Taking a Look at Electrophoretic Deposition

Fig1 shows on the right group one next to it, 1st half of our result on electrophroresis gel. The 2nd half for the gel is our experiment compared to Anderson group 6. The 1st layer in all 3 pictures is the bound dye, the 2nd layer is the free dye.

#1) What is an electrochemical gradient, and why is it so important when discussing the movement of ions across cell membranes?

The chemical reaction which occurs between the electrolyte and the positive electrode internally in the battery then produces a buildup of positive ions, which are atoms that are missing electrons. The positive ions buildup at the cathode of the battery which

In the diffusion experiment, we sought to understand how diffusion works across a plasma membrane by simulating a caterpillar consuming plants. My hypothesis was: If we test the diffusion using Lugol's solution, we will find the dialysis tube to be semipermeable. We used three beakers: one small beaker, and two larger beakers, as well as dialysis tubes, clamps, two pipettes, starch, Lugol, test strips (to test the glucose level), and amylase.

In slab gel electrophoresis agarose or polyacrylamide is used. The conducting buffer is contained in the porous gel and the gel is then poured in between glass plates. These plates are separated by spacers. An electric field is applied at the rear after the substance of experimentation is added to the wells at the top. The substance will then move a certain distance towards the positive electrode. This method uses gel unlike the cellulose acetate strips but it still uses a buffer and a electric field to migrate the substances based on their size and charge. Slab gel electrophoresis is usually used in the biological

Michael Gugliotta 12/3/14 Chemistry Period 8 Electrolysis Lab During this experiment, we saw electrolysis in action. Electrolysis occurs once an electrical wave hits an object. Electrolysis only occurs when it is in a salt-based or an ionic compound.

Ceramic on UHMWPE is one of the most commonly used alloys in biomedical implant materials. This alloy is used for artificial

The purpose of the DNA restriction and electrophoresis lab was to first become familiar with the properties of restriction enzymes and discover that they, along with agarose gel electrophoresis, are used to characterize DNA molecules. Restriction enzymes are used to make cuts or join together DNA fragments. The cuts result in either staggered cleavage, or blunt cleavage. Staggered cleavage results when the breaks are offset. This cleavage usually results in sticky end being produced because for each DNA end that is produced there are extensions with unpaired nucleotide bases. Blunt cleavage results when the breaks in the phosphodiester bonds are right across from each other.

Modern medicines, like insulin, are made by using genetically engineered bacteria. Bacterial transformation is used to engineer bacteria to produce these medicines. Scientists are able to transfer DNA from other organisms to bacteria, commonly through plasmids, to transform the bacterial DNA. Scientists have been able to artificially stimulate the bacteria to uptake certain chosen genes and then incorporate them into their genomes through transformation. This is often done by the use of a heat shock process in order for the chosen genes to be transformed into the bacteria’s original DNA. If the transformation process was successful, the foreign genes in the transgenic bacterium will be expressed through the production of proteins. The bacterium have the ability to clone themselves and a fast

The primary productivity of Elodea in response to different wavelengths of light. The term production initially means the creation or making of something, for instance organic matter. However productivity is the rate of growth or biomass increase in plants (or animals). This is increase of biomass or growth in plants, is generally caused by photosynthesis (6 CO2 + 6 H2O Light Energy C6 H12 O6 + 6 O2), which converts light energy into chemical energy therefore creating new plant tissues and new compounds and structures are synthesised and cells division. In order for photosynthesis to occur, a plant requires sunlight, carbon dioxide and nutrients.

The Fe-Co deposit obtained from ethaline and reline was successfully deposited on a copper substrate by maintaining a steady potential of 3.0 V at 60℃ for 2 hours. The Fe-Co coating from reline was dark black in colour, whereas the Fe-Co coating from ethaline appeared to show a tint of grey on the surface. The Fe-Co deposits in both liquids were smooth and uniform. Figure 6.3 a and b show the SEM images of Fe-Co deposited from ethaline and reline. It is clear from the images that there is a variation in morphology from both liquids. The Fe-Co coating from reline appears to show a more nodular shaped structure which is evenly spread on the surface of the copper substrate when compared to Figure 6.3 a.

Liquid chromatography would be best because of the differing polarities of illicit drugs. A stationary phase will be chosen that has a polarity that will bind to each of the different drugs in slightly different affinity

Take an electrophoresis chamber and take out the UVT gel tray within the chamber. Grab a millimeter grid and place it on the electrophoresis chamber stage, then place the UVT gel tray on top of the millimeter grid.

Diffusion Lab James D. Motley Biology Lab B Period 2 Uma V. CECP ABSTRACT: In this lab I used water, agar cubes, a beaker and a scale to see whether diffusion increased or decreased the volume of the agar cubes. I let the cubes sit in water overnight, then weighed them to see if the volume changed. In conclusion, the volume increased, because of the water soaking into the cubes.

In order to test the hypothesis that the WRKY75 transcription factor directly regulates FLS2 expression, electrophoretic mobility shift assay (EMSA), the CRISPR/Cas9 and microarray techniques can be implemented. Firstly, an EMSA will be conducted to determine whether WKRY75 is a transcriptional factor that regulates and binds to pFLS2. The DNA probe that will be used is the pFLS2 double-stranded DNA sequence, which contains the regulatory element. WRKY75 will be determined as a binding protein if we observe band shift, as electrophoresis separates molecules based on charge and size; therefore, DNA attached to a protein will take longer to migrate through the gel. If WRKY75 is determined to be a transcriptional factor of pFLS2, we can implement the CRISPR/Cas9 technique.