What are the two ways that nitrate can be reduced by the bacteria. Explain briefly. 2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.
Q: The number of bacteria in the samples need to be quantified. The laboratory uses a specific protocol…
A: Serial dilution is a process in which we dilute the sample and do passage, transfer, which is…
Q: For each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E…
A: EnteroPluri-Test is a 12-sector system that consists of special culture media. which allows…
Q: What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is…
A: Methyl red test This test usually aims to differentiate the facultative anaerobes enteric bacteria.…
Q: You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria…
A: The bacteria can grow only in the favourable conditions that is when there is optimum amount of pH,…
Q: Please answer it correctly for an Upvote. Thank you. •Explain why cfu/unit analyzed is being used to…
A: CFU stands for colony forming unit and is a measurement unit for estimating the concentration of…
Q: What would be the expected out come of a Nitrate reduction test If the bacteria is a pathogenic…
A: Nitrate Reduction Test is used to determine the ability of an organism to reduce nitrate (NO3) to…
Q: What is the chemical basis of the Xanthoproteic Test? Give the chemical reaction involved.
A: Protein is a nitrogenous organic macromolecule that is essential to human health. It is responsible…
Q: Kligler’s iron agar and SIM are multiple test media.. What component of both media allows you to…
A: Kligler’s Iron Agar (KIA) test is used in differentiating certain members of the Enterobacteriaceae…
Q: Samples subjected to a test were given. Determine the reagent/material in the test. Methyl…
A: Different chemical tests use different reagents and apparatus depending on their working principle.
Q: organisms are being differentiated in the butyrate disc test.
A: The Butyrate test is a rapid detection test for the butyrate esterase enzyme.
Q: Give the uses/functions and images of each apparatuses Basic Laboratory Equipment Uses/Functions…
A: Antibiotic discs are used in microbiological studies to observe the effects of various antibiotics…
Q: Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay…
A: Hematocrit test is used to check the proportion of red blood corposcles (RBC) present. If the RBCs…
Q: Which of the following is TRUE about the Nitrate Reduction test? Question 1 options: A Nitrate…
A: According to the question, we have to mention Which of the following is TRUE about the Nitrate…
Q: Koch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The…
A: Koch's postulates are a series of findings and experimental criteria suggested in the late 1800s by…
Q: Practical techniques used to identify unknown microorganisms’ You should include the types of tests…
A: Microbiology is the study of microorganisms, identification, and classification of a particular…
Q: Make illustrated diagrams of stepwise procedures for the performance of the following tasks. 2.…
A: Microorganisms can be cultured in liquid medium or on solid medium. Culturing on solid medium…
Q: yield
A: The Dische's test is a chemical test that is utilized to detect the presence of DNA in a solution.It…
Q: In the Kirby-Bauer test, which of the following must be consistent? a. concentration of bacteria…
A: Kirby-Bauer test: It is an antibiotic test also known as KB testing. It is used to test which…
Q: Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions…
A: Note- we are supposed to answer three subpart of a question according to our guidelines. Please…
Q: Name one other medical diagnostic are being used in reagent test strips
A: Reagent test strip are diagnostic strips that contain specific reagents for various organ diagnosis…
Q: You saw no color change in both the first step and second step of the Nitrate Reduction test. What…
A: A nitrate reduction test is a test done to test whether the Enterobacteriaceae member produces the…
Q: Provide a brief description and state the function of the following tools and equipment used in the…
A: A laboratory is a facility that provides controlled conditions in which scientific or technological…
Q: 9. Complete the table by transcribing the following culture media, classify as to…
A: Introduction : A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: Which of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation?…
A: Among the many biochemical tests conducted on unknown bacterial isolates to identify them, nitrate…
Q: discuss how the black precipitate is formed in the butt of the test tube during a tripple sugar iron…
A: Triple sugar iron fermentation test is a microbiological test, that test a microorganism’s ability…
Q: What are the examples of organisms that belong to the positive test in in-dose test?
A: Tryptophan is an amino acid that can undergo deamination. Indole is generated by reductive…
Q: Give the importance and purpose of the Cetrimide Test. 2. being performed - Dry Filter Paper…
A: Disclaimer: Since you have asked multipart question I will answer 3 question. Introduction The…
Q: Citrate Test |Cetrimide Agar
A: Biochemical measures are used to differentiate bacterial organisms based on their biochemical…
Q: Citrate test Explain what is the color of the medium after incubation? Green or blue
A: To correctly identify a microbial species, we make use of various biochemical assays that have…
Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…
A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…
Q: explain indole test, startch test and citrate test.
A: Indole test This is a biochemical test that is used to identify the capability of some bacteria to…
Q: Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.
A:
Q: In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For…
A:
Q: 1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C.…
A: The removal of any microorganism or viable spores or bacteria or viruses from the surface. The…
Q: 1. Illustrate in a diagram the identification scheme from gram-positive cocci.
A: Note: We will answer the first question since the exact one was not specified. Please submit a new…
Q: From figure shows triplesugar iron agar. What biochemical characteristics does this…
A: “Triple sugar iron test” is one of the “biochemical tests” used to test the ability of microbe in…
Q: Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big…
A: Petri plates, slants and stabs of Culture media such as Luria Bertani agar is used in microbiology…
Q: Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.
A: Methyl Red (MR) and Voges-Proskauer (VP) broth is used as the medium in which both the Methyl Red…
Q: The colonies on a positive MSA plate would appear ________________. 2. Which of the following tests…
A: Introduction Coliform is the gram-negative bacterial specie that can be motile or non-motile and are…
Q: How can I explain the results of my findings in lamen terms?
A: These test are the perfomed to identify positive and negative result for glucose fermentation.
Q: Other than the PYR test, what is another use of the PEP reagent?
A: PYR is a colorimetric method used to detect the presence of pyrolidonyl arylamidase activity in a…
Q: Which one of the following methods of antimicrobial susceptibility testing combines some of the…
A: Antimicrobial susceptibility tests are performed to identify which drugs are effective against a…
Q: ther than the application of heat, name other methods of sterilization. Define the methods and give…
A: Sterilization is the process of completely destroying all microorganisms on the surface of an…
Q: Describe the process of qualitative water test for coliform contamination. If shown EMB plates, be…
A: * Coliform bacteria are Bacteria and stains Gram negative and they can be motile or non motile…
Q: Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions…
A: There are many Laboratory Equipment which are used in school , collages and for various scientific…
Q: Clear tubes in the MIC test were inoculated in Mueller-Hinton Agar to determine the MBC of the…
A: MBC - stands for minimum bactericidal concentration It is the lowest concentration of antibiotics…
Q: You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture.…
A: Answer : First step of the kirby-Bauer test :-
Q: a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria
A: Biochemical test such as nitrate reduction test and phenol red test are used in the identification…
1. What are the two ways that nitrate can be reduced by the bacteria. Explain briefly.
2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- 2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.write a detailed description of each of the following tests/media: a. TSI Agar Slant b. SIM Medium c. Urease Test d. The IMViC Tests e. The Catalase Test f. The Oxidase TestNitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.
- 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.From figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?Explain the mechanism leading to the given reactions in the inoculated biochemical media. 1. Blackening of the medium in Triple Sugar Iron Test slant2. Development of pink to red color in nitrate broth in Nitrate Reduction Test
- What would be the expected out come of a Nitrate reduction test If the bacteria is a pathogenic intestinal bacteria?1. One gram of sludge was added to a 99 mL dilution blank. Three 1/10 dilutions were then made. From the last dilution made, 200 µL was plated onto an all-purpose medium. After incubation, 123 colonies were counted. What is the CFU/mL in the sludge? Note: 1 gram sludge is equal to 1 mL sludge.1. Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.
- how do microorganisms such as bacteria eliminate harmful reactive oxygen species? Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.Which of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation? Question 2 options: A Nitrate medium contains 0.1% potassium nitrate as a substrate B Reagent A and B are used to determine the presence of nitrite C When zinc is added, it will react with any nitrate and turn red D A tube with red after zinc is a positive test for nitrate reduction E A tube that turns red after reagent A and B is a positive test3.1 You can perform a specific staining method to confirm the purity on the culture. Describe this staining method. 3.2 Describe the results obtained from 3.1 and comment about the purity of the culture. 3.3 What staining method would you use to verify that the isolate is not producing survivalstructures against unfavourable conditions? Describe the procedure as well.
