Transformation Of Recombinant Egfp / Coli And Analysis With Biotechnology And Bioinformatics Tools

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Kelley Matthew

Transformation of Recombinant EGFP/pET41a(+) Plasmid DNA into E. coli and Analysis with Biotechnology and Bioinformatics Tools

Introduction

The central dogma of molecular biology outlines the flow of genetic information through a biological system. The main aspects include replication of the genetic code (DNA), transcription of DNA into RNA, and translation of RNA into polypeptides which form functional proteins and enzymes. Molecular biologist can manipulate this theory to isolate and multiply a desired trait. This is the basis behind recombinant DNA technology and many pharmaceuticals are produced using these techniques. Following the central dogma, the bacteria will transcribe the new gene into RNA and translate it into a functioning polypeptide that fulfills the scientists needs. Escherichia coli, is a common bacterium found in the human small intestine that aids in digestion and the absorption of vitamins. (Reference about E. coli) E. coli is a model organism for molecular biology research and has played a crucial role in the development of recombinant DNA technology. The basic concepts behind this biotechnology is the insertion of a desired gene into a circular bacterial plasmid DNA using restriction enzymes, transforming the plasmid DNA into living cells, and growing the cells on a culture plate with the desired gene isolated. The goal of these experiments was to produce a EGFP/pET-41a(+) recombinant plasmid that could be transformed into living

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