Roben Houspian 2/27/15 Muscle Physiology 1
Abstract: In this experiment the measurements of skeletal muscle fibers of the rabbit are in millimeters. The average length for the three muscle fibers after adding the solution A which contained only 0.25% ATP in distilled water was 20 mm. The average length for the three muscle fibers after adding the solution C which contained 0.5M KCl and 0.001M MgCl2 in distilled water was 1.77 mm and the average length for the three muscle fibers after adding the solution B which contained 0.25 % ATP and 0.5 M KCl with 0.001 M Mgcl2 in water was 1.77 mm.
Introduction: According to the “Human Physiology Laboratory Manual “,BIOL 282 ,page 31 , the reason of performing this experiment is to learn how the muscle contraction occurs based on the molecular level and what kind of factors are involved .As a matter of fact, skeletal muscles contain a lot of nuclei because of the cell fusion while being developed and are made of cylindrical cells that have myofibrils. The myofibrils contain sarcomeres and the
Muscle fibres, as shown in Diagram 1, consist of myofibrils, which contain the proteins, actin and myosin, in specific arrangements . The diagram illustrates how a muscle is made up of many fascicles, which in turn are made up of many endomysiums, and within them, many muscle fibres. Each muscle fibre is made up of many myofibrils that consist of sarcomeres bound end on end . Actin is a thin filament, about 7nm in diameter, and myosin is a thick filament, about 15nm in diameter , both of which reside in the sarcomere. They are held together by transverse bands known as Z lines . Diagram 2 shows actin and myosin filaments within a sarcomere, and the Z lines that connect them.
Martini, F. H., Nath, J. L., and Bartholomew, E. F. “Muscle Tissue.” Anatomy & Physiology. 9th
The purpose of this lab was to better understand the human body by doing various activities and observing the three different muscle types. The activities were not conducted in order, but we did follow the instructions of each activity. However, there could be deviation since Vivian had allergies and I was sort of sick as well so our result may not be as accurate. By conduction the activities, we were able to analyze the different receptors in the skin and sense—for instance understanding the differences in receptors or taste and smell. Also, we were able to differentiate the three muscle types and observe the striation of the cardiac and skeletal muscle—and observe other differences in muscle types. By noticing that, for instance the tongue has different regions for different taste, we were able to infer that some taste are more important to distinguish than others. Also, by observing the different types of muscle, we were able to discuss the different functions and why the cardiac and skeletal muscle have striation. Lastly, it is important to understand the human body, not
Myofibrils are made up of long proteins that include myosin, titin, and actin while other proteins bind them together. These proteins are arranged into thin and thick filaments that are repetitive along the myofibril in sectors known as sarcomeres. The sliding of actin and myosin filaments along each other is when the muscle is contracting. Dark A-bands and light I-bands reappear along myofibrils. The alignment of myofibrils causes an appearance of the cell to look banded or striated. A myofibril is made up of lots of sarcomeres. As the sarcomeres contract individually the muscle cells and myofibrils shorten in length. The longitudinal section of skeletal muscle exhibits a unique pattern of alternating light and dark bands. The dark staining, A-bands possess a pale region in the middle called the H-zone. In the middle of the H-zone the M-line is found, that displays filamentous structures that can join the thick filaments. The light-staining bands also known as I-bands are divided by thin Z-line. These striated patterns appear because of the presence of myofibrils in the sarcoplasm (IUPUI, 2016).
Actin and myosin filaments can be found in skeletal muscle and are the smallest units that form a sarcomere, which is the smallest contractile unit in muscle (Baechle, 2008). The Sliding Filament Theory states that the actin filaments slide inward on the myosin filaments, pulling on the boundaries of the sarcomere, causing it to shorten the muscle fiber, also known as a concentric muscular contraction (Baechle, 2008). The Sliding Filament Theory is composed of five steps: the “Resting Phase”, the “Excitation-Contraction Coupling Phase”, the “Contraction Phase”, the “Recharge Phase”, and the “Relaxation Phase” (Baechle, 2008). During the Resting Phase, the actin and myosin filaments are lined up with no cross-bridge binding of the two filaments. During the Excitation-Contraction Coupling Phase, Calcium is released from the sarcoplasmic reticulum and binds to troponin, causing a shift in tropomyosin where the binding cites are exposed (Baechle, 2008). When the binding cites are exposed, the myosin cross-bridge head attaches to actin. During the Contraction Phase, ATP bonds break, releasing energy that is used to allow the myosin head to flex, causing the actin filaments to move toward the M-bridge. During the Recharge Phase, there is a continuous repetition of the Excitation-Contraction Coupling Phase and the Contraction Phase in order to produce muscular
Of all the tissues in the body, skeletal muscle can adapt the easiest. —- On the outside of the muscle just deep to the sarcolemma, are small cells that lack cytoplasm and help with the reparation of muscles, called satellite cells. During an intense workout, the fibers within the muscle tissue become damaged as they break down and tear. The job of
Muscle is formed with a long and thin tissue called muscle tissue which moves the organs and organisms, and the muscle tissue is made out of a group of cells called the muscle fibers. There are three types of muscle: skeletal muscle, smooth muscle, and cardiac muscle. Skeletal muscle is the muscle who moves the bones, it has much longer fibers than the smooth muscles. Smooth muscle forms the walls of organs, for example, the wall of a stomach. Cardiac muscle is the muscle that forms the heart. From the cross-section view of a muscle, it shows that there is a layer of muscle sheath around the outermost layer of the muscle. The blood vessels that brings glucose and oxygen to the muscle are weaved in the fibers, and there is a layer of epithelial cells around the muscle to keep the fibers together.Muscle tissue can repair itself, but with round scar tissue instead of long, stretchy fibers. Skeletal
Undoubtedly, this article is pertinent to science because it contributes to the development of muscles in
2006). Essentially, the head of the myosin bends inward towards the centre of the sarcomere and pulls the actin until the cross bridge breaks – this process repeats over and over and causes contraction of muscles. However, this entire process can only occur until specific conditions. Calcium must be present in order to cause troponin to reposition tropomyosin to expose the myosin binding sites and cause the cross bridge cycle to occur; if calcium is absent, troponin cannot reposition tropomyosin to open myosin binding sites (Hopkins. M, P. 2006). The sarcomere is what provides force in a muscle through its myofilaments. In this experiment, the sarcomere is being stretched involuntarily and the power stroke is being observed. When the action potential runs down the axon and calcium is released into the cytosol, the peak contractile force can now
Introduction: Skeletal muscle contraction happens when Ca2+ floods into the muscle cell binding with troponin allowing actin and myosin to bind. The actin and myosin cross bridges bind and contract using ATP as energy. Muscle fatigue is defined as the inability to maintain a desired power output. Muscle contraction can be affected by peripheral fatigue which refers to fatigue mediated by factors outside of the central nervous system, specifically within the muscle fibers, or central fatigue which involves the central nervous system and occurs predominantly due to afferent reflexes that inhibit output from the motor cortex. Peripheral fatigue can be caused by decreased levels of any of the molecules/metabolites involved in muscle contraction.
Muscle analysis. The amount of astaxanthin in muscle in trout fed with a low fish oil diet was 6.3 ± 1.6 g per gram of muscle and in the case of the diet with a higher level this concentration was 9.7 ± 2.2 g per gram of muscle. Therefore, rising the proportion of fish oil in diet, a 54% increase in pigmentation can be obtained. A higher amount would be expected with a longer period of administration and further experiments will clarify this assertion. As our previous work has shown {Salvador, 2007 #7} carotenoid deposition in muscle was affected by the diet. In that work, trout were fed three different diets and muscle canthaxanthin deposition was higher when phospholipids were associated with fish oil in the diet.
The contractile unit of a muscle cell is the sarcomere. Sarcomeres are mostly comprised of actin and myosin which pull and slide upon each other. These contractile units are linked end to end, like a chain, throughout the length of any given muscle. Certain proteins link the ends of these chains to the cell membrane. When a normally healthy individual exercises, some of these fibers, both in the sarcomere and at the connections to the cell wall, will be broken down due to damage (Leyva, 2013). Associated with this process includes the rebuilding of these fibers, in which the body builds back what was damaged stronger than before the damage occurred (Leyva, 2013). One of these end proteins is dystrophin. The purpose of this paper is to explore the implications of insufficient production of dystrophin, as in DMD.
skeletal and cardiac muscles thus supporting the idea that it is linked to muscle organization. The
Skeletal muscle plays a pivotal role in regulating systemic glucose homeostasis in part through the conserved cellular energy sensor AMP-activated protein kinase (AMPK). AMPK activation increases glucose uptake, lipid oxidation and mitochondrial biogenesis, thereby enhancing muscle insulin sensitivity and whole-body energy metabolism. Here we show that the H19 long noncoding RNA (lncRNA) post-transcriptionally increases expression of the atypical dual-specificity phosphatase 27 (DUSP27) which forms complexes with and activates AMPK in muscle. Consistent with decreased H19 expression in muscle of insulin resistant human subjects and rodents, mice with genetic H19 ablation exhibit musclesystemic insulin resistance and altered whole-body
Both of these muscles expand and contract as they have complex structures so it is essential how they do this. The cardiac muscle needs the contractions to occur in order to pump blood out of the atria and into the ventricles and round the circulatory system so the structure of this muscle shows the systole of the heart. The contractions of the skeletal muscle also depend on its structure. The binding and releasing of two strands of sarcomere is how the repeated pattern of contractions occurs. ATP is used to prepare myosin for binding to allow the contractions to happen. The skeletal and cardiac muscle also both has elasticity. The elasticity is used to restore the muscles back to their original lengths which enable them to resume back to their original length once they have contracted and been stretched.