First: Soaking wet gel excessive amount of ethanol at room temperature with a change of ethanol per day for three days and then replaced by ethanol by Regular hexane, which has the surface tension less where soak gel excessive amount of regular hexane at room temperature and the new regimes hexane day for three days in order to make sure fully replace solvent, then drying the gel in different ways as
Fig. 12 CXL10-/- mice are relatively protected against FFC-induced liver injury and inflammation. WT & CXCL10-/- mice were fed either chow or FFC-diet for 20 weeks. (A) Plasma alanine aminotransferase (ALT) levels were measured. (C) Total RNA was extracted from liver tissue and mRNA expression of surface macrophage marker cluster of differentiation (CD)68 was evaluated by real-time qPCR. (D) Assessment of macrophage infiltration in fixed liver tissue was done by immunohistochemistry using macrophage galactose-specific lectin (Mac-2) antibody. Bar columns represent mean ± S.E.M. *** P < .001, * P < .05 compared to WT chow-fed mice.
The experimental procedures for Lab 2 were provided on Blackboard labelled as “Pre-Lab 2: Techniques & Measurement”.
In this experiment, the relative rates of free-radical chain bromination where determined. Five arenes were used for this comparison along with two controls for each set. One set was kept in the dark while the other was put under light. This allowed for better observation of the reactions, as the light set would proceed fast to show which arenes reacted slowest, while the dark set would proceed slowly to show relative differences between the faster reacting arenes. The time it took for the arenes to react was recorded to determine the relative rates of the reactions.
With an auto-pipet, 400 l of cyclohexanone was placed into a large sample vial. 1000 l of methanol was added with the cyclohexanone. The sample vial was then capped and the solution was swirled gently. In the hood, 1200 l of sodium borohydride reducing solution was added to the solution by adding it in dropwise. The solution was then swirled and vented occasionally for 25 minutes. After letting it sit and swirling the solution, 4.0 ml of cold dilute hydrochloric acid (1 M HCl) was added into the mixture using a calibrated pipet. The aqueous mixture was extracted with the use of three 2.0 ml portions of methylene chloride. With each addition, the mixture was capped, shook gently, ventilated, and given time for the layers to separate. 2013 mg
Add 310 μl buffer AVE to the tube containing 310 μg-lyophilized carrier RNA to obtain solution of 1 μg/μl. Dissolve the carrier RNA thoroughly, divide it into conveniently sized aliquots, and store it at –20°C. Do not freeze–thaw the aliquots of carrier RNA more than 3 times.
Purpose: is to determine the unknown bacteria with a variety of biochemical tests. There are many reasons that contribute to why it is so important to test patients for both high and low risks diseases. The most important reason would be to know the identity of microorganism and how it can be treated .This study was performed in microbiology laboratory class by applying the microorganism to the tests that have been performed in the class prior to the identification of the unknown. First, the lab professor handed out a bacteria that was on the unknown streak plate labeled B5 that consisted of an unknown gram positive or gram negative bacteria.
Hepatitis C virus deadly virus which needs and demand permanent cure to serve the people affected across the globe. Molecular designing studies, led to the identification and development of Quinoxaline based new chemical entities, their putative binding site, key interactions within NS3h protein of HCV, and druglike properties of designed molecules are discussed. The condensation of 3- hydroxynopinone with chiral diamines such as
This experiment consisted of a simple distillation of a hexane and heptane mixture. The first, third, and fourth fraction were taken and recorded in Table-1. The first fraction temperature ranged from 29.7°C – 38.2°C. The second fraction temperature range was recorded as 40.3°C-41.7°C, but this fraction was not used. The third fraction temperature range was determined to be 41.8°C – 36.5°C. Finally, the fourth fraction temperature range was 34.5°C-27.3°C. The ranges of the temps following the second fraction began to decrease, causing a discrepancy in the data. This decrease in data can be linked to the formation of water or condensation on the tip of thermocouple. The first, third, and fourth fractions were collected and further analyzed using
Poly (3-hexylthiophene) Molecular Bottlebrushes via Romp Macromolecular Architecture Enhanced Aggregation is a process, which uses the Grignard metathesis polymerization. Grignard metathesis polymerization is a technique that uses a living chain growth mechanism in order to produce the regioregular polymer poly (3-hexylthiophene). The synthesis demonstrates the addition of poly (norbornene) backbone and rr-P3HT side chains. Grignard metathesis is conducted in order to allow the polymerization of poly (3-hexylthiophene) to take place in a room temperature and to decrease the cost and increase the efficiency of the technique. Poly (3-hexylthiphene) the product of head to tail coupling is a class of polymers that maintain a great level of solubility,
The scientist investigated the compound 1,3,5-trisilacyclohexane which they assume to exist in chair conformer like cyclohexane. The Silicon atom in the compound has a lower in electronegative and much larger in radius compared to the Carbon atom of cyclohexane making the structure more flexible. The property of silicon atom in the compound also makes the C-Si bond larger and the C-Si-C bond smaller making the structure
The baseline is a common term for most chemical reactions. However, in this experiment, it is used to authorize a basic for a reaction. With the dependent variables being the substrate or the enzyme in this experiment, you have to point out and understand what is happening in the reaction. The baseline may vary with different situations that are applicable to the design of this experiment. In this experiment, the effects of changing a coincidental variable may possibly be resolved.
calculated molar mass for both dish #1 and #2 is 148 g/mole. On comparing both the molar
4.65.. m/ 1.145 m = 4.06.. 4 HVL are required to stop the 94% beam.
The goal of this experiment was for students to create 6-8 labs to discern among four different clear substances, which is water. Students tested and observed for evaporation, smell/observation, viscosity, surface tension, density, and created two of their own to compare to the control in the experiment. Each lab was created to observe the properties of water. It was imminent that water is a solvent due to the tea grains, has capillary action due to its ability to rise into the cotton ball, evaporates when in the presence of heat, and is far more dense than oil.
In chlorambucil treated rats for 5 days (Group III), congested dilated central veins and portal tract were observed. Vacuolated hepatocytes were also detected. Most hepatocytes were noticed with dense pyknotic nuclei . (Figs. 3,4 ). In chlorambucil treated rats for 10 days (Group IV), hepatocytes with cytoplasmic vacuolations and congested central veins were frequently noticed. Hepatocytes with abnormal pyknotic nuclei were also detected. (Figs. 5,6 ) While in chlorambucil treated rats for 15 days (Group V), extensive vacuolated hepatocytes with pyknotic nuclei were seen in most hepatic lobules. Dilated congested central veins, hepatic sinusoids and portal tracts were also detected. Areas of mononuclear cellular infiltration were also