tremendous increase in the use of Glyphosate used on wheat. In the year 1999, there was approximately 4,000 pounds being dumped into the crops. Yet it did not stop there, as in 2009, the amount of glycophase spiraled upwards to an astonishing sixteen thousand
DDT (dichloro-diphenyl-trichloro-ethane) was one of the most commonly used pesticides during the mid-1900's. This chemical was found to be effective in killing mosquitoes, thus stopping malaria epidemics, and was also useful against farm pests. However, mosquitoes often became resistant to this unnatural chemical after a generation or so of selective breeding. In fact, this pesticide did much more than simply getting rid of the insects pestering man. The most deadly feature of DDT was its ability to move through the food chain at a deadly pace.
The use of herbicide to maintain crops has been an unchanging figure within our farmlands, within the growing of our own food. The pumping of this pesticide isn’t only harmful towards the weeds that we wish to exterminate, but these toxins also harm our own children and animals. Texas A&M University stated, “the exposure to herbicides can produce mild symptoms, such as rashes, nausea and headaches; more dangerous symptoms, such as seizures and convulsions; and even death.” There are different ways to distribute this harmful substance, such as through a liquid base, powder, and even by premixing into fertilization products. The Department of Veterans Affairs confirmed that chloracne, which is a form of acne, is associated with Agent Orange. Agent Orange was used during the Vietnam War as a chemical weapon against the Vietnamese, the U.S. military sprayed millions of gallons of Agent Orange across Vietnam, from 1961 to 1971,