Mic Final Exams...

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AS/OCT2010/MIC455

UNIVERSITI TEKNOLOGI MARA FINAL EXAMINATION

COURSE COURSE CODE EXAMINATION TIME

GENERAL MICROBIOLOGY MIC455 OCTOBER 2010 3 HOURS

INSTRUCTIONS TO CANDIDATES 1. 2. This paper consists of ten (10) questions. Answer ALL questions in the Answer Booklet. Start each answer on a new page. Do not bring any material into the examination room unless permission is given by the invigilator. Please check to make sure that this examination pack consists of: i) ii) the Question Paper an Answer Booklet - provided by the Faculty

DO NOT TURN THIS PAGE UNTIL YOU ARE TOLD TO DO SO
This examination paper consists of 5 printed pages
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(4 marks) What is the use of negative staining? (2 marks) c) Most genera of bacteria will lose the red carbolfuchsin stain when decolourized using acid alcohol but those that are "acid-fast" retain the bright red colour in ZiehlNielsen Acid-Fast stain. What could be the explanation for this colour differences. (3 marks) You are given a bacterial sample and your task is to find out whether the bacteria are motile or not. Suggest three (3) lab procedures that enable you to test on the bacterial sample. (3 marks)

b)

d)

QUESTION 8 a) List down three (3) advantages of transformation. (3 marks) b) Describe the mechanism of conjugation with the aid of a labeled diagram. (6 marks) c) What is the main difference between conjugation and transformation? (2 marks)

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AS/OCT 2010/MIC455

QUESTION 9 Discuss in detail the importance of fungi in different fields : agriculture, industrial and medicine. (10 marks)

QUESTION 10 Figure 1 demonstrates the patterns of oxygen used by different organisms incubated for 24 hours in tubes of a nutrient broth.

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Identify the possible group of organism that accumulate in tubes A to E as

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