P450 Week 1 Lab Report

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

IC50: 1.5 μM, 3.5 μM and 0.5 μM for Panc02, MCF-7 and T47-D cell lines, respectively

In this case, the encryption password is unique to the Windows account encrypting the file. In this case, it also happens to be “Pa$$word.” Here is a screenshot showing the file post-encryption.

A) Describe in your own words, in as much detail as you can, the anaerobic metabolism of glucose to pyruvate. B) Draw this pathway (by hand), indicating all substrates, enzymes, cofactors and products. (You do not need to include reaction mechanisms.)

Make three exposures using given technical factors on a phantom knee in PA position . Include saline bags in exposures 1 and 2 to demonstrate patient soft tissue thickness.

To finish week 5 we discussed the importance of healthcare professionals being informed about their patients' current CAM use. The importance of CAM is something all healthcare professional need to know and understand because this can be a life threatening issue if a professional does not know about it. In addition as a healthcare professional I must know the back ground of the patient to make sure that the proper care is being given to the patient. It is the duty of the healthcare provider to ask all the proper questions and to get the patient to open up. The power point mention how popular CAM is becoming this is why precautions must be taken, “The widespread use of CAM has produced government agencies who are charged with investigating health

TCO 1: Given a simple problem, design and desk-check a solution that is expressed in terms of pseudocode, flowchart, and/or input-process-output (IPO) diagrams.

The use of selective chemical inhibitors of human cytochrome P450 enzymes is a powerful method by which the relative contributions of different human P450 enzymes to the drug metabolism can be obtained. However, the contribution of CYP2B6 in the metabolism is more challenging due to the lack of a well-established inhibitor.

In order to find the vertex of f(x) = x² -6x +3→ I first need to find the x coordinate value of vertex.

Where π1 and π2 are prior probabilities of good and bad credit populations, Q (2∣1) and Q (1∣2) compute the probability of making Type I errors, i.e., a customer with good credit is misclassified as a customer with bad credit, and Type II errors, i.e., a customer with bad credit is misclassified as a customer with good credit, and A (2∣1) as well as A (1∣2) are the corresponding misclassification costs of Type I and Type II errors.

2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.

Cytochrome P450s (CYPs) are present in all three domains of life and constitute a superfamily of heme-containing monooxygenases. CYPs are involved in the metabolism of endogenous and xenobiotic compounds 1-4. CYPs belong to a variety of families with two global sub-families, CYP51 and CYP61. CYP51 is involved in sterol biosynthesis and is reported as housekeeping CYP in fungi and is an important target for the antifungal drugs 3, 5. CYP51s are found in sterol-producing animals, plants, protists, but rarely in bacteria, producing 14-α-demethylated sterols 6. The CYP51 reaction occurs in three steps, each reaction requires one molecule of oxygen and two NADPH-derived reducing equivalents 7-9. Another cytochrome P450 CYP61 (sterol 22 desaturase) represents an ancient activity, as the superfamily is present as CYP710 in plants 10. Plant P450s are generally classified as strong candidate of the C-22 desturases that produce stigmasterol and

The article “Bacterial snack attack deactivates a drug” by Christian Jobin discusses how the presence of certain bacteria among cancer cells can pose complications with chemotherapy. Jobin begins the article by giving a brief explanation of the importance of bacteria naturally found in the human environment. Jobin continues to explain the significance of these bacteria writing “our bacterial population contains millions of genes encoding enzymes that can process substances that have been derived from nutrients or the environment, or that have been administered as drugs.” Therefore, bacteria play a very crucial role in maintaining normal body functions.

To fully understand the biological role of isoketals in oxidative injury and counter their detrimental effects, efforts to identify selective scavengers of isoketals were undertaken. A lead compound, pyridoxamine (PM) was first discovered as a carbonyl scavenger in 1996 by Billy G. Hudson and colleages (Booth, Khalifah et al. 1996), and identified through initial screens, where Amarnath and colleagues determined second-order reaction rates for a series of primary amines relative to N-α-acetyl-lysine (Amarnath, Amarnath et al. 2004). Pyridoxamine is a vitamer in the vitamin B6 family, and through our studies, PM was found to effectively intercept isoketals from adducting to cellular amines. Through the initial screen, pyridoxamine was found

The impact of certain estrogenic xenobiotics on the reproductive system development and health of animals has been clearly documented. Findings, such as ours demonstrate thathumans are also exposed at risk. As data accumulate regarding to infertility, genital tract malformations and increasing cancer rates in estrogen target tissues (especially the breast),