1. Give the principle involved in the operation of the following instruments and their specific purposes: a. Autoclave b. Hot air Sterilizer c. Inspissator
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- 1. Describe and give the principle of each equipment used for sterilization in microbiology. (refer to equipment drawn only)Describe 3 examples of heavy metal control agents and describe their most common applications.2. Gamma-hemolysis on a blood agar plate would likely appear: Select one: a. As no change in the medium b. Blue c. Yellow d. Green e. As a clearing.
- What is the best method to filter the precipitate from it's reaction mixture? a Gravity filtering is accomplished by folding the filter paper into a cone and placing it in a long stem funnel. b Vacuum filtering using a Buchner funnel with filter paper. c Using a filtering crucible that can be dried and ignited at red heat. d All of the above, just depends on the precipitate.Which of the following would be best for sterilizing a pre-sealed case of plastic medical supplies? A. Autoclave B. Ionization Radiation C. Sonication D. Nonionizing Radiation1. Give the method of sterilization of these laboratory apparatuses how they are sterilized; inoculating loop, glasswares. culture media, skin, and milk. Please cite where you got the information. Thank you
- 1. What are the two ways that nitrate can be reduced by the bacteria. Explain briefly. 2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.1. The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization? 3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilled1.) During a laboratory activity, Chemical reactions from mixture of chemicals should be observed under which laboratory equipment? a.) autoclave b.) fume hood c.) Labaratory Safety cabinet d.) Centrifuge 2.) Which type of equipment contains a HEPA filter that only removes suspended materials from exhausted air? a.) Fume hood b.) Biosafety Cabinet I c.) Biosafety Cabinet II d.) Biosafety Cabinet III
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperCan you give me an example of a specific medium in culture media and fill the information below. General Information Specific a. Name of medium: b. Physical form: c. Category based on formulation: d. Purpose/uses/function: e. List of Ingredients and concentration (per 1000 ml) f. How to prepare?which of the following is a limitation of the autoclave? a. long length of time required for steriliztion b. inability to use with heat sensitive materials c. inability to use with common lab equipment like glassware d. does not sterilize, only sanitized e. all of the above are limitation of the autoclave