1 2 3 4 5 6 7 8 9 10 11 Apply genomic fragments to the flow cell Isolate many copies of your candidate mutant's entire genome. Assemble the genomic reads into full length chromosomes based on overlapping sequence Compare the assembled sequence to the reference Saccharomyces cerevisiae genome to identify mismatches Fragment the genome into <500 bp segments Attach DNA "adapters" to each genomic fragment that are complementary to primers on the flow cell Add complementary PCR primer, DNA polymerase, and fluorescently labelled blocked nucleotides Capture the fluorescence emitted by each cluster - the wavelength indicates the identity of the first nucleotide in the fragment Create millions of immobilized "clusters" of unique genomic fragments through bridge amplification Repeat the previous steps between 150-300 additional times to determine the identity of each consecutive base in the genomic fragment Quench the fluorescent signal of the first added dNTP and remove its reversible terminator.
1 2 3 4 5 6 7 8 9 10 11 Apply genomic fragments to the flow cell Isolate many copies of your candidate mutant's entire genome. Assemble the genomic reads into full length chromosomes based on overlapping sequence Compare the assembled sequence to the reference Saccharomyces cerevisiae genome to identify mismatches Fragment the genome into <500 bp segments Attach DNA "adapters" to each genomic fragment that are complementary to primers on the flow cell Add complementary PCR primer, DNA polymerase, and fluorescently labelled blocked nucleotides Capture the fluorescence emitted by each cluster - the wavelength indicates the identity of the first nucleotide in the fragment Create millions of immobilized "clusters" of unique genomic fragments through bridge amplification Repeat the previous steps between 150-300 additional times to determine the identity of each consecutive base in the genomic fragment Quench the fluorescent signal of the first added dNTP and remove its reversible terminator.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Place the steps of whole genome sequencing in order.
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Apply genomic fragments to the flow cell
Isolate many copies of your candidate mutant's entire genome.
Assemble the genomic reads into full length chromosomes based on overlapping sequence
Compare the assembled sequence to the reference Saccharomyces cerevisiae genome to identify mismatches
⠀ Fragment the genome into <500 bp segments
8
⠀ Capture the fluorescence emitted by each cluster - the wavelength indicates the identity of the first nucleotide in the fragment
Attach DNA "adapters" to each genomic fragment that are complementary to primers on the flow cell
Add complementary PCR primer, DNA polymerase, and fluorescently labelled blocked nucleotides
Create millions of immobilized "clusters" of unique genomic fragments through bridge amplification
Repeat the previous steps between 150-300 additional times to determine the identity of each consecutive base in the genomic fragment
Quench the fluorescent signal of the first added dNTP and remove its reversible terminator.
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