Optogenetic Synthesis

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Optogenetics is a recently developed class of techniques, in which light-sensitive proteins can be used for controlling the activity of cells, such as neurons 1. Prior to the current study: “Optogenetic control with a photocleavable protein, PhoCl”2 there were three categories of optogenetic tools: light activated channels and pumps based on microbial opsins, proteins subject to light-dependent allosteric control, and proteins that have light-dependant changes in oligomeric interactions. The current study2 will address a newly developed fourth category of optogenetic tool, a photocleavable protein, PhoCl. The study explains how it was developed and improved in addition to explaining how PhoCl can be applied to control a cell’s activity2. The author hypothesizes that PhoCl is a valuable new class of optogenetic tool that can be used in a variety of different situations such as uncaging a protein, in a unique way that allows it to degrade naturally,…show more content…
This event was confirmed by the decreased presence of red fluorescence, which represents the amount of caged HCVp, in membranes after the photocleavage2. I believe this technique is suitable as another study has done an uncaging experiment via the light activated dissociation of a photochromic FP called Dronpa4. PhoCl provides an alternate way to uncage a protein which is not limited by great amounts of activity in the off state2.
Supplementary Figures 5(a),(b),(c) depict the use of mass spectrometry for additional evidence of spontaneous dissociation of PhoCl using mass spectrometry2. It was found that by 3100s the cleavage was at 85%2. I sense that this is a valid technique to determine if a molecule has been cleaved as it mirrors another study which mass spectrometry was used to determine dissociation of a photocleavable
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