1 ml from main culture 1ml 99999 5 ml LB 1 ml 5 ml LB 5ml LB 1ml 5 ml LB 1 ml 5 ml LB You took 250 µl of the last dilution and planted it on solid medium. 50 colonies were counted A total of 10 ml of microbial culture (liquid LB+ e.coli) was brought to your laboratory in a container. This sample contains the bacteria Escherichia coli. The person who brought in wants to know how many colonies (cfu) there are in the culture. You have started the experiment by taking 1 ml from the culture and then added into the first tube which contain 5 ml of LB. After that, you diluted the sample with a dilution rate of 1/6 with 4 times (take 1 ml in 5 ml of LB medium (so total 6 ml)). You took 250 μl of the last dilution and planted it on solid medium and when counted after incubation, 50 colonies were counted. How many cfu/ ml bacteria are there in the sample (main stock) in total? (please show all your calculations and steps). Attach File

1 ml from main culture 1ml 99999 5 ml LB 1 ml 5 ml LB 5ml LB 1ml 5 ml LB 1 ml 5 ml LB You took 250 µl of the last dilution and planted it on solid medium. 50 colonies were counted A total of 10 ml of microbial culture (liquid LB+ e.coli) was brought to your laboratory in a container. This sample contains the bacteria Escherichia coli. The person who brought in wants to know how many colonies (cfu) there are in the culture. You have started the experiment by taking 1 ml from the culture and then added into the first tube which contain 5 ml of LB. After that, you diluted the sample with a dilution rate of 1/6 with 4 times (take 1 ml in 5 ml of LB medium (so total 6 ml)). You took 250 μl of the last dilution and planted it on solid medium and when counted after incubation, 50 colonies were counted. How many cfu/ ml bacteria are there in the sample (main stock) in total? (please show all your calculations and steps). Attach File

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter8: Forms Of Drugs And How They Act

Section: Chapter Questions

Problem 30RQ

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