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1. List four general methods for experimentally inhibiting the activity of a specific gene with citations.
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- 1. a)how is it possible for such drugs to selectively kill bacterial cells and not our own cells? b)Provide an example of post-translational regulation of protein activity and explain the advantage of regulating each protein/process at the post-translational level instead of the transcriptional level.9) Describe the steps required to use sequencing DNA to identify your gene of interest after performing a forward genetic screen.1. What is the function of the CRISPR/ Cas system 2. Briefly outline the components of the CRISPR/Cas system
- 1. Propose a way that a chi square analysis could be used in other experiments, such as genetics ordrug trials.1. How can site-specific recombination be used in recombinant DNA technology? Answer and explain comprehensively.Besides the great potential of gene editing, what are the biggest risks?  How do scientist test to ensure their gene editing experiments target only the desired specific genes?
- 1. What is Transcriptome? What is Transcriptome analysis? How does the method yield information able to elucidate the structural apexes of the molecules under examination? What are the steps and primary methods used in Transcriptome analysis? What are some important applications of Transcriptome analysis?Please be as detailed as you could be in your explanation.1. What are the different elements you would need if you wanted to perform CRISPR to edit a gene? 2. What are some of the ways CRISPR technology can benefit humans?1. What is the purpose of adding of (a) warm salt water, (b) detergent, and (c) alcohol on the experiment of the extraction of DNA from a banana?
- The ab initio approach finds genes by looking for a. common sequences found in most genes. b. similarity in sequence with known genes. c. mRNA with the use of in situ hybridization. d. mutant phenotypes1. Suppose Biuret test is conducted to a solution of RNA. Will it give a positive result or not? Explain your answer.1. what are the possible probes of a 15- nucleotide length that would be used in a PCR reaction that could introduce a codon change to the amino acid substitution. Which one should be used and why?