Biology
Aim:
My objective is to plan and conduct an experiment from which I should be able to draw a firm conclusion that will either prove or disprove any predictions I make. This essay aims to assess and investigate the effect of various solution concentrations on the activity of osmosis in plant tissue.
Background scientific theory:
Plants exchange gases (CO2 and O2) in maintaining vital respiratory processes and in carrying out photosynthesis; they absorb certain minerals and sugars so to use as a source of energy and eradicate wastes in order to maintain specific requirements for survival. Large amounts of water are absorbed by
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The maximum water potential is nil; an example of which is within pure water hence water molecules will flow from it to any other aqueous solution regardless of how dilute it may be.
There are three possible concentrations of solution. The first, a hypertonic solution, has a higher solute concentration than that of the cell and as a result the water will leave the cell resulting in collapsed vacuoles in plant cells. A hypotonic solution, however, has a lower solute concentration than the solute concentration inside the cell and hence the net movement of water inside the cell is at its maximum. Whilst an isotonic solution is when the concentration of solutes is the same inside the cell as it is outside of the cell; in such as case the cell would not lose nor gain any water. This state is otherwise recognized as dynamic Equilibrium where the molecules are randomly distributed despite there still being a rapid net movement of water across the cell membrane in both directions; water movement in opposing directions occur at the same rate and thereby ‘cancel’ each other out.
Larger differences between the concentrations or water potential of the solutions results in a steeper concentration gradient, meaning a faster rate of osmosis. In effect the concentration outside of any cell is proportional to
When the solutions are isotonic, there is no net flow of water across the membrane.
As the lab introduction explains, osmosis is relatively permeable to water and will follow solutes. By instinct, the water will move from a more diluted solution to more of a concentrated solution. The products of the experiments concluded the physiological significance of osmosis by how cell membranes in the body are semipermeable meaning that only certain molecules can pass through it. When intracellular fluid and extracellular fluid are at equilibrium by non-penetrating and concentrated solutes, no net movement of water goes in and out of the cell. Furthermore, if the ECF changes in osmolality, then depending on the difference between the ECF and ICF will determine whether water moves in or out of the cell. This is important in the cell membrane as small differences in osmolarity correspond to large, rapid change in osmotic pressure, causing cells to gain or lose water. In sum, our body makes critical decisions in what molecules are allowed to penetrate the cell membrane and make sure that our red blood cells don’t cause any problems within the
Osmosis is one of the most common and important processes in biology. Countless processes within the human the human body rely on osmosis and other forms of diffusion, ranging from the exhale of CO2 out of the cell to the various reactions between organelles (Nave, n/d). Osmosis itself is the diffusion of water across the cellular membrane in and out of a cell. The direction of net movement is decided by the concentration of water and solutes on either side of the cell, always moving towards the side with the lesser concentration of water (Nave, n/d). The concentration is solutes is called the Tonicity, and this dictates the movement of osmosis across a membrane (Kahn Academy, n/d). To test this relation, potato cores were placed in different solutions with varying levels of sucrose and water. The common conception was that the increase in sucrose within a solution will cause osmosis to occur to a lesser extent between the potato and the solution. To measure this, the potatoes were weighted after a night of soaking in the solutions to test for a change in weight. It was the general conception that the greater the amount of sucrose within the solution, the lighter the potato will be.
Osmosis is described in one of three ways when comparing more than one solution. The cell’s external and internal environment helps determine tonicity, which is defined as how the cell reacts to its environment. When the cell’s environment is equal in osmolarity to itself and there is no change, it is considered an isotonic solution. When the environment has a higher osmolarity, shrinkage occurs and it is considered a hypertonic solution. When the environment has a lower osmolarity, swellings occurs and it is considered hypotonic.
In osmosis, the flow of the water from or to a cell depends on whether the cell is immersed in a solution that is isotonic, hypotonic, or hypertonic to the solution. If the cell is isotonic to a solution, this means that the solute concentration of a cell and its environment is the same and therefore there will be no movement of water. If the solute concentration is lower than that of the cell, then water will flow into the cell, causing it to expand. If the solute concentration is lower than that of the cell then water will flow out of the cell, causing it to shrink.
Osmosis is the movement of water molecules from high concentration to low concentration through semipermeable membranes, caused by the difference in concentrations on the two sides of a membrane (Rbowen, L.). It occurs in both animals and plants cells. In human bodies, the process of osmosis is primarily found in the kidneys, in the glomerulus. In plants, osmosis is carried out everywhere within the cells of the plant (World Book, 1997). This can be shown by an experiment with potato and glucose/salt solution. The experiment requires putting a piece (or more) of potatoes into glucose or salt solution to see the result of osmosis (a hypertonic type of solution is mostly used as it would give the most prominent visual prove of
Within every cell, a movement of a solvent occurs through a semipermeable membrane to equalize the concentration of solute on both sides of the membrane. The diffusion of water across the cell’s membrane down to its concentration gradient is called osmosis. In this case, the concentration gradient is the difference of density between one side of the cell membrane to the other. Since the cell’s membrane is permeable, particles can flow freely in and out of the cell, but the net flow will be strong in the direction of lower concentration until the system has reached a stage of equilibrium, the point at which both sides of the membrane are equal. In the
Osmosis is the passive movement of water from an area of low solute concentration to an area of high solute concentration, normally across a membrane which prevents the movement of solvent. This is a process by which materials may move into, out of, or within cells. Osmosis doesn’t depend on energy provided by living organisms but is affected by the properties of the cell membrane. The rate of osmosis is dependent on such factors as temperature, pressure, molecular properties such as size and mass, and the concentration gradient. In osmosis, the relationship between a solute’s concentration outside of cell and inside of a cell is described in terms of the tonicity of the solution outside of the cell. A cell is in a hypotonic solution when the solute is more concentrated inside the cell and therefore water moves into the cell. In this solution the cell swells as water enters, this may continue until it ruptures or hemolyzes. In the reverse condition, the cell is in a hypertonic solution
The movement of water molecules across a semi-permeable membrane is the process of osmosis. If there is a solute and a solvent, each containing different concentration levels, then the water would move along its concentration gradient until each side of the membrane are equal. The water moves because the membrane is impermeable to the solute and the solute concentrations may differ on either side of the membrane. Water molecules may move in and out of the cell, but there is no net diffusion of water. Water will move in one direction or the other, and this is determined by the solute or solvents concentration levels. If the two solutions are of equal concentrations, they will be isotonic. If the concentrations are unequal, the
In this experiment, the osmotic concentration is found with potato slices placed in sucrose solutions. Osmosis in this model is the net movement of water between the potato cell and the sucrose solution. The movement of water is determined by the molarity of sucrose. As the molarity of sucrose increased then the concentration in the solution also increased. H2O will move through the cell membrane to areas of higher concentration in order to reach equilibrium. If cells are placed
This experiment was used to examine the hypothesis that: Osmosis is dependent on the concentrations of the substances involved.
The difference is that along with large molecules, living cells prevent molecules with positive charges and solubility. This is not representing in dialysis tubing, and is only found in living cells because the tubing is only based on molecular size (98). When referring the rate of diffusion, the concentration gradient influences the diffusion rate, based on the factors of temperature. The ability for molecules diffuse from high to low concentrations primarily depends on the concentration gradient between the two areas.(96-99). My hypothesis for the study is that in the hypotonic, hypertonic, and isotonic solutions, the direction and rate of osmosis will determine based on the concentration inside the dialysis tubing. My prediction is that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant.
Finally, it could also be a hypertonic solution which is when there is a higher concentration of solute in the solution than in the cell and therefore the water leaves the cell. This make the cell plasmolyzed or “shrunken”. In our experiment this means that the potato cell would
The purpose of this lab is to test the effect of osmosis on cucumber slices. If a cucumber slice is placed in a hypertonic solution, then the mass of the cucumber slice will decrease. Whereas, if
The aim of the sixteenth of November experiments was to observe how three different solutions with various sucrose concentration influenced osmosis in relation to three onion cells and the impact on the cells structure.