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Photosynthetic And The Function Of Eukaryotic Organelles That Are Crucial For The Survival Of All Life On This Planet

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Practical 6: Photosynthetic responses to light regimes
Photosynthetic eukaryotes use chloroplast to convert light energy from sun into chemical energy (ATP to NADPH). This is then stored in sugars (happens in dark reactions) and other fuels. This reaction underpins all life on earth. Light energy travelling from the sun has visible lights which consist of red, orange, yellow, green, blue, violet and indigo while invisible lights are ultraviolet and infrared light. In this study spinach leaves consisting living chloroplast will be isolated to investigate photosynthesis. This will be used to analyse the structure and the function of eukaryotic organelles that are crucial for the survival of all life on this planet-chloroplasts (Lilley et …show more content…

This would have major impact on plant growth and floor (Battaglia et al. 2003; Endler 1993). This is connected with the wavelength of the light and much of this research is based on observation of the highest amount of photosynthetic activity, that is measured with samples that are exposed to dark (unfiltered) light at different wavelengths (Lee 1987). Therefore the aim of this experiment is to assess the photosynthetic activity using chloroplast isolate from spinach beet. This study will determine how wavelength affects the rate of photosynthesis. Based on past studies and research it can be hypothesised that chloroplast exposed to white light would have quicker photosynthetic activity then those exposed to red light. This is expected to occur because chlorophyll a and b consists of accessory pigments which inhibit different wavelengths of light, as a result this would raise the amount of light energy being consumed. The control of this study will be light independent reaction as chloroplasts are kept in dark to stop photosynthesizing due to inadequate development of ATP and NADPH from light reaction. While the reaction in room is hypothesised to photosynthesis at a lowest possible rate (Sadava et al. 2011)
Method: Chloroplast isolation from spinach
Collected ~4 grams of provided spinach leaf and teared it into pieces, removing

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