Practical 6: Photosynthetic responses to light regimes
Photosynthetic eukaryotes use chloroplast to convert light energy from sun into chemical energy (ATP to NADPH). This is then stored in sugars (happens in dark reactions) and other fuels. This reaction underpins all life on earth. Light energy travelling from the sun has visible lights which consist of red, orange, yellow, green, blue, violet and indigo while invisible lights are ultraviolet and infrared light. In this study spinach leaves consisting living chloroplast will be isolated to investigate photosynthesis. This will be used to analyse the structure and the function of eukaryotic organelles that are crucial for the survival of all life on this planet-chloroplasts (Lilley et
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This would have major impact on plant growth and floor (Battaglia et al. 2003; Endler 1993). This is connected with the wavelength of the light and much of this research is based on observation of the highest amount of photosynthetic activity, that is measured with samples that are exposed to dark (unfiltered) light at different wavelengths (Lee 1987). Therefore the aim of this experiment is to assess the photosynthetic activity using chloroplast isolate from spinach beet. This study will determine how wavelength affects the rate of photosynthesis. Based on past studies and research it can be hypothesised that chloroplast exposed to white light would have quicker photosynthetic activity then those exposed to red light. This is expected to occur because chlorophyll a and b consists of accessory pigments which inhibit different wavelengths of light, as a result this would raise the amount of light energy being consumed. The control of this study will be light independent reaction as chloroplasts are kept in dark to stop photosynthesizing due to inadequate development of ATP and NADPH from light reaction. While the reaction in room is hypothesised to photosynthesis at a lowest possible rate (Sadava et al. 2011)
Method: Chloroplast isolation from spinach
Collected ~4 grams of provided spinach leaf and teared it into pieces, removing
Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.
There are two main types of chlorophyll, chlorophyll a which absorbs wavelengths of 430nm (blue) and 662 (red) and is the main photosynthetic pigment, and chlorophyll b, which doesn’t directly participate in the photosynthetic process, but is capable of donating its energy to chlorophyll a
Introduction: Photosynthesis can be defined as a solar powered process that removes atmospheric carbon dioxide and transforms it into oxygen and carbohydrates (Harris-Haller 2014). Photosynthesis can be considered to be the most important biochemical process on Earth because it helps plants to grow its roots, leaves, and fruits, and plants serve as autotrophs which are crucial to the food chain on earth. Several factors determine the process of photosynthesis. Light is one these factors and is the main subject of this experiment. The intensity of light is a property of light that is important for photosynthesis to occur. Brighter light causes more light to touch the surface of the plant which increases the rate of photosynthesis (Speer 1997). This is why there is a tendency of higher rates of photosynthesis in climates with a lot of sunlight than areas that primarily do not get as much sunlight. Light wavelength is also a property of
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
The purpose of this experiment was to investigate the effects of light intensity on the rate of photosynthesis in a Moneywort plant. By observing the plant in distilled water mixed with sodium bicarbonate, different light bulbs were targeted onto the plant. The measurement of the amount of bubbles present on the plant during the trial of the experiment enabled us to identify the comparisons between the activity of the light and the process of photosynthesis.
The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the
Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen and nitrogen and are bound more tightly to the paper than the other pigments.
The initial experiment was a success. As our treatment group spent more and more time under the lights, the absorbance rate continues to decrease toward zero. Once our 30 minutes were up, the absorbance rate in each tube was significantly lower than at the start of our experiment. In contrast the two control groups did significantly lower the absorbance. Each control lacked one of the vital aspects of photosynthesis, one being light, and the other being chloroplast. Neither of the control groups (Control 1 or 2) showed any signs of photosynthesis. Control 1 was exposed to light, but contained no photosynthetic organelles thus the absorbance throughout the 30 minutes varied minimally, mostly staying stagnant. Control two which contained chloroplast but was not exposed to any light failed to lower the absorbance at all and in fact increased the absorbance over the 30 minutes. However, the treatment group contained both and ultimately performed photosynthesis as we expect therefore, confirming our assumption that chloroplast were the organelles required for photosynthesis in plants and that light is required to perform said photosynthesis. The treatment group, containing both the chloroplast and being exposed to light provided evidence that photosynthesis was taking place as the absorbance lowered at each 10-minute interval. Having a less absorbance would be desired because as DCIP became reduced we would expect the solution to become more and more clear, thus less
This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.
The chloroplasts are organelles found in plant cells. The chloroplast is the site of photosynthesis. Since chloroplast is the site of photosynthesis it is also where sugar is produced, because glucose is a product of photosynthesis. To begin with, I made a solar power sugarcane processing plant. The reason being is that the chloroplast is where photosynthesis takes place and a product of photosynthesis is glucose (sugar), and a sugarcane processing plant produces sugar. In photosynthesis, light energy from the sun is converted into chemical energy, which is then stored and also used to make glucose. I have a light in the corner to represent the sun and solar panels on the roof, which shows energy transformation.
For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.
However, the photosynthetic process can be affected by different environmental factors. In the following experiment, we tested the effects that the light intensity, light wavelength and pigment had on photosynthesis. The action spectrum of photosynthesis shows which wavelength of light is the most effective using only one line. The absorption spectrum plots how much light is absorbed at different wavelengths by one or more different pigment types. Organisms have different optimal functional ranges, so it is for our benefit to discover the conditions that this process works best. If the environmental conditions of light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will increase with average light intensity and under the wavelengths of white light which will correspond to the absorption spectrum of the pigments. The null hypothesis to this would be; if the environmental conditions light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will decrease with average light intensity and under the white light which will correspond to the absorption spectrum of the pigments.
Because chlorophyll-a takes in violet-blue, and orange-red lights to use in photosynthesis, it reflects green, and that is the color we see. This study investigates the relationship between the wavelength of light and the total respiration of the plant Elodea. The purpose is to see the effect of the presence or absence of certain wavelengths can have on the process of photosynthesis. Because Elodea is a plant that reflects green light, it can be assumed that red lights, the complimentary color to green will be absorbed the most contributing to the process of photosynthesis and respiration making the most oxygen.
Without photosynthesis we would not be able to receive energy. We should be more appreciate of plants, without them we would not survive. This paper will explain the basic components require for photosynthesis, the role of chlorophyll, how energy is transferred, and photosystems I and II and the most precious product results of photosynthesis.
Chlorophyll is a molecule that is found in plants to help them photosynthesize. Photosynthesis is when plants take sunlight and convert it to food and energy. Plants have chlorophyll in them that help them photosynthesize and help make plants appear green. Chlorophyll is the part of the plant that absorbs and reflects the colors in UV rays to make the plants appear green. This is why if you ever look at a plant through a microscope you will see little green circles, these are the chlorophyll.