Measuring Cytotoxicity on Cancer Cells by Inhibiting the Reduction of 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide to generate NADH and NADPH
Abstract: Chemotherapy is a well-known treatment when it comes to treating cancer, however the drugs are often non-specific and can end up damaging perfectly healthy cells, leaving the patient in agony, or not achieve anything. In this study we looks into the area of personalised medicine where different drugs are used for different patients with the same cancer dependent upon the effects this drug will have. Two chemotherapeutic agents, named A and B, were used to prevent the cell proliferating and therefore stop the cancer progressing and surviving as the cells starve to death. A
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Radiotherapy was the first method non-surgical method used to cure cancer around 100 years ago and was, up until recently, the most common way to do so (http://cancerres.aacrjournals.org/content/68/21/8643.full.html). The basis is simple, an ionising beam is fired at the tumorous cells to alter those cells DNA. The mutations are fatal and the cells die, therefore curing the cancer, however many healthy cells around that tumour also get mutated and can also die in the process so there can be some severe side effects from radiotherapy. Chemotherapy was invented around 40 years later (same guy unfortunately to link) in 1935 but due to lack of progress it was dismissed until the 1960’s when there was proof that it actually could cure cancer and not just relieve certain symptoms but do nothing else. Whilst radiotherapy is still the most common treatment of cancers chemotherapy is now a treatment almost everyone will have heard of and is a growing field in an attempt to cure cancer with various drugs while minimising the side effects some of these drugs can have. However vary rarely are these treatments used as standalone treatments nowadays, combining the above techniques in different ways to ensure total removal of the cancerous cells. This method is called
According to the oral cancer foundation, “Chemotherapy is the use of chemicals to destroy cancer cells. Chemotherapy works by interfering with the cancer cell's ability to grow. It is one of the three main methods utilized to treat cancer.” With cancer being a potentially fatal disease it is important to diagnose it as soon as possible. the oncologist have to study the chemicals used so that they can determine what chemicals will treat the cancer or disease the best and what chemicals can be mixed together without any major side effects. The drugs can be divided into groups based on how they work, their chemical structure, and their relationship to other drugs.
Radiation therapy is commonly used as a proven and widely accepted way to treat cancer. The American Cancer Society attests to this because of the extensive research they have done in this field. High-energy radiation damages a cell’s deoxyribonucleic acid (DNA). When a cell divides, its DNA splits. According to the Mayo Clinic, radiation therapy is sometimes given with curative intent (that is, with the hope that the treatment will cure cancer, either by eliminating a tumor, preventing cancer recurrence, or by both). In such cases, radiation therapy may be used alone or in combination with surgery, chemotherapy, or all three. Radiation therapy may also be given with palliative intent. Palliative treatments are not intended to cure. Instead, they are meant to relieve certain symptoms and reduce
Treatment: As long as the cancer has not spread to outside of the lung, it can be removed with surgical resection. There are now less invasive ways to remove cancer from the lungs, such as using a small video camera to see inside the chest, resulting in smaller incisions. There has very recently been a new way to treat non small cell lung cancer that would otherwise be inoperable. Shown to be more effective than normal radiation therapy and simply observing the cancers progress, stereotatic body radiation therapy can ???. Laser treatment is sometimes used to unblock air passages that are blocked by a tumour and attempt to control some spreading of the cancer cells. It is different to the other ‘treatments’ as it doesn’t actually provide a solution to getting rid of the cancer, but makes the symptoms more bearable for the patient.
They may be applied to bloodstream or directly to a cancer site. Chemotherapy typically refers to the destruction of cancer cells, however some may include antibiotics or other medications to cure an illness or infection. Often, chemo is used alongside other treatments. Physical activity is important and it’s been found that patients who regularly exercise after chemotherapy treatment have a much lower risk of cancer recurrence. Chemotherapy generally has 5 goals; total remission, combination therapy, delay/prevent reoccurrence, slow down cancer progression and relieve cancer symptoms. The objective is to prevent cell division using cytotoxic drugs, to target cancer cells food source, enzymes and hormones that they need in order to grow, and to stop the growth of new blood vessels that supply the tumor. Patients can either receive monotherapy where they’re just given one drug, or combination where the patient receives more than one drug. These courses of chemo can be just a one day treatment or last for a few weeks, it depends on the type and stage of the cancer basically how advanced it
cancer cells, either by killing the cells or by stopping them from dividing. Radiation therapy is a treatment that uses high-energy x-rays or other types of radiation to kill cancer cells or keep them from growing. Immunotherapy is a treatment that uses the patient’s immune system to fight cancer. Lastly targeted therapy, is the treatment that uses drugs and other substances to attack cancer cells. Some of these treatments are standard and others are being clinically tested in clinical trials. Patients may enter clinical trials before, during, or after starting their cancer treatment. Follow-up tests may be needed.
Chemotherapy is the administration of chemicals into the body in an attempt to cure/lessen the severity of cancer in living organisms. Chemotherapy is always given with a curative intent, but that cannot always be promised to the patient, so sometimes it is given to relieve symptoms that the patients are experiencing or to improve their overall quality of life.1 While chemotherapy is used as an agent to try to rid individuals of their cancer, it is essentially slowly killing them. The chemicals used for the treatment are toxic to the human body and while they are giving them in small amounts, those small amounts accumulate over time to large amounts in the body. The body is affected by the treatment in various different ways and every body
There are clinical trials that are working through new chemotherapy regimens, precision medicine or targeted therapy, ALCHEMIST, AGFR, angiogenesis inhibitors, cryotherapy, and IGRT. Surgery includes pneumonectomies, segmentectomies, lobectomies, and thoractomies. A pneumonectomy is the removal of the entire lung; a segmentectomy is the removal of a small segment of the lung; a lobectomy is the removal of a lobe of the lung; and a thoracotomy is the removal of cancerous lung tissues. In some cases, chemotherapy will follow up a surgery for lung cancer. Chemotherapy is the use of drugs to treat a disease. With cancer, there is adjuvant chemotherapy and preoperative chemotherapy. Adjuvant is after surgery while preoperative is before surgery. If a patient is of advanced age, or has other medical conditions that will make it harder for the patient to undergo surgery, they can have radiation done. Radiation uses high energy particles or waves to damage cancer cells. Lastly there is a combination of these techniques. A patient can have radiation before a surgery, chemotherapy before a surgery or after, or a patient can have a combination of radiation and chemotherapy. A patient can pick any way they would like, but the choice needs to look at how the patient is doing, what stage they are in, any other medical problems, and how they want to feel going through all of
Through many cancer researches in the last decades, it was found that cancer cells often use glucose more voraciously and quite differently from normal cells. Therefore, many researchers and pharmaceutical companies have thought that the nutrient supply and deprivation of the cancer cells will potentially be the next target in disrupting the metabolism of cancer cells.
Chemotherapy is a very term used to describe a wide variety of several different ways to treat cancer. "Chemotherapy is treating cancer with drugs. But this is different from most kinds of drug therapy. Drugs that kill cancer do affect the rest of the patient's cells. Doctors try to work with what makes the cancer cells different to find ways to kill the cells without harm to the rest
Surgery is used to efficiently remove parts of the body containing cancerous cells. This includes the tumours, normal tissue around the tumour and lymph nodes. If they didn't remove the normal tissue that surrounds the tumour and it contained cancerous cells, then the cancer could redevelop. Chemotherapy is a form of drug therapy that uses either one type or numerous types of chemotherapy drugs combined to slow down the growth of cancer cells and eliminate them. Other types of drug therapy include hormonal therapy, biological therapy and targeted therapy. Radiation therapy focuses high concentrations of radiation onto the area containing cancer cells to damage them. This treatment has daily sessions to repeat the process, the cancer cells don’t heal before the next treatment and they die, while normal cells aren't affected because they are able to repair. Since radiation can permanently damage healthy cells, doctors must use a precise amount of radiation to destroy the cancer cells, but leave normal cells intact.
The National Cancer Institute states that radiation therapy, “uses high-energy radiation to shrink tumors and kill cancer cells.” Radiation uses X-rays and gamma rays to shrink the tumors. Because cancer is rapidly growing cells, the cells need to be shrunk. By using radiation, the cancer cells’ DNA gets damaged, when the cell is damaged enough, it will stop growing and eventually die. The negative side to radiation is that it may also damage normal and healthy cells. Like chemotherapy, radiation can be given out in different doses depending on the type of cancer, size, location, etc. Besides the common treatments like radiation, there are newer, effective treatments like
Therefore, dose and duration of treatment is limited, which in turn limits the amount of normal and tumor cell death. A second mechanism is the suppression of cancer cells for variable periods of time without cell death. This mechanism is referred to as remission. Unfortunately, the cancer can return at any time, and it is sometimes stronger. Here another limitation is introduced. Some tumor cells can develop resistance to a particular chemical agent, or several chemical agents, limiting the types of chemotherapeutic agents available for effective use. The last mechanism is cell differentiation, which helps the immune system learn to recognize and fight tumor cells (1).
According to the Office of National Statistics, in 2013, 29% of deaths in the UK were cancer related, making it the leading broad cause of death in the population. In the United States, 1 in 4 deaths are cancer related, and it is estimated that in a lifetime, there is 44% chance of contracting an invasive cancer if you are male, and a 38% chance if you are female (Siegel et al. 2014). So, although our knowledge and education into different cancer mutagens such as UV radiation, chemical carcinogens and viruses (Alberts et al. 2002), as well as cancer physiology and treatments may have improved, leading to a decrease in cancer related mortalities, there are still huge advances to be had in the field (CRUK 2013).
In 2007, it is predicted that almost 1.5 million people will be diagnosed with cancer in the United States (Pickle et al., 2007). More than half of these cancer patients will undergo the use of radiation as a means for treating cancer at some point during the course of their disease (Perez and Brady, 1998). Cancer, a disease caused by an uncontrollable growth of abnormal cells, affects millions of people around the world. Radiotherapy is one of the well known various methods used to treat cancer, where high powered rays are aimed directly at the tumor from the outside of the body as external radiation or an instrument is surgically placed inside the body producing a result of internal radiation. Radiation is delivered to the cancerous regions of the body to damage and destroy the cells in that area, terminating the rapid growth and division of the cells. Radiation therapy has been used by medicine as a treatment for cancer from the beginning of the twentieth century, with its earliest beginnings coming from the discovery of x-rays in 1895 by Wilhelm Röntgen. With the advancements in physics and computer programming, radiation had greatly evolved towards the end of the twentieth century and made the radiation treatment more effective. Radiation therapy is a curative treatment approach for cancer because it is successful in killing cancerous tumor cells and stop them from regenerating.
Radiotherapy is a is a method of treating a particular disease, most commonly being a type of cancer. To do this, different forms of radiation are used, such as X-rays, gamma rays, electron beams or protons. Whichever form of radiation is used then forms a focused beam of energy and sends it directly to the cancerous cells, while minimising exposure to