Results: One result of this experiment was a standard curve of the density of yeast cells in relation to absorbance (Figure 1). We found that as the density was increasing, the absorbance was increasing (Figure 1). We used the equation of the standard curve from Figure 1 to determine the density of cells at different time intervals by multiplying the slope of the line from Figure 1 (y=16.691x) with the absorbance level we had measured for each solution. The density (cells/mL x 10^6 was then plotted against the time intervals (Figure 2). The yeast grew faster in the 5.0pH environment since the density nearly doubled in the same time it took the yeast from the 2.3pH environment to increase by ½ the original density (Figure 2). Also, the yeast …show more content…
The yeast grew faster in the environment with a pH of about 5 (Figure 2). Some evidence of a carrying capacity was shown (Figure 2). The yeast grown in the pH 2.3 environment had a carrying capacity of about 0.308 (Figure 2). However, there was no definitive evidence of a carrying capacity for the yeast grown in the pH 5 environment since the population did not stabilize (Figure 2). It is possible that the carrying capacity for that environment is about 0.828, but more tests would need to be done to solidify the legitimacy of that estimate (Figure 2).
The results of our experiment mirror the results of Heard and Fleet’s experiment. They determined that different yeast species excel in different pH environments. They found that one species, Saccharomyces cerevisiae, particularly excelled in a pH environment between 3.0 and 3.5 (Heard & Fleet, 1988). The yeast used in our experiment thrived in a similarly low pH environment of about 2.3 (possibly higher depending on the true carrying capacity of the yeast in the pH 5 medium) (Figure 2). Our species of yeast may overpower other yeast species if placed in a more competitive environment. However, not all experiments agree with ours. An experiment by Fleet, Charoenchai, and Henschke resulted in no significant changes in growth rates in wine yeast population between medium pH environment levels of 3.0 and 4.0 (Charoenchai, 1998). Our results do not prove a significant difference,
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Creating the best possible environments for yeast growth can help increase culinary yeast production. Growing yeast in an environment with a pH around 5 will increase production as opposed to growing yeast in an environment with a pH around 2.3 (Figure 2). This can increase revenue for many companies. In addition, medical professions can benefit from this experiment. Knowledge of which environments encourage yeast growth will help create remedies for yeast related afflictions. Forcing yeast to live in a more acidic environment, such as a pH of 2.3, will discourage growth as opposed to a location with a higher pH (such as 5) (Figure 2). All in all, there are many real-world applications for this
The experiment was conducted to determine the impact different yeast amounts had on yeast fermentation. It was hypothesized that the more yeast added the more CO2 would be produced. The carbon dioxide production was measured in the fermentation of yeast with solution of no yeast in test tube 1, 1mL yeast in test tube 2, and 3mL of yeast in test tube 3 over a period of twenty minutes. All of the yeast amounts produced CO2, but test tube 3 was the most efficient of the three.
The aim of this experiment was to see how temperature affected the rate of fermentation. To test this a yeast and glucose solution was submerged in water baths with the temperatures of 20oC, 30oC and 40oC. The test was left over night to gain optimum results. The rate of fermentation was measured by the amount of carbon dioxide produced. It was believed that the solution submerged in the 40oC water bath would produce the most carbon dioxide and therefore ferment the fastest. Results showed that the hypothesis was correct as after 25 hours the solution submerged in the 40oC water bath had produced the most carbon dioxide.
PH can affect the way fermentation occurs due to the irregularity of the acidity or alkalinity within the glucose solution. This is an enzyme-based reaction that is susceptible to pH. The aim of this experiment was to determine how pH affects the yeast fermentation rate by performing the experiment numerous times with a different pH of glucose solution which included pH 3, 5, 7, 9, 11. The hypothesis was ‘If the pH is lower than the neutral point then the fermentation reaction will occur faster?’ The experiment conducted was to measure the amount of C02 produced by the yeast going into fermentation, however varying the pH of glucose solution by using different pHs . To test this every 5 minutes the volume of gas in the test tube was observed and recorded until a period of 30 minutes had been. The end results
Salinity - All yeast spheres will be soaked in the same water, with no salt added
In the S. cerevisiae specimen, moderate growth was expected and observed based on the knowledge that most microbes desire this type of environment for growth. The same was expected although a larger amount of growth was observed for the S. epidermidis specimens in the 1% NaCl solution than expected. The 1% NaCl solution provided the best environment for the growth of both microbes. Minimal growth of S. cerevisiae and moderate growth of S. epidermidis was observed from the 7% NaCl solutions. The S. epidermidis is used to a slightly salty environment on the surface of skin which may account for the higher growth over S. cerevisiae in this environment. Lastly, no growth was noted in either specimen of 15% NaCl. This type of environment does not support the growth of most microbes due to the increase in salt content and the hypertonic environment it creates.
Abstract: This lab’s purpose was to see how different levels of yeast, distilled water, and sugar interact to affect the level of carbon dioxide evolved in fermentation. In this experiment we had two sections. The first section tested four test tubes with varying levels of yeast, glucose and distilled water for evolved carbon dioxide levels. The tubes were timed for 20 minutes. The amounts of solution in the test tubes are noted in the methods section of this lab report. The second section of the lab used three test tubes and flowed the same procedure except added spices. The levels of ingredients are also in the methods section. The main goal of this experiment was to see the effects of yeast concentration.
In this lab we tried to find what fuels yeast could metabolize and what the yields of the carbon dioxide gas that were produced from the different sugars used. We used 6 different yeast and sugar mixtures. The different yeast and sugar mixtures we used were control, glucose, sucrose, fructose, starch, and saccharin. The results for the 6 different results are presented in Tables 1-6 and Graph 1. Graph 1 is a graph of all the information in Tables 1-6. Each Table and graph is labeled approximately.
1. Gathered all required materials to designated lab bench. 2. Considered all safety precautions including the prevention of spilling water to avoid falls, handling glassware carefully to prevent shattering, avoiding long periods of working with warm water to avoid burns and avoiding the digestion/inhalation of by-products produced after the reaction (e.g. ethanol and carbon dioxide gas). 3.
The reaction taking place is catalase breaking down hydrogen peroxide into oxygen and hydrogen. The enzyme is catalase, the substrate is hydrogen peroxide, and the products are hydrogen and oxygen. The question being tested is when we increase the yeast concentration, what will happen to the rate of the reaction. If increasing yeast concentration affects the rate of reaction, then the time it takes the disc to rise will decrease.
Fermentation- Required bacteria is grown at at 30°C in a stream of air in a mineral medium with glucose as a carbon-source. As a result, the cells multiply after 24 hours and reach a higher cell density. This is known as
Higher levels of solution should produce higher levels of product. The independent variable for the control group data and the experiment data is the yeast concentration. The dependent variable for the control group data and the experiment data is how much oxygen is produced. The Constant for the control group data and the experiment data is time and amount of hydrogen peroxide. The products of the experiment will increase if the levels of reactants increase. Denatured yeast may cause change in the reaction of the experiment. For all trials of the control group, the concentration of yeast is 6ml. For the experiment data, the yeast concentration varies from 8mL, 10 mL, 12 mL, 14 mL, and 16 mL. The temperature may cause change in the reaction of the combination of yeast and hydrogen peroxide
For the experiment, the changes of temperature on anaerobic fermentation the process in which cells undergo respiration without oxygen in Saccharomyces cerevisiae was observed. The purpose of this experiment was to test the effect of four different temperatures on the rate of carbon dioxide production in yeast by measuring the fermentation rate. Saccharomyces cereviviae, also known as Baker 's yeast, is a unicellular, eukaryotic sac fungus and is good for this experiment because of its characteristic of alcohol fermentation. It was hypothesized that fermentation increases with increased temperature to a point of 37°C; above that point, enzyme denaturing will occur and fermentation will decrease. The group was able to document the carbon dioxide production and mark each of the temperature intervals which were tested at temperatures 4°C (refrigerator temperature), 23°C (Room temperature), 37°C (Human body temperature) and 65° Celsius (Equal to 150°F). The experiment was conducted by pouring yeast solution with 2% glucose in fermentation tubes, placing the tubes in the appropriate incubation temperature, marking the rise of the gas bubbles in the fermentation tubes which indicated carbon dioxide production. The results of this experiment were not supported by the hypothesis, creating different results from what was predicted. It is important to understand the fermentation rate of yeast so
Hypothesis: If the mass of yeast (g) is increased the rate of fermentation of glucose (mL/s) will increase.
In order to study the evolution of yeasts, they must be captured and propagated. The process for doing so is rather simple; a juice or slightly sweetened drink, such as a tea, is left open to the air with a loose filter, such as cheesecloth, for a day or two. Once signs of
Six experiments were carried in this report concerning the effect that different environmental factors have on microbial growth. The results were recorded into tables where (+) symbolises growth and (–) symbolises no growth.