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The Use Of Artificial Light Sources And Prevent Any Natural Lighting From Entering The Laboratory

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The majority of laboratories housing Xenopus laevis make use of artificial light sources and prevent any natural lighting from entering the laboratory. Although Xenopus laevis thrive and reproduce over a wide range of photoperiod, recommendations are periods of 14-hour light and 10-hour dark which mimic conditions in a natural habitat (Major & Wassersug, 1998). Inappropriate photoperiods, light deprivation or continuous light may prove detrimental and cause symptoms of lethargy, sterility and even death (Hayes et al., 1998). Slow and steady dimming and illuminating of lights is preferred to abruptly switching on and off, as this allows the animal to adapt to the change and is potentially less stressful to the animal (Reed, 2005).

2.3.6.2 Humidity

Xenopus laevis frogs are completely aquatic. Therefore, controlling humidity levels in an aquarium housing the tanks for these animals is not as crucial as for a species that spends prolonged periods of time on dry land (Reed, 2005).

2.3.6.3 Water provision

Quantity and depth of water provisions are poorly outlined, but as long as the animal can swim around freely, lie fully submerged underwater, avoid contact with other animals or tank walls if desired and swim in any direction without obstruction or hindrance from other animals or tank walls, it is considered acceptable. The quality of water is more important, with a pH maintained between 6.5 and 8.5. (Reed, 2005). Deviations from this, too acidic or too alkaline, can result

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